RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells. / Østergaard, Vibe Hallundbæk; Pentzold, Constanze; Pedersen, Rune Troelsgaard; Iosif, Silviu; Alpi, Arno; Bekker-Jensen, Simon; Mailand, Niels; Lisby, Michael.

In: D N A Repair, Vol. 11, No. 11, 2012, p. 892–905.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Østergaard, VH, Pentzold, C, Pedersen, RT, Iosif, S, Alpi, A, Bekker-Jensen, S, Mailand, N & Lisby, M 2012, 'RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells', D N A Repair, vol. 11, no. 11, pp. 892–905. https://doi.org/10.1016/j.dnarep.2012.08.005

APA

Østergaard, V. H., Pentzold, C., Pedersen, R. T., Iosif, S., Alpi, A., Bekker-Jensen, S., Mailand, N., & Lisby, M. (2012). RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells. D N A Repair, 11(11), 892–905. https://doi.org/10.1016/j.dnarep.2012.08.005

Vancouver

Østergaard VH, Pentzold C, Pedersen RT, Iosif S, Alpi A, Bekker-Jensen S et al. RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells. D N A Repair. 2012;11(11):892–905. https://doi.org/10.1016/j.dnarep.2012.08.005

Author

Østergaard, Vibe Hallundbæk ; Pentzold, Constanze ; Pedersen, Rune Troelsgaard ; Iosif, Silviu ; Alpi, Arno ; Bekker-Jensen, Simon ; Mailand, Niels ; Lisby, Michael. / RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells. In: D N A Repair. 2012 ; Vol. 11, No. 11. pp. 892–905.

Bibtex

@article{5b678213e18d48ed813770ec654db898,
title = "RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells",
abstract = "The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.",
author = "{\O}stergaard, {Vibe Hallundb{\ae}k} and Constanze Pentzold and Pedersen, {Rune Troelsgaard} and Silviu Iosif and Arno Alpi and Simon Bekker-Jensen and Niels Mailand and Michael Lisby",
year = "2012",
doi = "10.1016/j.dnarep.2012.08.005",
language = "English",
volume = "11",
pages = "892–905",
journal = "DNA Repair",
issn = "1568-7864",
publisher = "Elsevier",
number = "11",

}

RIS

TY - JOUR

T1 - RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells

AU - Østergaard, Vibe Hallundbæk

AU - Pentzold, Constanze

AU - Pedersen, Rune Troelsgaard

AU - Iosif, Silviu

AU - Alpi, Arno

AU - Bekker-Jensen, Simon

AU - Mailand, Niels

AU - Lisby, Michael

PY - 2012

Y1 - 2012

N2 - The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.

AB - The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.

U2 - 10.1016/j.dnarep.2012.08.005

DO - 10.1016/j.dnarep.2012.08.005

M3 - Journal article

C2 - 23010445

VL - 11

SP - 892

EP - 905

JO - DNA Repair

JF - DNA Repair

SN - 1568-7864

IS - 11

ER -

ID: 41804581