SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage

Research output: Contribution to journal › Journal article › Research › peer-review

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SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. / Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V; Vertegaal, Alfred C O.

In: Cell Reports, Vol. 10, No. 10, 17.03.2015, p. 1778–1791.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hendriks, IA, Treffers, LW, Verlaan-de Vries, M, Olsen, JV & Vertegaal, ACO 2015, 'SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage', Cell Reports, vol. 10, no. 10, pp. 1778–1791. https://doi.org/10.1016/j.celrep.2015.02.033

APA

Hendriks, I. A., Treffers, L. W., Verlaan-de Vries, M., Olsen, J. V., & Vertegaal, A. C. O. (2015). SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Reports, 10(10), 1778–1791. https://doi.org/10.1016/j.celrep.2015.02.033

Vancouver

Hendriks IA, Treffers LW, Verlaan-de Vries M, Olsen JV, Vertegaal ACO. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Reports. 2015 Mar 17;10(10): 1778–1791. https://doi.org/10.1016/j.celrep.2015.02.033

Author

Hendriks, Ivo A ; Treffers, Louise W ; Verlaan-de Vries, Matty ; Olsen, Jesper V ; Vertegaal, Alfred C O. / SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. In: Cell Reports. 2015 ; Vol. 10, No. 10. pp. 1778–1791.

Bibtex

@article{463113b948a748ab9f2209d0a9cc76cb,
title = "SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage",
abstract = "Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.",
author = "Hendriks, {Ivo A} and Treffers, {Louise W} and {Verlaan-de Vries}, Matty and Olsen, {Jesper V} and Vertegaal, {Alfred C O}",
note = "Copyright {\textcopyright} 2015 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2015",
month = mar,
day = "17",
doi = "10.1016/j.celrep.2015.02.033",
language = "English",
volume = "10",
pages = " 1778–1791",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage

AU - Hendriks, Ivo A

AU - Treffers, Louise W

AU - Verlaan-de Vries, Matty

AU - Olsen, Jesper V

AU - Vertegaal, Alfred C O

N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2015/3/17

Y1 - 2015/3/17

N2 - Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.

AB - Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.

U2 - 10.1016/j.celrep.2015.02.033

DO - 10.1016/j.celrep.2015.02.033

M3 - Journal article

C2 - 25772364

VL - 10

SP - 1778

EP - 1791

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 10

ER -

ID: 139976382