Molecular scissors for in situ cellular repair

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Molecular scissors for in situ cellular repair. / Prieto, Jesús; Molina, Rafael; Montoya, Guillermo.

In: Critical Reviews in Biochemistry and Molecular Biology, Vol. 47, No. 3, 31.01.2012, p. 207-21.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Prieto, J, Molina, R & Montoya, G 2012, 'Molecular scissors for in situ cellular repair', Critical Reviews in Biochemistry and Molecular Biology, vol. 47, no. 3, pp. 207-21. https://doi.org/10.3109/10409238.2011.652358

APA

Prieto, J., Molina, R., & Montoya, G. (2012). Molecular scissors for in situ cellular repair. Critical Reviews in Biochemistry and Molecular Biology, 47(3), 207-21. https://doi.org/10.3109/10409238.2011.652358

Vancouver

Prieto J, Molina R, Montoya G. Molecular scissors for in situ cellular repair. Critical Reviews in Biochemistry and Molecular Biology. 2012 Jan 31;47(3):207-21. https://doi.org/10.3109/10409238.2011.652358

Author

Prieto, Jesús ; Molina, Rafael ; Montoya, Guillermo. / Molecular scissors for in situ cellular repair. In: Critical Reviews in Biochemistry and Molecular Biology. 2012 ; Vol. 47, No. 3. pp. 207-21.

Bibtex

@article{9d1a3b1488b742488737970bdb87cb8a,
title = "Molecular scissors for in situ cellular repair",
abstract = "The engineering of protein-DNA interactions in different protein scaffolds may provide {"}toolkits{"} to modify the genome. Homing endonucleases are powerful tools for genome manipulation through homologous recombination, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. Therefore, the combination of a precise {"}cutter{"} with the presence of a natural or modified homologous DNA donor provides a potentially useful means to modify the genome. However, the basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. The engineering of homing endonucleases and alternative scaffolds, such as zinc fingers or transcription activator-like effector domains, has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Customized homing endonucleases targeting selected human genes can excise or correct regions of genes implicated in monogenic diseases, thereby representing important tools for intervention in eukaryotic genomes.",
keywords = "DNA Cleavage, DNA Repair, Endonucleases/genetics, Genetic Diseases, Inborn/therapy, Genetic Therapy/methods, Genetic Vectors, Genome, Human, Humans, Protein Engineering/methods, Protein Structure, Tertiary, Substrate Specificity, Zinc Fingers",
author = "Jes{\'u}s Prieto and Rafael Molina and Guillermo Montoya",
year = "2012",
month = jan,
day = "31",
doi = "10.3109/10409238.2011.652358",
language = "English",
volume = "47",
pages = "207--21",
journal = "Critical Reviews in Biochemistry and Molecular Biology",
issn = "1040-9238",
publisher = "Taylor & Francis",
number = "3",

}

RIS

TY - JOUR

T1 - Molecular scissors for in situ cellular repair

AU - Prieto, Jesús

AU - Molina, Rafael

AU - Montoya, Guillermo

PY - 2012/1/31

Y1 - 2012/1/31

N2 - The engineering of protein-DNA interactions in different protein scaffolds may provide "toolkits" to modify the genome. Homing endonucleases are powerful tools for genome manipulation through homologous recombination, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. Therefore, the combination of a precise "cutter" with the presence of a natural or modified homologous DNA donor provides a potentially useful means to modify the genome. However, the basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. The engineering of homing endonucleases and alternative scaffolds, such as zinc fingers or transcription activator-like effector domains, has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Customized homing endonucleases targeting selected human genes can excise or correct regions of genes implicated in monogenic diseases, thereby representing important tools for intervention in eukaryotic genomes.

AB - The engineering of protein-DNA interactions in different protein scaffolds may provide "toolkits" to modify the genome. Homing endonucleases are powerful tools for genome manipulation through homologous recombination, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. Therefore, the combination of a precise "cutter" with the presence of a natural or modified homologous DNA donor provides a potentially useful means to modify the genome. However, the basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. The engineering of homing endonucleases and alternative scaffolds, such as zinc fingers or transcription activator-like effector domains, has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Customized homing endonucleases targeting selected human genes can excise or correct regions of genes implicated in monogenic diseases, thereby representing important tools for intervention in eukaryotic genomes.

KW - DNA Cleavage

KW - DNA Repair

KW - Endonucleases/genetics

KW - Genetic Diseases, Inborn/therapy

KW - Genetic Therapy/methods

KW - Genetic Vectors

KW - Genome, Human

KW - Humans

KW - Protein Engineering/methods

KW - Protein Structure, Tertiary

KW - Substrate Specificity

KW - Zinc Fingers

U2 - 10.3109/10409238.2011.652358

DO - 10.3109/10409238.2011.652358

M3 - Review

C2 - 22283548

VL - 47

SP - 207

EP - 221

JO - Critical Reviews in Biochemistry and Molecular Biology

JF - Critical Reviews in Biochemistry and Molecular Biology

SN - 1040-9238

IS - 3

ER -

ID: 203018961