One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes: [Inkl. Correction]
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One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes : [Inkl. Correction]. / Ye, Zilu; Sabatier, Pierre; Martin-Gonzalez, Javier; Eguchi, Akihiro; Lechner, Maico; Østergaard, Ole; Xie, Jingsheng; Guo, Yuan; Schultz, Lesley; Truffer, Rafaela; Bekker-Jensen, Dorte B.; Bache, Nicolai; Olsen, Jesper V.
In: Nature Communications, Vol. 15, No. 1, 2474, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
T2 - [Inkl. Correction]
AU - Ye, Zilu
AU - Sabatier, Pierre
AU - Martin-Gonzalez, Javier
AU - Eguchi, Akihiro
AU - Lechner, Maico
AU - Østergaard, Ole
AU - Xie, Jingsheng
AU - Guo, Yuan
AU - Schultz, Lesley
AU - Truffer, Rafaela
AU - Bekker-Jensen, Dorte B.
AU - Bache, Nicolai
AU - Olsen, Jesper V.
N1 - Correction: 10.1038/s41467-024-47496-x Link: https://www.nature.com/articles/s41467-024-47496-x
PY - 2024
Y1 - 2024
N2 - Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types.
AB - Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types.
U2 - 10.1038/s41467-024-46777-9
DO - 10.1038/s41467-024-46777-9
M3 - Journal article
C2 - 38503780
AN - SCOPUS:85188057575
VL - 15
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 2474
ER -
ID: 387020946