Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint

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Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. / Hein, Jamin B; Nilsson, Jakob.

In: E M B O Reports, Vol. 15, 25.01.2014, p. 264-272.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hein, JB & Nilsson, J 2014, 'Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint', E M B O Reports, vol. 15, pp. 264-272. https://doi.org/10.1002/embr.201337496

APA

Hein, J. B., & Nilsson, J. (2014). Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. E M B O Reports, 15, 264-272. https://doi.org/10.1002/embr.201337496

Vancouver

Hein JB, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. E M B O Reports. 2014 Jan 25;15:264-272. https://doi.org/10.1002/embr.201337496

Author

Hein, Jamin B ; Nilsson, Jakob. / Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. In: E M B O Reports. 2014 ; Vol. 15. pp. 264-272.

Bibtex

@article{857ceda310b842f5af3c7ec1637e947b,
title = "Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint",
abstract = "The spindle assembly checkpoint (SAC) delays progression into anaphase until all chromosomes have aligned on the metaphase plate by inhibiting Cdc20, the mitotic co-activator of the APC/C. Mad2 and BubR1 bind and inhibit Cdc20, thereby forming the mitotic checkpoint complex (MCC), which can bind stably to the APC/C. Whether MCC formation per se is sufficient for a functional SAC or MCC association with the APC/C is required remains unclear. Here, we analyze the role of two conserved motifs in Cdc20, IR and C-Box, in binding of the MCC to the APC/C. Mutants in both motifs assemble the MCC normally, but IR motif integrity is particularly important for stable binding to the APC/C. Cells expressing Cdc20 with a mutated IR motif have a compromised SAC, as uninhibited Cdc20 can compete with the MCC for APC/C binding and activate it. We thus show that stable MCC association with the APC/C is critical for a functional SAC.",
author = "Hein, {Jamin B} and Jakob Nilsson",
year = "2014",
month = jan,
day = "25",
doi = "10.1002/embr.201337496",
language = "English",
volume = "15",
pages = "264--272",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint

AU - Hein, Jamin B

AU - Nilsson, Jakob

PY - 2014/1/25

Y1 - 2014/1/25

N2 - The spindle assembly checkpoint (SAC) delays progression into anaphase until all chromosomes have aligned on the metaphase plate by inhibiting Cdc20, the mitotic co-activator of the APC/C. Mad2 and BubR1 bind and inhibit Cdc20, thereby forming the mitotic checkpoint complex (MCC), which can bind stably to the APC/C. Whether MCC formation per se is sufficient for a functional SAC or MCC association with the APC/C is required remains unclear. Here, we analyze the role of two conserved motifs in Cdc20, IR and C-Box, in binding of the MCC to the APC/C. Mutants in both motifs assemble the MCC normally, but IR motif integrity is particularly important for stable binding to the APC/C. Cells expressing Cdc20 with a mutated IR motif have a compromised SAC, as uninhibited Cdc20 can compete with the MCC for APC/C binding and activate it. We thus show that stable MCC association with the APC/C is critical for a functional SAC.

AB - The spindle assembly checkpoint (SAC) delays progression into anaphase until all chromosomes have aligned on the metaphase plate by inhibiting Cdc20, the mitotic co-activator of the APC/C. Mad2 and BubR1 bind and inhibit Cdc20, thereby forming the mitotic checkpoint complex (MCC), which can bind stably to the APC/C. Whether MCC formation per se is sufficient for a functional SAC or MCC association with the APC/C is required remains unclear. Here, we analyze the role of two conserved motifs in Cdc20, IR and C-Box, in binding of the MCC to the APC/C. Mutants in both motifs assemble the MCC normally, but IR motif integrity is particularly important for stable binding to the APC/C. Cells expressing Cdc20 with a mutated IR motif have a compromised SAC, as uninhibited Cdc20 can compete with the MCC for APC/C binding and activate it. We thus show that stable MCC association with the APC/C is critical for a functional SAC.

U2 - 10.1002/embr.201337496

DO - 10.1002/embr.201337496

M3 - Journal article

C2 - 24464857

VL - 15

SP - 264

EP - 272

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

ER -

ID: 97277176