Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC

Research output: Contribution to journal › Journal article › Research › peer-review

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Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC. / Frey, Felice; Sheahan, Thomas; Finkl, Katja; Stoehr, Gabriele; Mann, Matthias; Benda, Christian; Müller, Jürg.

In: Genes & Development, Vol. 30, No. 9, 01.05.2016, p. 1116-27.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Frey, F, Sheahan, T, Finkl, K, Stoehr, G, Mann, M, Benda, C & Müller, J 2016, 'Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC', Genes & Development, vol. 30, no. 9, pp. 1116-27. https://doi.org/10.1101/gad.279141.116

APA

Frey, F., Sheahan, T., Finkl, K., Stoehr, G., Mann, M., Benda, C., & Müller, J. (2016). Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC. Genes & Development, 30(9), 1116-27. https://doi.org/10.1101/gad.279141.116

Vancouver

Frey F, Sheahan T, Finkl K, Stoehr G, Mann M, Benda C et al. Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC. Genes & Development. 2016 May 1;30(9):1116-27. https://doi.org/10.1101/gad.279141.116

Author

Frey, Felice ; Sheahan, Thomas ; Finkl, Katja ; Stoehr, Gabriele ; Mann, Matthias ; Benda, Christian ; Müller, Jürg. / Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC. In: Genes & Development. 2016 ; Vol. 30, No. 9. pp. 1116-27.

Bibtex

@article{bb6e7ea08ee4472fad7ba5c3d0a163d4,
title = "Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC",
abstract = "Polycomb group (PcG) protein complexes repress transcription by modifying target gene chromatin. In Drosophila, this repression requires association of PcG protein complexes with cis-regulatory Polycomb response elements (PREs), but the interactions permitting formation of these assemblies are poorly understood. We show that the Sfmbt subunit of the DNA-binding Pho-repressive complex (PhoRC) and the Scm subunit of the canonical Polycomb-repressive complex 1 (PRC1) directly bind each other through their SAM domains. The 1.9 {\AA} crystal structure of the Scm-SAM:Sfmbt-SAM complex reveals the recognition mechanism and shows that Sfmbt-SAM lacks the polymerization capacity of the SAM domains of Scm and its PRC1 partner subunit, Ph. Functional analyses in Drosophila demonstrate that Sfmbt-SAM and Scm-SAM are essential for repression and that PhoRC DNA binding is critical to initiate PRC1 association with PREs. Together, this suggests that PRE-tethered Sfmbt-SAM nucleates PRC1 recruitment and that Scm-SAM/Ph-SAM-mediated polymerization then results in the formation of PRC1-compacted chromatin.",
keywords = "Animals, Chromatin, Crystallography, Drosophila Proteins, Drosophila melanogaster, Gene Expression Regulation, Models, Molecular, Polycomb Repressive Complex 1, Polycomb-Group Proteins, Polymerization, Protein Binding, Protein Structure, Tertiary, Response Elements, Journal Article",
author = "Felice Frey and Thomas Sheahan and Katja Finkl and Gabriele Stoehr and Matthias Mann and Christian Benda and J{\"u}rg M{\"u}ller",
note = "{\textcopyright} 2016 Frey et al.; Published by Cold Spring Harbor Laboratory Press.",
year = "2016",
month = may,
day = "1",
doi = "10.1101/gad.279141.116",
language = "English",
volume = "30",
pages = "1116--27",
journal = "Genes & Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "9",

}

RIS

TY - JOUR

T1 - Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC

AU - Frey, Felice

AU - Sheahan, Thomas

AU - Finkl, Katja

AU - Stoehr, Gabriele

AU - Mann, Matthias

AU - Benda, Christian

AU - Müller, Jürg

N1 - © 2016 Frey et al.; Published by Cold Spring Harbor Laboratory Press.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Polycomb group (PcG) protein complexes repress transcription by modifying target gene chromatin. In Drosophila, this repression requires association of PcG protein complexes with cis-regulatory Polycomb response elements (PREs), but the interactions permitting formation of these assemblies are poorly understood. We show that the Sfmbt subunit of the DNA-binding Pho-repressive complex (PhoRC) and the Scm subunit of the canonical Polycomb-repressive complex 1 (PRC1) directly bind each other through their SAM domains. The 1.9 Å crystal structure of the Scm-SAM:Sfmbt-SAM complex reveals the recognition mechanism and shows that Sfmbt-SAM lacks the polymerization capacity of the SAM domains of Scm and its PRC1 partner subunit, Ph. Functional analyses in Drosophila demonstrate that Sfmbt-SAM and Scm-SAM are essential for repression and that PhoRC DNA binding is critical to initiate PRC1 association with PREs. Together, this suggests that PRE-tethered Sfmbt-SAM nucleates PRC1 recruitment and that Scm-SAM/Ph-SAM-mediated polymerization then results in the formation of PRC1-compacted chromatin.

AB - Polycomb group (PcG) protein complexes repress transcription by modifying target gene chromatin. In Drosophila, this repression requires association of PcG protein complexes with cis-regulatory Polycomb response elements (PREs), but the interactions permitting formation of these assemblies are poorly understood. We show that the Sfmbt subunit of the DNA-binding Pho-repressive complex (PhoRC) and the Scm subunit of the canonical Polycomb-repressive complex 1 (PRC1) directly bind each other through their SAM domains. The 1.9 Å crystal structure of the Scm-SAM:Sfmbt-SAM complex reveals the recognition mechanism and shows that Sfmbt-SAM lacks the polymerization capacity of the SAM domains of Scm and its PRC1 partner subunit, Ph. Functional analyses in Drosophila demonstrate that Sfmbt-SAM and Scm-SAM are essential for repression and that PhoRC DNA binding is critical to initiate PRC1 association with PREs. Together, this suggests that PRE-tethered Sfmbt-SAM nucleates PRC1 recruitment and that Scm-SAM/Ph-SAM-mediated polymerization then results in the formation of PRC1-compacted chromatin.

KW - Animals

KW - Chromatin

KW - Crystallography

KW - Drosophila Proteins

KW - Drosophila melanogaster

KW - Gene Expression Regulation

KW - Models, Molecular

KW - Polycomb Repressive Complex 1

KW - Polycomb-Group Proteins

KW - Polymerization

KW - Protein Binding

KW - Protein Structure, Tertiary

KW - Response Elements

KW - Journal Article

U2 - 10.1101/gad.279141.116

DO - 10.1101/gad.279141.116

M3 - Journal article

C2 - 27151979

VL - 30

SP - 1116

EP - 1127

JO - Genes & Development

JF - Genes & Development

SN - 0890-9369

IS - 9

ER -

ID: 186876710