Proteomic approaches in circadian biology
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Communication
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Proteomic approaches in circadian biology. / Robles, Maria S; Mann, Matthias.
Handbook of experimental pharmacology: Circadian Clocks. 2013. p. 389-407 (Handbook of Experimental Pharmacology).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Communication
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TY - CHAP
T1 - Proteomic approaches in circadian biology
AU - Robles, Maria S
AU - Mann, Matthias
PY - 2013
Y1 - 2013
N2 - Circadian clocks are endogenous oscillators that drive the rhythmic expression of a broad array of genes that orchestrate metabolism and physiology. Recent evidence indicates that posttranscriptional and posttranslational mechanisms play essential roles in modulating circadian gene expression, particularly for the molecular mechanism of the clock. In contrast to genetic technologies that have long been used to study circadian biology, proteomic approaches have so far been limited and, if applied at all, have used two-dimensional gel electrophoresis (2-DE). Here, we review the proteomics approaches applied to date in the circadian field, and we also discuss the exciting potential of using cutting-edge proteomics technology in circadian biology. Large-scale, quantitative protein abundance measurements will help to understand to what extent the circadian clock drives system wide rhythms of protein abundance downstream of transcription regulation.
AB - Circadian clocks are endogenous oscillators that drive the rhythmic expression of a broad array of genes that orchestrate metabolism and physiology. Recent evidence indicates that posttranscriptional and posttranslational mechanisms play essential roles in modulating circadian gene expression, particularly for the molecular mechanism of the clock. In contrast to genetic technologies that have long been used to study circadian biology, proteomic approaches have so far been limited and, if applied at all, have used two-dimensional gel electrophoresis (2-DE). Here, we review the proteomics approaches applied to date in the circadian field, and we also discuss the exciting potential of using cutting-edge proteomics technology in circadian biology. Large-scale, quantitative protein abundance measurements will help to understand to what extent the circadian clock drives system wide rhythms of protein abundance downstream of transcription regulation.
KW - Animals
KW - Brain
KW - Circadian Clocks
KW - Eye
KW - Humans
KW - Metabolomics
KW - Proteomics
KW - Suprachiasmatic Nucleus
U2 - 10.1007/978-3-642-25950-0_17
DO - 10.1007/978-3-642-25950-0_17
M3 - Book chapter
C2 - 23604489
T3 - Handbook of Experimental Pharmacology
SP - 389
EP - 407
BT - Handbook of experimental pharmacology
ER -
ID: 88586811