Sequence organization of barley centromeres.

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Sequence organization of barley centromeres. / Hudakova, S; Michalek, W; Presting, G G; ten Hoopen, R; dos Santos, K; Jasencakova, Zusana; Schubert, I.

In: Nucleic Acids Research, Vol. 29, No. 24, 2001, p. 5029-35.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hudakova, S, Michalek, W, Presting, GG, ten Hoopen, R, dos Santos, K, Jasencakova, Z & Schubert, I 2001, 'Sequence organization of barley centromeres.', Nucleic Acids Research, vol. 29, no. 24, pp. 5029-35.

APA

Hudakova, S., Michalek, W., Presting, G. G., ten Hoopen, R., dos Santos, K., Jasencakova, Z., & Schubert, I. (2001). Sequence organization of barley centromeres. Nucleic Acids Research, 29(24), 5029-35.

Vancouver

Hudakova S, Michalek W, Presting GG, ten Hoopen R, dos Santos K, Jasencakova Z et al. Sequence organization of barley centromeres. Nucleic Acids Research. 2001;29(24):5029-35.

Author

Hudakova, S ; Michalek, W ; Presting, G G ; ten Hoopen, R ; dos Santos, K ; Jasencakova, Zusana ; Schubert, I. / Sequence organization of barley centromeres. In: Nucleic Acids Research. 2001 ; Vol. 29, No. 24. pp. 5029-35.

Bibtex

@article{a4be1e90518b11dd8d9f000ea68e967b,
title = "Sequence organization of barley centromeres.",
abstract = "By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.",
author = "S Hudakova and W Michalek and Presting, {G G} and {ten Hoopen}, R and {dos Santos}, K and Zusana Jasencakova and I Schubert",
note = "Keywords: Blotting, Southern; Centromere; Cloning, Molecular; DNA Restriction Enzymes; DNA, Plant; Hordeum; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Mutagenesis, Insertional; Retroelements; Sequence Analysis, DNA",
year = "2001",
language = "English",
volume = "29",
pages = "5029--35",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "24",

}

RIS

TY - JOUR

T1 - Sequence organization of barley centromeres.

AU - Hudakova, S

AU - Michalek, W

AU - Presting, G G

AU - ten Hoopen, R

AU - dos Santos, K

AU - Jasencakova, Zusana

AU - Schubert, I

N1 - Keywords: Blotting, Southern; Centromere; Cloning, Molecular; DNA Restriction Enzymes; DNA, Plant; Hordeum; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Mutagenesis, Insertional; Retroelements; Sequence Analysis, DNA

PY - 2001

Y1 - 2001

N2 - By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.

AB - By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.

M3 - Journal article

C2 - 11812833

VL - 29

SP - 5029

EP - 5035

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 24

ER -

ID: 5014149