The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. / Sparmann, Anke; Xie, Yunli; Verhoeven, Els; Vermeulen, Michiel; Lancini, Cesare; Gargiulo, Gaetano; Hulsman, Danielle; Mann, Matthias; Knoblich, Juergen A; van Lohuizen, Maarten.

In: E M B O Journal, Vol. 32, No. 11, 29.05.2013, p. 1598-612.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sparmann, A, Xie, Y, Verhoeven, E, Vermeulen, M, Lancini, C, Gargiulo, G, Hulsman, D, Mann, M, Knoblich, JA & van Lohuizen, M 2013, 'The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation', E M B O Journal, vol. 32, no. 11, pp. 1598-612. https://doi.org/10.1038/emboj.2013.93

APA

Sparmann, A., Xie, Y., Verhoeven, E., Vermeulen, M., Lancini, C., Gargiulo, G., Hulsman, D., Mann, M., Knoblich, J. A., & van Lohuizen, M. (2013). The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. E M B O Journal, 32(11), 1598-612. https://doi.org/10.1038/emboj.2013.93

Vancouver

Sparmann A, Xie Y, Verhoeven E, Vermeulen M, Lancini C, Gargiulo G et al. The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. E M B O Journal. 2013 May 29;32(11):1598-612. https://doi.org/10.1038/emboj.2013.93

Author

Sparmann, Anke ; Xie, Yunli ; Verhoeven, Els ; Vermeulen, Michiel ; Lancini, Cesare ; Gargiulo, Gaetano ; Hulsman, Danielle ; Mann, Matthias ; Knoblich, Juergen A ; van Lohuizen, Maarten. / The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. In: E M B O Journal. 2013 ; Vol. 32, No. 11. pp. 1598-612.

Bibtex

@article{9bca7b36615d4d57ab4cfaf7e1a37a2e,
title = "The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation",
abstract = "Polycomb group (PcG) proteins form transcriptional repressor complexes with well-established functions during cell-fate determination. Yet, the mechanisms underlying their regulation remain poorly understood. Here, we extend the role of Polycomb complexes in the temporal control of neural progenitor cell (NPC) commitment by demonstrating that the PcG protein Ezh2 is necessary to prevent the premature onset of gliogenesis. In addition, we identify the chromodomain helicase DNA-binding protein 4 (Chd4) as a critical interaction partner of Ezh2 required specifically for PcG-mediated suppression of the key astrogenic marker gene GFAP. Accordingly, in vivo depletion of Chd4 in the developing neocortex promotes astrogenesis. Collectively, these results demonstrate that PcG proteins operate in a highly dynamic, developmental stage-dependent fashion during neural differentiation and suggest that target gene-specific mechanisms regulate Polycomb function during sequential cell-fate decisions.",
keywords = "Animals, Astrocytes, Cell Differentiation, Cell Line, Chromatin Immunoprecipitation, DNA Helicases, Embryo, Mammalian, Female, Gene Expression Regulation, Developmental, Histones, Mice, Mice, Inbred C57BL, Nerve Tissue Proteins, Neural Stem Cells, Nuclear Proteins, Polycomb Repressive Complex 2, Polycomb-Group Proteins, Pregnancy, Promoter Regions, Genetic",
author = "Anke Sparmann and Yunli Xie and Els Verhoeven and Michiel Vermeulen and Cesare Lancini and Gaetano Gargiulo and Danielle Hulsman and Matthias Mann and Knoblich, {Juergen A} and {van Lohuizen}, Maarten",
year = "2013",
month = may,
day = "29",
doi = "10.1038/emboj.2013.93",
language = "English",
volume = "32",
pages = "1598--612",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "11",

}

RIS

TY - JOUR

T1 - The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation

AU - Sparmann, Anke

AU - Xie, Yunli

AU - Verhoeven, Els

AU - Vermeulen, Michiel

AU - Lancini, Cesare

AU - Gargiulo, Gaetano

AU - Hulsman, Danielle

AU - Mann, Matthias

AU - Knoblich, Juergen A

AU - van Lohuizen, Maarten

PY - 2013/5/29

Y1 - 2013/5/29

N2 - Polycomb group (PcG) proteins form transcriptional repressor complexes with well-established functions during cell-fate determination. Yet, the mechanisms underlying their regulation remain poorly understood. Here, we extend the role of Polycomb complexes in the temporal control of neural progenitor cell (NPC) commitment by demonstrating that the PcG protein Ezh2 is necessary to prevent the premature onset of gliogenesis. In addition, we identify the chromodomain helicase DNA-binding protein 4 (Chd4) as a critical interaction partner of Ezh2 required specifically for PcG-mediated suppression of the key astrogenic marker gene GFAP. Accordingly, in vivo depletion of Chd4 in the developing neocortex promotes astrogenesis. Collectively, these results demonstrate that PcG proteins operate in a highly dynamic, developmental stage-dependent fashion during neural differentiation and suggest that target gene-specific mechanisms regulate Polycomb function during sequential cell-fate decisions.

AB - Polycomb group (PcG) proteins form transcriptional repressor complexes with well-established functions during cell-fate determination. Yet, the mechanisms underlying their regulation remain poorly understood. Here, we extend the role of Polycomb complexes in the temporal control of neural progenitor cell (NPC) commitment by demonstrating that the PcG protein Ezh2 is necessary to prevent the premature onset of gliogenesis. In addition, we identify the chromodomain helicase DNA-binding protein 4 (Chd4) as a critical interaction partner of Ezh2 required specifically for PcG-mediated suppression of the key astrogenic marker gene GFAP. Accordingly, in vivo depletion of Chd4 in the developing neocortex promotes astrogenesis. Collectively, these results demonstrate that PcG proteins operate in a highly dynamic, developmental stage-dependent fashion during neural differentiation and suggest that target gene-specific mechanisms regulate Polycomb function during sequential cell-fate decisions.

KW - Animals

KW - Astrocytes

KW - Cell Differentiation

KW - Cell Line

KW - Chromatin Immunoprecipitation

KW - DNA Helicases

KW - Embryo, Mammalian

KW - Female

KW - Gene Expression Regulation, Developmental

KW - Histones

KW - Mice

KW - Mice, Inbred C57BL

KW - Nerve Tissue Proteins

KW - Neural Stem Cells

KW - Nuclear Proteins

KW - Polycomb Repressive Complex 2

KW - Polycomb-Group Proteins

KW - Pregnancy

KW - Promoter Regions, Genetic

U2 - 10.1038/emboj.2013.93

DO - 10.1038/emboj.2013.93

M3 - Journal article

C2 - 23624931

VL - 32

SP - 1598

EP - 1612

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 11

ER -

ID: 88584618