Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in Arabidopsis
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Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in Arabidopsis. / Hartl, Markus; Füßl, Magdalena; Boersema, Paul J.; Jost, Jan-Oliver; Kramer, Katharina; Bakirbas, Ahmet; Sindlinger, Julia; Plöchinger, Magdalena; Leister, Dario; Uhrig, Glen; Moorhead, Greg Bg; Cox, Jürgen; Salvucci, Michael E; Schwarzer, Dirk; Mann, Matthias; Finkemeier, Iris.
In: Molecular Systems Biology, Vol. 13, No. 10, 949, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in Arabidopsis
AU - Hartl, Markus
AU - Füßl, Magdalena
AU - Boersema, Paul J.
AU - Jost, Jan-Oliver
AU - Kramer, Katharina
AU - Bakirbas, Ahmet
AU - Sindlinger, Julia
AU - Plöchinger, Magdalena
AU - Leister, Dario
AU - Uhrig, Glen
AU - Moorhead, Greg Bg
AU - Cox, Jürgen
AU - Salvucci, Michael E
AU - Schwarzer, Dirk
AU - Mann, Matthias
AU - Finkemeier, Iris
N1 - © 2017 The Authors. Published under the terms of the CC BY 4.0 license.
PY - 2017
Y1 - 2017
N2 - Histone deacetylases have central functions in regulating stress defenses and development in plants. However, the knowledge about the deacetylase functions is largely limited to histones, although these enzymes were found in diverse subcellular compartments. In this study, we determined the proteome-wide signatures of the RPD3/HDA1 class of histone deacetylases in Arabidopsis Relative quantification of the changes in the lysine acetylation levels was determined on a proteome-wide scale after treatment of Arabidopsis leaves with deacetylase inhibitors apicidin and trichostatin A. We identified 91 new acetylated candidate proteins other than histones, which are potential substrates of the RPD3/HDA1-like histone deacetylases in Arabidopsis, of which at least 30 of these proteins function in nucleic acid binding. Furthermore, our analysis revealed that histone deacetylase 14 (HDA14) is the first organellar-localized RPD3/HDA1 class protein found to reside in the chloroplasts and that the majority of its protein targets have functions in photosynthesis. Finally, the analysis of HDA14 loss-of-function mutants revealed that the activation state of RuBisCO is controlled by lysine acetylation of RuBisCO activase under low-light conditions.
AB - Histone deacetylases have central functions in regulating stress defenses and development in plants. However, the knowledge about the deacetylase functions is largely limited to histones, although these enzymes were found in diverse subcellular compartments. In this study, we determined the proteome-wide signatures of the RPD3/HDA1 class of histone deacetylases in Arabidopsis Relative quantification of the changes in the lysine acetylation levels was determined on a proteome-wide scale after treatment of Arabidopsis leaves with deacetylase inhibitors apicidin and trichostatin A. We identified 91 new acetylated candidate proteins other than histones, which are potential substrates of the RPD3/HDA1-like histone deacetylases in Arabidopsis, of which at least 30 of these proteins function in nucleic acid binding. Furthermore, our analysis revealed that histone deacetylase 14 (HDA14) is the first organellar-localized RPD3/HDA1 class protein found to reside in the chloroplasts and that the majority of its protein targets have functions in photosynthesis. Finally, the analysis of HDA14 loss-of-function mutants revealed that the activation state of RuBisCO is controlled by lysine acetylation of RuBisCO activase under low-light conditions.
KW - Journal Article
U2 - 10.15252/msb.20177819
DO - 10.15252/msb.20177819
M3 - Journal article
C2 - 29061669
VL - 13
JO - Molecular Systems Biology
JF - Molecular Systems Biology
SN - 1744-4292
IS - 10
M1 - 949
ER -
ID: 186184736