Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit

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Standard

Refined Cryo-EM Structure of the T4 Tail Tube : Exploring the Lowest Dose Limit. / Zheng, Weili; Wang, Fengbin; Taylor, Nicholas M I; Guerrero-Ferreira, Ricardo C; Leiman, Petr G; Egelman, Edward H.

In: Structure, Vol. 25, No. 9, 05.09.2017, p. 1436-1441.e2.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zheng, W, Wang, F, Taylor, NMI, Guerrero-Ferreira, RC, Leiman, PG & Egelman, EH 2017, 'Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit', Structure, vol. 25, no. 9, pp. 1436-1441.e2. https://doi.org/10.1016/j.str.2017.06.017

APA

Zheng, W., Wang, F., Taylor, N. M. I., Guerrero-Ferreira, R. C., Leiman, P. G., & Egelman, E. H. (2017). Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit. Structure, 25(9), 1436-1441.e2. https://doi.org/10.1016/j.str.2017.06.017

Vancouver

Zheng W, Wang F, Taylor NMI, Guerrero-Ferreira RC, Leiman PG, Egelman EH. Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit. Structure. 2017 Sep 5;25(9):1436-1441.e2. https://doi.org/10.1016/j.str.2017.06.017

Author

Zheng, Weili ; Wang, Fengbin ; Taylor, Nicholas M I ; Guerrero-Ferreira, Ricardo C ; Leiman, Petr G ; Egelman, Edward H. / Refined Cryo-EM Structure of the T4 Tail Tube : Exploring the Lowest Dose Limit. In: Structure. 2017 ; Vol. 25, No. 9. pp. 1436-1441.e2.

Bibtex

@article{f4f8331a5461420b870b61f50aa35b46,
title = "Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit",
abstract = "The bacteriophage T4 contractile tail (containing a tube and sheath) was the first biological assembly reconstructed in three dimensions by electron microscopy at a resolution of ∼35 {\AA} in 1968. A single-particle reconstruction of the T4 baseplate was able to generate a 4.1 {\AA} resolution map for the first two rings of the tube using the overall baseplate for alignment. We have now reconstructed the T4 tail tube at a resolution of 3.4 {\AA}, more than a 1,000-fold increase in information content for the tube from 1968. We have used legacy software (Spider) to show that we can do better than the typical 2/3 Nyquist frequency. A reasonable map can be generated with only 1.5 electrons/{\AA}2using the higher dose images for alignment, but increasing the dose results in a better map, consistent with other reports that electron dose does not represent the main limitation on resolution in cryo-electron microscopy.",
keywords = "Bacteriophage T4/chemistry, Cryoelectron Microscopy/methods, Models, Molecular, Molecular Conformation, Software",
author = "Weili Zheng and Fengbin Wang and Taylor, {Nicholas M I} and Guerrero-Ferreira, {Ricardo C} and Leiman, {Petr G} and Egelman, {Edward H}",
note = "Copyright {\textcopyright} 2017 Elsevier Ltd. All rights reserved.",
year = "2017",
month = sep,
day = "5",
doi = "10.1016/j.str.2017.06.017",
language = "English",
volume = "25",
pages = "1436--1441.e2",
journal = "Structure",
issn = "0969-2126",
publisher = "Cell Press",
number = "9",

}

RIS

TY - JOUR

T1 - Refined Cryo-EM Structure of the T4 Tail Tube

T2 - Exploring the Lowest Dose Limit

AU - Zheng, Weili

AU - Wang, Fengbin

AU - Taylor, Nicholas M I

AU - Guerrero-Ferreira, Ricardo C

AU - Leiman, Petr G

AU - Egelman, Edward H

N1 - Copyright © 2017 Elsevier Ltd. All rights reserved.

PY - 2017/9/5

Y1 - 2017/9/5

N2 - The bacteriophage T4 contractile tail (containing a tube and sheath) was the first biological assembly reconstructed in three dimensions by electron microscopy at a resolution of ∼35 Å in 1968. A single-particle reconstruction of the T4 baseplate was able to generate a 4.1 Å resolution map for the first two rings of the tube using the overall baseplate for alignment. We have now reconstructed the T4 tail tube at a resolution of 3.4 Å, more than a 1,000-fold increase in information content for the tube from 1968. We have used legacy software (Spider) to show that we can do better than the typical 2/3 Nyquist frequency. A reasonable map can be generated with only 1.5 electrons/Å2using the higher dose images for alignment, but increasing the dose results in a better map, consistent with other reports that electron dose does not represent the main limitation on resolution in cryo-electron microscopy.

AB - The bacteriophage T4 contractile tail (containing a tube and sheath) was the first biological assembly reconstructed in three dimensions by electron microscopy at a resolution of ∼35 Å in 1968. A single-particle reconstruction of the T4 baseplate was able to generate a 4.1 Å resolution map for the first two rings of the tube using the overall baseplate for alignment. We have now reconstructed the T4 tail tube at a resolution of 3.4 Å, more than a 1,000-fold increase in information content for the tube from 1968. We have used legacy software (Spider) to show that we can do better than the typical 2/3 Nyquist frequency. A reasonable map can be generated with only 1.5 electrons/Å2using the higher dose images for alignment, but increasing the dose results in a better map, consistent with other reports that electron dose does not represent the main limitation on resolution in cryo-electron microscopy.

KW - Bacteriophage T4/chemistry

KW - Cryoelectron Microscopy/methods

KW - Models, Molecular

KW - Molecular Conformation

KW - Software

U2 - 10.1016/j.str.2017.06.017

DO - 10.1016/j.str.2017.06.017

M3 - Journal article

C2 - 28757144

VL - 25

SP - 1436-1441.e2

JO - Structure

JF - Structure

SN - 0969-2126

IS - 9

ER -

ID: 194519748