Structural mechanism of extranucleosomal DNA readout by the INO80 complex

Research output: Contribution to journal › Journal article › Research › peer-review

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Structural mechanism of extranucleosomal DNA readout by the INO80 complex. / Kunert, Franziska; Metzner, Felix J.; Jung, James; Hopfler, Markus; Woike, Stephan; Schall, Kevin; Kostrewa, Dirk; Moldt, Manuela; Chen, Jia Xuan; Bantele, Susanne; Pfander, Boris; Eustermann, Sebastian; Hopfner, Karl Peter.

In: Science Advances, Vol. 8, No. 49, add3189, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kunert, F, Metzner, FJ, Jung, J, Hopfler, M, Woike, S, Schall, K, Kostrewa, D, Moldt, M, Chen, JX, Bantele, S, Pfander, B, Eustermann, S & Hopfner, KP 2022, 'Structural mechanism of extranucleosomal DNA readout by the INO80 complex', Science Advances, vol. 8, no. 49, add3189. https://doi.org/10.1126/sciadv.add3189

APA

Kunert, F., Metzner, F. J., Jung, J., Hopfler, M., Woike, S., Schall, K., Kostrewa, D., Moldt, M., Chen, J. X., Bantele, S., Pfander, B., Eustermann, S., & Hopfner, K. P. (2022). Structural mechanism of extranucleosomal DNA readout by the INO80 complex. Science Advances, 8(49), [add3189]. https://doi.org/10.1126/sciadv.add3189

Vancouver

Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K et al. Structural mechanism of extranucleosomal DNA readout by the INO80 complex. Science Advances. 2022;8(49). add3189. https://doi.org/10.1126/sciadv.add3189

Author

Kunert, Franziska ; Metzner, Felix J. ; Jung, James ; Hopfler, Markus ; Woike, Stephan ; Schall, Kevin ; Kostrewa, Dirk ; Moldt, Manuela ; Chen, Jia Xuan ; Bantele, Susanne ; Pfander, Boris ; Eustermann, Sebastian ; Hopfner, Karl Peter. / Structural mechanism of extranucleosomal DNA readout by the INO80 complex. In: Science Advances. 2022 ; Vol. 8, No. 49.

Bibtex

@article{3d626f34e96d4a9bb2d95b5d5b95859c,
title = "Structural mechanism of extranucleosomal DNA readout by the INO80 complex",
abstract = "The nucleosomal landscape of chromatin depends on the concerted action of chromatin remodelers. The INO80 remodeler specifically places nucleosomes at the boundary of gene regulatory elements, which is proposed to be the result of an ATP-dependent nucleosome sliding activity that is regulated by extranucleosomal DNA features. Here, we use cryo-electron microscopy and functional assays to reveal how INO80 binds and is regulated by extranucleosomal DNA. Structures of the regulatory A-module bound to DNA clarify the mechanism of linker DNA binding. The A-module is connected to the motor unit via an HSA/post-HSA lever element to chemomechanically couple the motor and linker DNA sensing. Two notable sites of curved DNA recognition by coordinated action of the four actin/actin-related proteins and the motor suggest how sliding by INO80 can be regulated by extranucleosomal DNA features. Last, the structures clarify the recruitment of YY1/Ies4 subunits and reveal deep architectural similarities between the regulatory modules of INO80 and SWI/SNF complexes.",
author = "Franziska Kunert and Metzner, {Felix J.} and James Jung and Markus Hopfler and Stephan Woike and Kevin Schall and Dirk Kostrewa and Manuela Moldt and Chen, {Jia Xuan} and Susanne Bantele and Boris Pfander and Sebastian Eustermann and Hopfner, {Karl Peter}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors.",
year = "2022",
doi = "10.1126/sciadv.add3189",
language = "English",
volume = "8",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "49",

}

RIS

TY - JOUR

T1 - Structural mechanism of extranucleosomal DNA readout by the INO80 complex

AU - Kunert, Franziska

AU - Metzner, Felix J.

AU - Jung, James

AU - Hopfler, Markus

AU - Woike, Stephan

AU - Schall, Kevin

AU - Kostrewa, Dirk

AU - Moldt, Manuela

AU - Chen, Jia Xuan

AU - Bantele, Susanne

AU - Pfander, Boris

AU - Eustermann, Sebastian

AU - Hopfner, Karl Peter

N1 - Publisher Copyright: © 2022 The Authors.

PY - 2022

Y1 - 2022

N2 - The nucleosomal landscape of chromatin depends on the concerted action of chromatin remodelers. The INO80 remodeler specifically places nucleosomes at the boundary of gene regulatory elements, which is proposed to be the result of an ATP-dependent nucleosome sliding activity that is regulated by extranucleosomal DNA features. Here, we use cryo-electron microscopy and functional assays to reveal how INO80 binds and is regulated by extranucleosomal DNA. Structures of the regulatory A-module bound to DNA clarify the mechanism of linker DNA binding. The A-module is connected to the motor unit via an HSA/post-HSA lever element to chemomechanically couple the motor and linker DNA sensing. Two notable sites of curved DNA recognition by coordinated action of the four actin/actin-related proteins and the motor suggest how sliding by INO80 can be regulated by extranucleosomal DNA features. Last, the structures clarify the recruitment of YY1/Ies4 subunits and reveal deep architectural similarities between the regulatory modules of INO80 and SWI/SNF complexes.

AB - The nucleosomal landscape of chromatin depends on the concerted action of chromatin remodelers. The INO80 remodeler specifically places nucleosomes at the boundary of gene regulatory elements, which is proposed to be the result of an ATP-dependent nucleosome sliding activity that is regulated by extranucleosomal DNA features. Here, we use cryo-electron microscopy and functional assays to reveal how INO80 binds and is regulated by extranucleosomal DNA. Structures of the regulatory A-module bound to DNA clarify the mechanism of linker DNA binding. The A-module is connected to the motor unit via an HSA/post-HSA lever element to chemomechanically couple the motor and linker DNA sensing. Two notable sites of curved DNA recognition by coordinated action of the four actin/actin-related proteins and the motor suggest how sliding by INO80 can be regulated by extranucleosomal DNA features. Last, the structures clarify the recruitment of YY1/Ies4 subunits and reveal deep architectural similarities between the regulatory modules of INO80 and SWI/SNF complexes.

U2 - 10.1126/sciadv.add3189

DO - 10.1126/sciadv.add3189

M3 - Journal article

C2 - 36490333

AN - SCOPUS:85143917546

VL - 8

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 49

M1 - add3189

ER -

ID: 336465026