Regulation of replication fork progression through histone supply and demand.
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Regulation of replication fork progression through histone supply and demand. / Groth, Anja; Corpet, Armelle; Cook, Adam J L; Roche, Daniele; Bartek, Jiri; Lukas, Jiri; Almouzni, Geneviève.
In: Science, Vol. 318, No. 5858, 2007, p. 1928-31.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Regulation of replication fork progression through histone supply and demand.
AU - Groth, Anja
AU - Corpet, Armelle
AU - Cook, Adam J L
AU - Roche, Daniele
AU - Bartek, Jiri
AU - Lukas, Jiri
AU - Almouzni, Geneviève
N1 - Keywords: Cell Cycle Proteins; Chromatin; DNA; DNA Replication; DNA, Single-Stranded; Hela Cells; Histones; Humans; Models, Biological; Molecular Chaperones; Nuclear Proteins; Nucleosomes; RNA Interference; S Phase
PY - 2007
Y1 - 2007
N2 - DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone chaperone Asf1 and MCM2-7, the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of Asf1 by RNA interference impedes DNA unwinding at replication sites, and similar defects arise from overproduction of new histone H3-H4 that compromises Asf1 function. These data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.
AB - DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone chaperone Asf1 and MCM2-7, the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of Asf1 by RNA interference impedes DNA unwinding at replication sites, and similar defects arise from overproduction of new histone H3-H4 that compromises Asf1 function. These data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.
U2 - 10.1126/science.1148992
DO - 10.1126/science.1148992
M3 - Journal article
C2 - 18096807
VL - 318
SP - 1928
EP - 1931
JO - Science
JF - Science
SN - 0036-8075
IS - 5858
ER -
ID: 5013946