Sequence organization of barley centromeres.
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Sequence organization of barley centromeres. / Hudakova, S; Michalek, W; Presting, G G; ten Hoopen, R; dos Santos, K; Jasencakova, Zusana; Schubert, I.
In: Nucleic Acids Research, Vol. 29, No. 24, 2001, p. 5029-35.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sequence organization of barley centromeres.
AU - Hudakova, S
AU - Michalek, W
AU - Presting, G G
AU - ten Hoopen, R
AU - dos Santos, K
AU - Jasencakova, Zusana
AU - Schubert, I
N1 - Keywords: Blotting, Southern; Centromere; Cloning, Molecular; DNA Restriction Enzymes; DNA, Plant; Hordeum; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Mutagenesis, Insertional; Retroelements; Sequence Analysis, DNA
PY - 2001
Y1 - 2001
N2 - By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.
AB - By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.
M3 - Journal article
C2 - 11812833
VL - 29
SP - 5029
EP - 5035
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 24
ER -
ID: 5014149