p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes. / Fischer, Martin; Grundke, Inga; Sohr, Sindy; Quaas, Marianne; Hoffmann, Saskia; Knörck, Arne; Gumhold, Catalina; Rother, Karen.

In: PLoS ONE, Vol. 8, No. 5, 2013, p. e63187.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fischer, M, Grundke, I, Sohr, S, Quaas, M, Hoffmann, S, Knörck, A, Gumhold, C & Rother, K 2013, 'p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes', PLoS ONE, vol. 8, no. 5, pp. e63187. https://doi.org/10.1371/journal.pone.0063187

APA

Fischer, M., Grundke, I., Sohr, S., Quaas, M., Hoffmann, S., Knörck, A., Gumhold, C., & Rother, K. (2013). p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes. PLoS ONE, 8(5), e63187. https://doi.org/10.1371/journal.pone.0063187

Vancouver

Fischer M, Grundke I, Sohr S, Quaas M, Hoffmann S, Knörck A et al. p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes. PLoS ONE. 2013;8(5):e63187. https://doi.org/10.1371/journal.pone.0063187

Author

Fischer, Martin ; Grundke, Inga ; Sohr, Sindy ; Quaas, Marianne ; Hoffmann, Saskia ; Knörck, Arne ; Gumhold, Catalina ; Rother, Karen. / p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes. In: PLoS ONE. 2013 ; Vol. 8, No. 5. pp. e63187.

Bibtex

@article{f4ece9af952c404eaca3862f89dd235e,
title = "p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes",
abstract = "The microtubule-dependent molecular motor KIF23 (Kinesin family member 23) is one of two components of the centralspindlin complex assembled during late stages of mitosis. Formation of this complex is known as an essential step for cytokinesis. Here, we identified KIF23 as a new transcriptional target gene of the tumor suppressor protein p53. We showed that p53 reduces expression of KIF23 on the mRNA as well as the protein level in different cell types. Promoter reporter assays revealed that this repression results from downregulation of KIF23 promoter activity. CDK inhibitor p21(WAF1/CIP1) was shown to be necessary to mediate p53-dependent repression. Furthermore, we identified the highly conserved cell cycle genes homology region (CHR) in the KIF23 promoter to be strictly required for p53-dependent repression as well as for cell cycle-dependent expression of KIF23. Cell cycle- and p53-dependent regulation of KIF23 appeared to be controlled by differential binding of DREAM and MMB complexes to the CHR element. With this study, we describe a new mechanism for transcriptional regulation of KIF23. Considering the strongly supporting function of KIF23 in cytokinesis, its p53-dependent repression may contribute to the prevention of uncontrolled cell growth.",
keywords = "Animals, Cell Cycle, Cyclin-Dependent Kinase Inhibitor p21/metabolism, Gene Expression Regulation, Gene Silencing, HCT116 Cells, Humans, Kv Channel-Interacting Proteins/metabolism, Mice, Microtubule-Associated Proteins/genetics, NIH 3T3 Cells, Oncogene Proteins v-myb/metabolism, Promoter Regions, Genetic, Protein Binding, Protein Isoforms/genetics, Protein Stability, RNA, Messenger/genetics, Repressor Proteins/metabolism, Response Elements, Transcription, Genetic, Tumor Suppressor Protein p53/metabolism",
author = "Martin Fischer and Inga Grundke and Sindy Sohr and Marianne Quaas and Saskia Hoffmann and Arne Kn{\"o}rck and Catalina Gumhold and Karen Rother",
year = "2013",
doi = "10.1371/journal.pone.0063187",
language = "English",
volume = "8",
pages = "e63187",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS

TY - JOUR

T1 - p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes

AU - Fischer, Martin

AU - Grundke, Inga

AU - Sohr, Sindy

AU - Quaas, Marianne

AU - Hoffmann, Saskia

AU - Knörck, Arne

AU - Gumhold, Catalina

AU - Rother, Karen

PY - 2013

Y1 - 2013

N2 - The microtubule-dependent molecular motor KIF23 (Kinesin family member 23) is one of two components of the centralspindlin complex assembled during late stages of mitosis. Formation of this complex is known as an essential step for cytokinesis. Here, we identified KIF23 as a new transcriptional target gene of the tumor suppressor protein p53. We showed that p53 reduces expression of KIF23 on the mRNA as well as the protein level in different cell types. Promoter reporter assays revealed that this repression results from downregulation of KIF23 promoter activity. CDK inhibitor p21(WAF1/CIP1) was shown to be necessary to mediate p53-dependent repression. Furthermore, we identified the highly conserved cell cycle genes homology region (CHR) in the KIF23 promoter to be strictly required for p53-dependent repression as well as for cell cycle-dependent expression of KIF23. Cell cycle- and p53-dependent regulation of KIF23 appeared to be controlled by differential binding of DREAM and MMB complexes to the CHR element. With this study, we describe a new mechanism for transcriptional regulation of KIF23. Considering the strongly supporting function of KIF23 in cytokinesis, its p53-dependent repression may contribute to the prevention of uncontrolled cell growth.

AB - The microtubule-dependent molecular motor KIF23 (Kinesin family member 23) is one of two components of the centralspindlin complex assembled during late stages of mitosis. Formation of this complex is known as an essential step for cytokinesis. Here, we identified KIF23 as a new transcriptional target gene of the tumor suppressor protein p53. We showed that p53 reduces expression of KIF23 on the mRNA as well as the protein level in different cell types. Promoter reporter assays revealed that this repression results from downregulation of KIF23 promoter activity. CDK inhibitor p21(WAF1/CIP1) was shown to be necessary to mediate p53-dependent repression. Furthermore, we identified the highly conserved cell cycle genes homology region (CHR) in the KIF23 promoter to be strictly required for p53-dependent repression as well as for cell cycle-dependent expression of KIF23. Cell cycle- and p53-dependent regulation of KIF23 appeared to be controlled by differential binding of DREAM and MMB complexes to the CHR element. With this study, we describe a new mechanism for transcriptional regulation of KIF23. Considering the strongly supporting function of KIF23 in cytokinesis, its p53-dependent repression may contribute to the prevention of uncontrolled cell growth.

KW - Animals

KW - Cell Cycle

KW - Cyclin-Dependent Kinase Inhibitor p21/metabolism

KW - Gene Expression Regulation

KW - Gene Silencing

KW - HCT116 Cells

KW - Humans

KW - Kv Channel-Interacting Proteins/metabolism

KW - Mice

KW - Microtubule-Associated Proteins/genetics

KW - NIH 3T3 Cells

KW - Oncogene Proteins v-myb/metabolism

KW - Promoter Regions, Genetic

KW - Protein Binding

KW - Protein Isoforms/genetics

KW - Protein Stability

KW - RNA, Messenger/genetics

KW - Repressor Proteins/metabolism

KW - Response Elements

KW - Transcription, Genetic

KW - Tumor Suppressor Protein p53/metabolism

U2 - 10.1371/journal.pone.0063187

DO - 10.1371/journal.pone.0063187

M3 - Journal article

C2 - 23650552

VL - 8

SP - e63187

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 5

ER -

ID: 237849703