Identification of the MMS22L-TONSL complex that promotes homologous recombination
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Identification of the MMS22L-TONSL complex that promotes homologous recombination. / Duro, Eris; Lundin, Cecilia; Ask, Katrine; Sanchez-Pulido, Luis; MacArtney, Thomas J; Toth, Rachel; Ponting, Chris P; Groth, Anja; Helleday, Thomas; Rouse, John.
In: Molecular Cell, Vol. 40, No. 4, 24.11.2010, p. 632-44.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of the MMS22L-TONSL complex that promotes homologous recombination
AU - Duro, Eris
AU - Lundin, Cecilia
AU - Ask, Katrine
AU - Sanchez-Pulido, Luis
AU - MacArtney, Thomas J
AU - Toth, Rachel
AU - Ponting, Chris P
AU - Groth, Anja
AU - Helleday, Thomas
AU - Rouse, John
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010/11/24
Y1 - 2010/11/24
N2 - Budding yeast Mms22 is required for homologous recombination (HR)-mediated repair of stalled or broken DNA replication forks. Here we identify a human Mms22-like protein (MMS22L) and an MMS22L-interacting protein, NF¿BIL2/TONSL. Depletion of MMS22L or TONSL from human cells causes a high level of double-strand breaks (DSBs) during DNA replication. Both proteins accumulate at stressed replication forks, and depletion of MMS22L or TONSL from cells causes hypersensitivity to agents that cause S phase-associated DSBs, such as topoisomerase (TOP) inhibitors. In this light, MMS22L and TONSL are required for the HR-mediated repair of replication fork-associated DSBs. In cells depleted of either protein, DSBs induced by the TOP1 inhibitor camptothecin are resected normally, but the loading of the RAD51 recombinase is defective. Therefore, MMS22L and TONSL are required for the maintenance of genome stability when unscheduled DSBs occur in the vicinity of DNA replication forks.
AB - Budding yeast Mms22 is required for homologous recombination (HR)-mediated repair of stalled or broken DNA replication forks. Here we identify a human Mms22-like protein (MMS22L) and an MMS22L-interacting protein, NF¿BIL2/TONSL. Depletion of MMS22L or TONSL from human cells causes a high level of double-strand breaks (DSBs) during DNA replication. Both proteins accumulate at stressed replication forks, and depletion of MMS22L or TONSL from cells causes hypersensitivity to agents that cause S phase-associated DSBs, such as topoisomerase (TOP) inhibitors. In this light, MMS22L and TONSL are required for the HR-mediated repair of replication fork-associated DSBs. In cells depleted of either protein, DSBs induced by the TOP1 inhibitor camptothecin are resected normally, but the loading of the RAD51 recombinase is defective. Therefore, MMS22L and TONSL are required for the maintenance of genome stability when unscheduled DSBs occur in the vicinity of DNA replication forks.
KW - Amino Acid Sequence
KW - Cell Cycle Proteins
KW - Cell Line
KW - Cell Survival
KW - Computational Biology
KW - DNA Breaks, Double-Stranded
KW - DNA-Binding Proteins
KW - DNA-Directed DNA Polymerase
KW - Drug Resistance
KW - Humans
KW - Models, Biological
KW - Molecular Sequence Data
KW - Multienzyme Complexes
KW - Multiprotein Complexes
KW - NF-kappa B
KW - Nuclear Proteins
KW - Protein Binding
KW - Rad51 Recombinase
KW - Recombination, Genetic
KW - S Phase
U2 - 10.1016/j.molcel.2010.10.023
DO - 10.1016/j.molcel.2010.10.023
M3 - Journal article
C2 - 21055984
VL - 40
SP - 632
EP - 644
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 4
ER -
ID: 32309672