p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. / Tollenaere, Maxim A X; Villumsen, Bine H; Blasius, Melanie; Nielsen, Julie C; Wagner, Sebastian A; Bartek, Jiri; Beli, Petra; Mailand, Niels; Bekker-Jensen, Simon.

In: Nature Communications, Vol. 6, 10075, 30.11.2015, p. 1-12.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tollenaere, MAX, Villumsen, BH, Blasius, M, Nielsen, JC, Wagner, SA, Bartek, J, Beli, P, Mailand, N & Bekker-Jensen, S 2015, 'p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1', Nature Communications, vol. 6, 10075, pp. 1-12. https://doi.org/10.1038/ncomms10075

APA

Tollenaere, M. A. X., Villumsen, B. H., Blasius, M., Nielsen, J. C., Wagner, S. A., Bartek, J., Beli, P., Mailand, N., & Bekker-Jensen, S. (2015). p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nature Communications, 6, 1-12. [10075]. https://doi.org/10.1038/ncomms10075

Vancouver

Tollenaere MAX, Villumsen BH, Blasius M, Nielsen JC, Wagner SA, Bartek J et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nature Communications. 2015 Nov 30;6:1-12. 10075. https://doi.org/10.1038/ncomms10075

Author

Tollenaere, Maxim A X ; Villumsen, Bine H ; Blasius, Melanie ; Nielsen, Julie C ; Wagner, Sebastian A ; Bartek, Jiri ; Beli, Petra ; Mailand, Niels ; Bekker-Jensen, Simon. / p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. In: Nature Communications. 2015 ; Vol. 6. pp. 1-12.

Bibtex

@article{717ca35f95f34e49a2c87e8ae8f1e78a,
title = "p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1",
abstract = "Centriolar satellites (CS) are small granular structures that cluster in the vicinity of centrosomes. CS are highly susceptible to stress stimuli, triggering abrupt displacement of key CS factors. Here we discover a linear p38-MK2-14-3-3 signalling pathway that specifically targets CEP131 to trigger CS remodelling after cell stress. We identify CEP131 as a substrate of the p38 effector kinase MK2 and pinpoint S47 and S78 as critical MK2 phosphorylation sites in CEP131. Ultraviolet-induced phosphorylation of these residues generates direct binding sites for 14-3-3 proteins, which sequester CEP131 in the cytoplasm to block formation of new CS, thereby leading to rapid depletion of these structures. Mutating S47 and S78 in CEP131 is sufficient to abolish stress-induced CS reorganization, demonstrating that CEP131 is the key regulatory target of MK2 and 14-3-3 in these structures. Our findings reveal the molecular mechanism underlying dynamic CS remodelling to modulate centrosome functions on cell stress.",
author = "Tollenaere, {Maxim A X} and Villumsen, {Bine H} and Melanie Blasius and Nielsen, {Julie C} and Wagner, {Sebastian A} and Jiri Bartek and Petra Beli and Niels Mailand and Simon Bekker-Jensen",
year = "2015",
month = nov,
day = "30",
doi = "10.1038/ncomms10075",
language = "English",
volume = "6",
pages = "1--12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1

AU - Tollenaere, Maxim A X

AU - Villumsen, Bine H

AU - Blasius, Melanie

AU - Nielsen, Julie C

AU - Wagner, Sebastian A

AU - Bartek, Jiri

AU - Beli, Petra

AU - Mailand, Niels

AU - Bekker-Jensen, Simon

PY - 2015/11/30

Y1 - 2015/11/30

N2 - Centriolar satellites (CS) are small granular structures that cluster in the vicinity of centrosomes. CS are highly susceptible to stress stimuli, triggering abrupt displacement of key CS factors. Here we discover a linear p38-MK2-14-3-3 signalling pathway that specifically targets CEP131 to trigger CS remodelling after cell stress. We identify CEP131 as a substrate of the p38 effector kinase MK2 and pinpoint S47 and S78 as critical MK2 phosphorylation sites in CEP131. Ultraviolet-induced phosphorylation of these residues generates direct binding sites for 14-3-3 proteins, which sequester CEP131 in the cytoplasm to block formation of new CS, thereby leading to rapid depletion of these structures. Mutating S47 and S78 in CEP131 is sufficient to abolish stress-induced CS reorganization, demonstrating that CEP131 is the key regulatory target of MK2 and 14-3-3 in these structures. Our findings reveal the molecular mechanism underlying dynamic CS remodelling to modulate centrosome functions on cell stress.

AB - Centriolar satellites (CS) are small granular structures that cluster in the vicinity of centrosomes. CS are highly susceptible to stress stimuli, triggering abrupt displacement of key CS factors. Here we discover a linear p38-MK2-14-3-3 signalling pathway that specifically targets CEP131 to trigger CS remodelling after cell stress. We identify CEP131 as a substrate of the p38 effector kinase MK2 and pinpoint S47 and S78 as critical MK2 phosphorylation sites in CEP131. Ultraviolet-induced phosphorylation of these residues generates direct binding sites for 14-3-3 proteins, which sequester CEP131 in the cytoplasm to block formation of new CS, thereby leading to rapid depletion of these structures. Mutating S47 and S78 in CEP131 is sufficient to abolish stress-induced CS reorganization, demonstrating that CEP131 is the key regulatory target of MK2 and 14-3-3 in these structures. Our findings reveal the molecular mechanism underlying dynamic CS remodelling to modulate centrosome functions on cell stress.

U2 - 10.1038/ncomms10075

DO - 10.1038/ncomms10075

M3 - Journal article

C2 - 26616734

VL - 6

SP - 1

EP - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 10075

ER -

ID: 152243599