Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Membrane Protein Cryo-EM : Cryo-Grid Optimization and Data Collection with Protein in Detergent. / Bloch, Magnus; Santiveri, Mònica; Taylor, Nicholas M.I.

Expression, Purification, and Structural Biology of Membrane Proteins. ed. / Camilo Perez; Timm Maier. Humana Press, 2020. p. 227-244 (Methods in Molecular Biology, Vol. 2127).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Bloch, M, Santiveri, M & Taylor, NMI 2020, Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent. in C Perez & T Maier (eds), Expression, Purification, and Structural Biology of Membrane Proteins. Humana Press, Methods in Molecular Biology, vol. 2127, pp. 227-244. https://doi.org/10.1007/978-1-0716-0373-4_16

APA

Bloch, M., Santiveri, M., & Taylor, N. M. I. (2020). Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent. In C. Perez, & T. Maier (Eds.), Expression, Purification, and Structural Biology of Membrane Proteins (pp. 227-244). Humana Press. Methods in Molecular Biology Vol. 2127 https://doi.org/10.1007/978-1-0716-0373-4_16

Vancouver

Bloch M, Santiveri M, Taylor NMI. Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent. In Perez C, Maier T, editors, Expression, Purification, and Structural Biology of Membrane Proteins. Humana Press. 2020. p. 227-244. (Methods in Molecular Biology, Vol. 2127). https://doi.org/10.1007/978-1-0716-0373-4_16

Author

Bloch, Magnus ; Santiveri, Mònica ; Taylor, Nicholas M.I. / Membrane Protein Cryo-EM : Cryo-Grid Optimization and Data Collection with Protein in Detergent. Expression, Purification, and Structural Biology of Membrane Proteins. editor / Camilo Perez ; Timm Maier. Humana Press, 2020. pp. 227-244 (Methods in Molecular Biology, Vol. 2127).

Bibtex

@inbook{e7a4db71e44b4516b842f52e66a003fd,
title = "Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent",
abstract = "Cryo-electron microscopy (cryo-EM) is a powerful tool for investigating the structure of macromolecules under near-native conditions. Especially in the context of membrane proteins, this technique has allowed researchers to obtain structural information at a previously unattainable level of detail. Specimen preparation remains the bottleneck of most cryo-EM research projects, with membrane proteins representing particularly challenging targets of investigation due to their universal requirement for detergents or other solubilizing agents. Here we describe preparation of negative staining and cryo-EM grids and downstream data collection of membrane proteins in detergent, by far the most common solubilization agent. This protocol outlines a quick and straightforward procedure for screening and determining the structure of a membrane protein of interest under biologically relevant conditions.",
keywords = "Cryo-electron microscopy, Data collection, Detergent, Grid preparation, Membrane protein, Negative staining, Structural biology, Vitrification",
author = "Magnus Bloch and M{\`o}nica Santiveri and Taylor, {Nicholas M.I.}",
year = "2020",
doi = "10.1007/978-1-0716-0373-4_16",
language = "English",
isbn = "978-1-0716-0372-7",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "227--244",
editor = "Camilo Perez and Timm Maier",
booktitle = "Expression, Purification, and Structural Biology of Membrane Proteins",
address = "United States",

}

RIS

TY - CHAP

T1 - Membrane Protein Cryo-EM

T2 - Cryo-Grid Optimization and Data Collection with Protein in Detergent

AU - Bloch, Magnus

AU - Santiveri, Mònica

AU - Taylor, Nicholas M.I.

PY - 2020

Y1 - 2020

N2 - Cryo-electron microscopy (cryo-EM) is a powerful tool for investigating the structure of macromolecules under near-native conditions. Especially in the context of membrane proteins, this technique has allowed researchers to obtain structural information at a previously unattainable level of detail. Specimen preparation remains the bottleneck of most cryo-EM research projects, with membrane proteins representing particularly challenging targets of investigation due to their universal requirement for detergents or other solubilizing agents. Here we describe preparation of negative staining and cryo-EM grids and downstream data collection of membrane proteins in detergent, by far the most common solubilization agent. This protocol outlines a quick and straightforward procedure for screening and determining the structure of a membrane protein of interest under biologically relevant conditions.

AB - Cryo-electron microscopy (cryo-EM) is a powerful tool for investigating the structure of macromolecules under near-native conditions. Especially in the context of membrane proteins, this technique has allowed researchers to obtain structural information at a previously unattainable level of detail. Specimen preparation remains the bottleneck of most cryo-EM research projects, with membrane proteins representing particularly challenging targets of investigation due to their universal requirement for detergents or other solubilizing agents. Here we describe preparation of negative staining and cryo-EM grids and downstream data collection of membrane proteins in detergent, by far the most common solubilization agent. This protocol outlines a quick and straightforward procedure for screening and determining the structure of a membrane protein of interest under biologically relevant conditions.

KW - Cryo-electron microscopy

KW - Data collection

KW - Detergent

KW - Grid preparation

KW - Membrane protein

KW - Negative staining

KW - Structural biology

KW - Vitrification

U2 - 10.1007/978-1-0716-0373-4_16

DO - 10.1007/978-1-0716-0373-4_16

M3 - Book chapter

C2 - 32112326

AN - SCOPUS:85080912616

SN - 978-1-0716-0372-7

T3 - Methods in Molecular Biology

SP - 227

EP - 244

BT - Expression, Purification, and Structural Biology of Membrane Proteins

A2 - Perez, Camilo

A2 - Maier, Timm

PB - Humana Press

ER -

ID: 238002304