Sleep-wake cycles drive daily dynamics of synaptic phosphorylation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. / Brüning, Franziska; Noya, Sara B; Bange, Tanja; Koutsouli, Stella; Rudolph, Jan D; Tyagarajan, Shiva K; Cox, Jürgen; Mann, Matthias; Brown, Steven A; Robles, Maria S.

In: Science (New York, N.Y.), Vol. 366, No. 6462, eaav3617 , 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Brüning, F, Noya, SB, Bange, T, Koutsouli, S, Rudolph, JD, Tyagarajan, SK, Cox, J, Mann, M, Brown, SA & Robles, MS 2019, 'Sleep-wake cycles drive daily dynamics of synaptic phosphorylation', Science (New York, N.Y.), vol. 366, no. 6462, eaav3617 . https://doi.org/10.1126/science.aav3617

APA

Brüning, F., Noya, S. B., Bange, T., Koutsouli, S., Rudolph, J. D., Tyagarajan, S. K., Cox, J., Mann, M., Brown, S. A., & Robles, M. S. (2019). Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. Science (New York, N.Y.), 366(6462), [eaav3617 ]. https://doi.org/10.1126/science.aav3617

Vancouver

Brüning F, Noya SB, Bange T, Koutsouli S, Rudolph JD, Tyagarajan SK et al. Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. Science (New York, N.Y.). 2019;366(6462). eaav3617 . https://doi.org/10.1126/science.aav3617

Author

Brüning, Franziska ; Noya, Sara B ; Bange, Tanja ; Koutsouli, Stella ; Rudolph, Jan D ; Tyagarajan, Shiva K ; Cox, Jürgen ; Mann, Matthias ; Brown, Steven A ; Robles, Maria S. / Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. In: Science (New York, N.Y.). 2019 ; Vol. 366, No. 6462.

Bibtex

@article{6dcf641d048342c1ac67c26268b8488f,
title = "Sleep-wake cycles drive daily dynamics of synaptic phosphorylation",
abstract = "The circadian clock drives daily changes of physiology, including sleep-wake cycles, through regulation of transcription, protein abundance, and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24 hours, accurately quantifying almost 8000 phosphopeptides. Half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function through phosphorylation, including synaptic transmission, cytoskeleton reorganization, and excitatory/inhibitory balance. Sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.",
author = "Franziska Br{\"u}ning and Noya, {Sara B} and Tanja Bange and Stella Koutsouli and Rudolph, {Jan D} and Tyagarajan, {Shiva K} and J{\"u}rgen Cox and Matthias Mann and Brown, {Steven A} and Robles, {Maria S}",
year = "2019",
doi = "10.1126/science.aav3617",
language = "English",
volume = "366",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6462",

}

RIS

TY - JOUR

T1 - Sleep-wake cycles drive daily dynamics of synaptic phosphorylation

AU - Brüning, Franziska

AU - Noya, Sara B

AU - Bange, Tanja

AU - Koutsouli, Stella

AU - Rudolph, Jan D

AU - Tyagarajan, Shiva K

AU - Cox, Jürgen

AU - Mann, Matthias

AU - Brown, Steven A

AU - Robles, Maria S

PY - 2019

Y1 - 2019

N2 - The circadian clock drives daily changes of physiology, including sleep-wake cycles, through regulation of transcription, protein abundance, and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24 hours, accurately quantifying almost 8000 phosphopeptides. Half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function through phosphorylation, including synaptic transmission, cytoskeleton reorganization, and excitatory/inhibitory balance. Sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.

AB - The circadian clock drives daily changes of physiology, including sleep-wake cycles, through regulation of transcription, protein abundance, and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24 hours, accurately quantifying almost 8000 phosphopeptides. Half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function through phosphorylation, including synaptic transmission, cytoskeleton reorganization, and excitatory/inhibitory balance. Sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.

U2 - 10.1126/science.aav3617

DO - 10.1126/science.aav3617

M3 - Journal article

C2 - 31601740

VL - 366

JO - Science

JF - Science

SN - 0036-8075

IS - 6462

M1 - eaav3617

ER -

ID: 229855784