In-gel digestion for mass spectrometric characterization of proteins and proteomes

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In-gel digestion for mass spectrometric characterization of proteins and proteomes. / Shevchenko, Andrej; Tomas, Henrik; Havlis, Jan; Olsen, Jesper Velgaard; Mann, Matthias.

In: Nature Protocols (Print Edition), Vol. 1, No. 6, 2006, p. 2856-60.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Shevchenko, A, Tomas, H, Havlis, J, Olsen, JV & Mann, M 2006, 'In-gel digestion for mass spectrometric characterization of proteins and proteomes', Nature Protocols (Print Edition), vol. 1, no. 6, pp. 2856-60. https://doi.org/10.1038/nprot.2006.468

APA

Shevchenko, A., Tomas, H., Havlis, J., Olsen, J. V., & Mann, M. (2006). In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols (Print Edition), 1(6), 2856-60. https://doi.org/10.1038/nprot.2006.468

Vancouver

Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols (Print Edition). 2006;1(6):2856-60. https://doi.org/10.1038/nprot.2006.468

Author

Shevchenko, Andrej ; Tomas, Henrik ; Havlis, Jan ; Olsen, Jesper Velgaard ; Mann, Matthias. / In-gel digestion for mass spectrometric characterization of proteins and proteomes. In: Nature Protocols (Print Edition). 2006 ; Vol. 1, No. 6. pp. 2856-60.

Bibtex

@article{d7a01dc7dcc24301893450b8555342aa,
title = "In-gel digestion for mass spectrometric characterization of proteins and proteomes",
abstract = "In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.",
author = "Andrej Shevchenko and Henrik Tomas and Jan Havlis and Olsen, {Jesper Velgaard} and Matthias Mann",
note = "Keywords: Electrophoresis; Mass Spectrometry; Proteins; Proteomics",
year = "2006",
doi = "10.1038/nprot.2006.468",
language = "English",
volume = "1",
pages = "2856--60",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "nature publishing group",
number = "6",

}

RIS

TY - JOUR

T1 - In-gel digestion for mass spectrometric characterization of proteins and proteomes

AU - Shevchenko, Andrej

AU - Tomas, Henrik

AU - Havlis, Jan

AU - Olsen, Jesper Velgaard

AU - Mann, Matthias

N1 - Keywords: Electrophoresis; Mass Spectrometry; Proteins; Proteomics

PY - 2006

Y1 - 2006

N2 - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

AB - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

U2 - 10.1038/nprot.2006.468

DO - 10.1038/nprot.2006.468

M3 - Journal article

C2 - 17406544

VL - 1

SP - 2856

EP - 2860

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 6

ER -

ID: 46460063