Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis. / Løvendorf, Marianne B; Mitsui, Hiroshi; Zibert, John R; Røpke, Mads A; Hafner, Markus; Dyring-Andersen, Beatrice; Bonefeld, Charlotte M; Krueger, James G; Skov, Lone.

In: Experimental Dermatology, Vol. 24, No. 3, 03.2015, p. 187-93.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Løvendorf, MB, Mitsui, H, Zibert, JR, Røpke, MA, Hafner, M, Dyring-Andersen, B, Bonefeld, CM, Krueger, JG & Skov, L 2015, 'Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis', Experimental Dermatology, vol. 24, no. 3, pp. 187-93. https://doi.org/10.1111/exd.12604

APA

Løvendorf, M. B., Mitsui, H., Zibert, J. R., Røpke, M. A., Hafner, M., Dyring-Andersen, B., Bonefeld, C. M., Krueger, J. G., & Skov, L. (2015). Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis. Experimental Dermatology, 24(3), 187-93. https://doi.org/10.1111/exd.12604

Vancouver

Løvendorf MB, Mitsui H, Zibert JR, Røpke MA, Hafner M, Dyring-Andersen B et al. Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis. Experimental Dermatology. 2015 Mar;24(3):187-93. https://doi.org/10.1111/exd.12604

Author

Løvendorf, Marianne B ; Mitsui, Hiroshi ; Zibert, John R ; Røpke, Mads A ; Hafner, Markus ; Dyring-Andersen, Beatrice ; Bonefeld, Charlotte M ; Krueger, James G ; Skov, Lone. / Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis. In: Experimental Dermatology. 2015 ; Vol. 24, No. 3. pp. 187-93.

Bibtex

@article{b7044302f778421c9fcadc09cda8ce45,
title = "Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis",
abstract = "Psoriasis is a systemic disease with cutaneous manifestations. MicroRNAs (miRNAs) are small non-coding RNA molecules that are differentially expressed in psoriatic skin; however, only few cell- and region-specific miRNAs have been identified in psoriatic lesions. We used laser capture microdissection (LCM) and next-generation sequencing (NGS) to study the specific miRNA expression profiles in the epidermis (Epi) and dermal inflammatory infiltrates (RD) of psoriatic skin (N = 6). We identified 24 deregulated miRNAs in the Epi and 37 deregulated miRNAs in the RD of psoriatic plaque compared with normal psoriatic skin (FCH > 2, FDR < 0.05). Interestingly, 9 of the 37 miRNAs in RD, including miR-193b and miR-223, were recently described as deregulated in circulating peripheral blood mononuclear cells (PBMCs) from patients with psoriasis. Using flow cytometry and qRT-PCR, we found that miR-193b and miR-223 were expressed in Th17 cells. In conclusion, we demonstrate that LCM combined with NGS provides a robust approach to explore the global miRNA expression in the epidermal and dermal compartments of psoriatic skin. Furthermore, our results indicate that the altered local miRNA changes seen in the RD are reflected in the circulating immune cells, suggesting that miRNAs may contribute to the pathogenesis of psoriasis.",
author = "L{\o}vendorf, {Marianne B} and Hiroshi Mitsui and Zibert, {John R} and R{\o}pke, {Mads A} and Markus Hafner and Beatrice Dyring-Andersen and Bonefeld, {Charlotte M} and Krueger, {James G} and Lone Skov",
note = "{\textcopyright} 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",
year = "2015",
month = mar,
doi = "10.1111/exd.12604",
language = "English",
volume = "24",
pages = "187--93",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that reflect systemic microRNA changes in psoriasis

AU - Løvendorf, Marianne B

AU - Mitsui, Hiroshi

AU - Zibert, John R

AU - Røpke, Mads A

AU - Hafner, Markus

AU - Dyring-Andersen, Beatrice

AU - Bonefeld, Charlotte M

AU - Krueger, James G

AU - Skov, Lone

N1 - © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PY - 2015/3

Y1 - 2015/3

N2 - Psoriasis is a systemic disease with cutaneous manifestations. MicroRNAs (miRNAs) are small non-coding RNA molecules that are differentially expressed in psoriatic skin; however, only few cell- and region-specific miRNAs have been identified in psoriatic lesions. We used laser capture microdissection (LCM) and next-generation sequencing (NGS) to study the specific miRNA expression profiles in the epidermis (Epi) and dermal inflammatory infiltrates (RD) of psoriatic skin (N = 6). We identified 24 deregulated miRNAs in the Epi and 37 deregulated miRNAs in the RD of psoriatic plaque compared with normal psoriatic skin (FCH > 2, FDR < 0.05). Interestingly, 9 of the 37 miRNAs in RD, including miR-193b and miR-223, were recently described as deregulated in circulating peripheral blood mononuclear cells (PBMCs) from patients with psoriasis. Using flow cytometry and qRT-PCR, we found that miR-193b and miR-223 were expressed in Th17 cells. In conclusion, we demonstrate that LCM combined with NGS provides a robust approach to explore the global miRNA expression in the epidermal and dermal compartments of psoriatic skin. Furthermore, our results indicate that the altered local miRNA changes seen in the RD are reflected in the circulating immune cells, suggesting that miRNAs may contribute to the pathogenesis of psoriasis.

AB - Psoriasis is a systemic disease with cutaneous manifestations. MicroRNAs (miRNAs) are small non-coding RNA molecules that are differentially expressed in psoriatic skin; however, only few cell- and region-specific miRNAs have been identified in psoriatic lesions. We used laser capture microdissection (LCM) and next-generation sequencing (NGS) to study the specific miRNA expression profiles in the epidermis (Epi) and dermal inflammatory infiltrates (RD) of psoriatic skin (N = 6). We identified 24 deregulated miRNAs in the Epi and 37 deregulated miRNAs in the RD of psoriatic plaque compared with normal psoriatic skin (FCH > 2, FDR < 0.05). Interestingly, 9 of the 37 miRNAs in RD, including miR-193b and miR-223, were recently described as deregulated in circulating peripheral blood mononuclear cells (PBMCs) from patients with psoriasis. Using flow cytometry and qRT-PCR, we found that miR-193b and miR-223 were expressed in Th17 cells. In conclusion, we demonstrate that LCM combined with NGS provides a robust approach to explore the global miRNA expression in the epidermal and dermal compartments of psoriatic skin. Furthermore, our results indicate that the altered local miRNA changes seen in the RD are reflected in the circulating immune cells, suggesting that miRNAs may contribute to the pathogenesis of psoriasis.

U2 - 10.1111/exd.12604

DO - 10.1111/exd.12604

M3 - Journal article

C2 - 25431026

VL - 24

SP - 187

EP - 193

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 3

ER -

ID: 134959520