Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana.

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Standard

Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana. / Schubert, Veit; Klatte, Marco; Pecinka, Ales; Meister, Armin; Jasencakova, Zusana; Schubert, Ingo.

In: Genetics, Vol. 172, No. 1, 2005, p. 467-75.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schubert, V, Klatte, M, Pecinka, A, Meister, A, Jasencakova, Z & Schubert, I 2005, 'Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana.', Genetics, vol. 172, no. 1, pp. 467-75. https://doi.org/10.1534/genetics.105.048363

APA

Schubert, V., Klatte, M., Pecinka, A., Meister, A., Jasencakova, Z., & Schubert, I. (2005). Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana. Genetics, 172(1), 467-75. https://doi.org/10.1534/genetics.105.048363

Vancouver

Schubert V, Klatte M, Pecinka A, Meister A, Jasencakova Z, Schubert I. Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana. Genetics. 2005;172(1):467-75. https://doi.org/10.1534/genetics.105.048363

Author

Schubert, Veit ; Klatte, Marco ; Pecinka, Ales ; Meister, Armin ; Jasencakova, Zusana ; Schubert, Ingo. / Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana. In: Genetics. 2005 ; Vol. 172, No. 1. pp. 467-75.

Bibtex

@article{246cb170518b11dd8d9f000ea68e967b,
title = "Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana.",
abstract = "We analyzed whether sister chromatids are continuously aligned in meristematic and endopolyploid Arabidopsis interphase nuclei by studying sister-chromatid alignment at various chromosomal positions. FISH with individual BACs to flow-sorted 4C root and leaf nuclei frequently yielded more than two hybridization signals, indicating incomplete or absent sister-chromatid alignment. Up to 100% of 8C, 16C, and 32C nuclei showed no sister-chromatid alignment at defined positions. Simultaneous FISH with BACs from different chromosomal positions revealed more frequent sister-chromatid alignment in terminal than in midarm positions. Centromeric positions were mainly aligned up to a ploidy level of 16C but became separated or dispersed in 32C nuclei. DNA hypomethylation (of the whole genome) and transcriptional activity (at FWA gene position) did not impair sister-chromatid alignment. Only 6.1% of 4C leaf nuclei showed sister-chromatid separation of the entire chromosome 1 top arm territories. Homozygous transgenic tandem repeat (lac operator) arrays showing somatic homologous pairing more often than average euchromatic loci did not promote an increased frequency of sister-chromatid alignment. The high frequency of separated sister-chromatid arm positions in > or =4C nuclei suggests that sister-chromatid cohesion is variable, dynamic, and not obligatory along the entire chromosome arm in meristematic and differentiated Arabidopsis nuclei.",
author = "Veit Schubert and Marco Klatte and Ales Pecinka and Armin Meister and Zusana Jasencakova and Ingo Schubert",
note = "Keywords: Arabidopsis; Cell Nucleus; Centromere; Chromosomes, Plant; DNA Methylation; DNA, Plant; Genome, Plant; Interphase; Lac Operon; Meristem; Ploidies; Sister Chromatid Exchange; Tandem Repeat Sequences",
year = "2005",
doi = "10.1534/genetics.105.048363",
language = "English",
volume = "172",
pages = "467--75",
journal = "Genetics",
issn = "1943-2631",
publisher = "The Genetics Society of America (GSA)",
number = "1",

}

RIS

TY - JOUR

T1 - Sister chromatids are often incompletely aligned in meristematic and endopolyploid interphase nuclei of Arabidopsis thaliana.

AU - Schubert, Veit

AU - Klatte, Marco

AU - Pecinka, Ales

AU - Meister, Armin

AU - Jasencakova, Zusana

AU - Schubert, Ingo

N1 - Keywords: Arabidopsis; Cell Nucleus; Centromere; Chromosomes, Plant; DNA Methylation; DNA, Plant; Genome, Plant; Interphase; Lac Operon; Meristem; Ploidies; Sister Chromatid Exchange; Tandem Repeat Sequences

PY - 2005

Y1 - 2005

N2 - We analyzed whether sister chromatids are continuously aligned in meristematic and endopolyploid Arabidopsis interphase nuclei by studying sister-chromatid alignment at various chromosomal positions. FISH with individual BACs to flow-sorted 4C root and leaf nuclei frequently yielded more than two hybridization signals, indicating incomplete or absent sister-chromatid alignment. Up to 100% of 8C, 16C, and 32C nuclei showed no sister-chromatid alignment at defined positions. Simultaneous FISH with BACs from different chromosomal positions revealed more frequent sister-chromatid alignment in terminal than in midarm positions. Centromeric positions were mainly aligned up to a ploidy level of 16C but became separated or dispersed in 32C nuclei. DNA hypomethylation (of the whole genome) and transcriptional activity (at FWA gene position) did not impair sister-chromatid alignment. Only 6.1% of 4C leaf nuclei showed sister-chromatid separation of the entire chromosome 1 top arm territories. Homozygous transgenic tandem repeat (lac operator) arrays showing somatic homologous pairing more often than average euchromatic loci did not promote an increased frequency of sister-chromatid alignment. The high frequency of separated sister-chromatid arm positions in > or =4C nuclei suggests that sister-chromatid cohesion is variable, dynamic, and not obligatory along the entire chromosome arm in meristematic and differentiated Arabidopsis nuclei.

AB - We analyzed whether sister chromatids are continuously aligned in meristematic and endopolyploid Arabidopsis interphase nuclei by studying sister-chromatid alignment at various chromosomal positions. FISH with individual BACs to flow-sorted 4C root and leaf nuclei frequently yielded more than two hybridization signals, indicating incomplete or absent sister-chromatid alignment. Up to 100% of 8C, 16C, and 32C nuclei showed no sister-chromatid alignment at defined positions. Simultaneous FISH with BACs from different chromosomal positions revealed more frequent sister-chromatid alignment in terminal than in midarm positions. Centromeric positions were mainly aligned up to a ploidy level of 16C but became separated or dispersed in 32C nuclei. DNA hypomethylation (of the whole genome) and transcriptional activity (at FWA gene position) did not impair sister-chromatid alignment. Only 6.1% of 4C leaf nuclei showed sister-chromatid separation of the entire chromosome 1 top arm territories. Homozygous transgenic tandem repeat (lac operator) arrays showing somatic homologous pairing more often than average euchromatic loci did not promote an increased frequency of sister-chromatid alignment. The high frequency of separated sister-chromatid arm positions in > or =4C nuclei suggests that sister-chromatid cohesion is variable, dynamic, and not obligatory along the entire chromosome arm in meristematic and differentiated Arabidopsis nuclei.

U2 - 10.1534/genetics.105.048363

DO - 10.1534/genetics.105.048363

M3 - Journal article

C2 - 16157681

VL - 172

SP - 467

EP - 475

JO - Genetics

JF - Genetics

SN - 1943-2631

IS - 1

ER -

ID: 5014083