The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling

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Standard

The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling. / Altmeyer, Matthias; Toledo Lazaro, Luis Ignacio; Gudjonsson, Thorkell; Grøfte, Merete; Rask, Maj-Britt; Lukas, Claudia; Akimov, Vyacheslav; Blagoev, Blagoy; Bartek, Jiri; Lukas, Jiri.

In: Molecular Cell, Vol. 52, No. 2, 24.10.2013, p. 206-220.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Altmeyer, M, Toledo Lazaro, LI, Gudjonsson, T, Grøfte, M, Rask, M-B, Lukas, C, Akimov, V, Blagoev, B, Bartek, J & Lukas, J 2013, 'The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling', Molecular Cell, vol. 52, no. 2, pp. 206-220. https://doi.org/10.1016/j.molcel.2013.08.025

APA

Altmeyer, M., Toledo Lazaro, L. I., Gudjonsson, T., Grøfte, M., Rask, M-B., Lukas, C., Akimov, V., Blagoev, B., Bartek, J., & Lukas, J. (2013). The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling. Molecular Cell, 52(2), 206-220. https://doi.org/10.1016/j.molcel.2013.08.025

Vancouver

Altmeyer M, Toledo Lazaro LI, Gudjonsson T, Grøfte M, Rask M-B, Lukas C et al. The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling. Molecular Cell. 2013 Oct 24;52(2):206-220. https://doi.org/10.1016/j.molcel.2013.08.025

Author

Altmeyer, Matthias ; Toledo Lazaro, Luis Ignacio ; Gudjonsson, Thorkell ; Grøfte, Merete ; Rask, Maj-Britt ; Lukas, Claudia ; Akimov, Vyacheslav ; Blagoev, Blagoy ; Bartek, Jiri ; Lukas, Jiri. / The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling. In: Molecular Cell. 2013 ; Vol. 52, No. 2. pp. 206-220.

Bibtex

@article{efa9f3c2668748418455ebbd671535e5,
title = "The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling",
abstract = "Although the general relevance of chromatin modifications for genotoxic stress signaling, cell-cycle checkpoint activation, and DNA repair is well established, how these modifications reach initial thresholds in order to trigger robust responses remains largely unexplored. Here, we identify the chromatin-associated scaffold attachment factor SAFB1 as a component of the DNA damage response and show that SAFB1 cooperates with histone acetylation to allow for efficient γH2AX spreading and genotoxic stress signaling. SAFB1 undergoes a highly dynamic exchange at damaged chromatin in a poly(ADP-ribose)-polymerase 1- and poly(ADP-ribose)-dependent manner and is required for unperturbed cell-cycle checkpoint activation and guarding cells against replicative stress. Altogether, our data reveal that transient recruitment of an architectural chromatin component is required in order to overcome physiological barriers by making chromatin permissive for DNA damage signaling, whereas the ensuing exclusion of SAFB1 may help prevent excessive signaling.",
author = "Matthias Altmeyer and {Toledo Lazaro}, {Luis Ignacio} and Thorkell Gudjonsson and Merete Gr{\o}fte and Maj-Britt Rask and Claudia Lukas and Vyacheslav Akimov and Blagoy Blagoev and Jiri Bartek and Jiri Lukas",
note = "Copyright {\textcopyright} 2013 Elsevier Inc. All rights reserved.",
year = "2013",
month = oct,
day = "24",
doi = "10.1016/j.molcel.2013.08.025",
language = "English",
volume = "52",
pages = "206--220",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - The Chromatin Scaffold Protein SAFB1 Renders Chromatin Permissive for DNA Damage Signaling

AU - Altmeyer, Matthias

AU - Toledo Lazaro, Luis Ignacio

AU - Gudjonsson, Thorkell

AU - Grøfte, Merete

AU - Rask, Maj-Britt

AU - Lukas, Claudia

AU - Akimov, Vyacheslav

AU - Blagoev, Blagoy

AU - Bartek, Jiri

AU - Lukas, Jiri

N1 - Copyright © 2013 Elsevier Inc. All rights reserved.

PY - 2013/10/24

Y1 - 2013/10/24

N2 - Although the general relevance of chromatin modifications for genotoxic stress signaling, cell-cycle checkpoint activation, and DNA repair is well established, how these modifications reach initial thresholds in order to trigger robust responses remains largely unexplored. Here, we identify the chromatin-associated scaffold attachment factor SAFB1 as a component of the DNA damage response and show that SAFB1 cooperates with histone acetylation to allow for efficient γH2AX spreading and genotoxic stress signaling. SAFB1 undergoes a highly dynamic exchange at damaged chromatin in a poly(ADP-ribose)-polymerase 1- and poly(ADP-ribose)-dependent manner and is required for unperturbed cell-cycle checkpoint activation and guarding cells against replicative stress. Altogether, our data reveal that transient recruitment of an architectural chromatin component is required in order to overcome physiological barriers by making chromatin permissive for DNA damage signaling, whereas the ensuing exclusion of SAFB1 may help prevent excessive signaling.

AB - Although the general relevance of chromatin modifications for genotoxic stress signaling, cell-cycle checkpoint activation, and DNA repair is well established, how these modifications reach initial thresholds in order to trigger robust responses remains largely unexplored. Here, we identify the chromatin-associated scaffold attachment factor SAFB1 as a component of the DNA damage response and show that SAFB1 cooperates with histone acetylation to allow for efficient γH2AX spreading and genotoxic stress signaling. SAFB1 undergoes a highly dynamic exchange at damaged chromatin in a poly(ADP-ribose)-polymerase 1- and poly(ADP-ribose)-dependent manner and is required for unperturbed cell-cycle checkpoint activation and guarding cells against replicative stress. Altogether, our data reveal that transient recruitment of an architectural chromatin component is required in order to overcome physiological barriers by making chromatin permissive for DNA damage signaling, whereas the ensuing exclusion of SAFB1 may help prevent excessive signaling.

U2 - 10.1016/j.molcel.2013.08.025

DO - 10.1016/j.molcel.2013.08.025

M3 - Journal article

C2 - 24055346

VL - 52

SP - 206

EP - 220

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 2

ER -

ID: 57420635