Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B. / Vázquez-Novelle, María D; Mailand, Niels; Ovejero, Sara; Bueno, Avelino; Sacristán, María P.

In: Journal of Biological Chemistry, Vol. 285, No. 52, 2010, p. 40544-53.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vázquez-Novelle, MD, Mailand, N, Ovejero, S, Bueno, A & Sacristán, MP 2010, 'Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B', Journal of Biological Chemistry, vol. 285, no. 52, pp. 40544-53. https://doi.org/10.1074/jbc.M110.133009

APA

Vázquez-Novelle, M. D., Mailand, N., Ovejero, S., Bueno, A., & Sacristán, M. P. (2010). Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B. Journal of Biological Chemistry, 285(52), 40544-53. https://doi.org/10.1074/jbc.M110.133009

Vancouver

Vázquez-Novelle MD, Mailand N, Ovejero S, Bueno A, Sacristán MP. Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B. Journal of Biological Chemistry. 2010;285(52):40544-53. https://doi.org/10.1074/jbc.M110.133009

Author

Vázquez-Novelle, María D ; Mailand, Niels ; Ovejero, Sara ; Bueno, Avelino ; Sacristán, María P. / Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 52. pp. 40544-53.

Bibtex

@article{5d2d215ebd7c42ffb3033ca2d75357c3,
title = "Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B",
abstract = "The Cdc14 family of serine-threonine phosphatases antagonizes CDK activity by reversing CDK-dependent phosphorylation events. It is well established that the yeast members of this family bring about the M/G1 transition. Budding yeast Cdc14 is essential for CDK inactivation at the end of mitosis and fission yeast Cdc14 homologue Flp1/Clp1 down-regulates Cdc25 to ensure the inactivation of mitotic CDK complexes to trigger cell division. However, the functions of human Cdc14 homologues remain poorly understood. Here we have tested the hypothesis that Cdc14A might regulate Cdc25 mitotic inducers in human cells. We found that increasing levels of Cdc14A delay entry into mitosis by inhibiting Cdk1-cyclin B1 activity. By contrast, lowering the levels of Cdc14A accelerates mitotic entry. Biochemical analyses revealed that Cdc14A acts through key Cdk1-cyclin B1 regulators. We observed that Cdc14A directly bound to and dephosphorylated Cdc25B, inhibiting its catalytic activity. Cdc14A also regulated the activity of Cdc25A at the G2/M transition. Our results indicate that Cdc14A phosphatase prevents premature activation of Cdk1 regulating Cdc25A and Cdc25B at the entry into mitosis.",
keywords = "CDC2 Protein Kinase, Cell Line, Enzyme Activation, G2 Phase, Humans, Mitosis, Phosphoric Monoester Hydrolases, Saccharomyces cerevisiae, cdc25 Phosphatases",
author = "V{\'a}zquez-Novelle, {Mar{\'i}a D} and Niels Mailand and Sara Ovejero and Avelino Bueno and Sacrist{\'a}n, {Mar{\'i}a P}",
year = "2010",
doi = "10.1074/jbc.M110.133009",
language = "English",
volume = "285",
pages = "40544--53",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "52",

}

RIS

TY - JOUR

T1 - Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B

AU - Vázquez-Novelle, María D

AU - Mailand, Niels

AU - Ovejero, Sara

AU - Bueno, Avelino

AU - Sacristán, María P

PY - 2010

Y1 - 2010

N2 - The Cdc14 family of serine-threonine phosphatases antagonizes CDK activity by reversing CDK-dependent phosphorylation events. It is well established that the yeast members of this family bring about the M/G1 transition. Budding yeast Cdc14 is essential for CDK inactivation at the end of mitosis and fission yeast Cdc14 homologue Flp1/Clp1 down-regulates Cdc25 to ensure the inactivation of mitotic CDK complexes to trigger cell division. However, the functions of human Cdc14 homologues remain poorly understood. Here we have tested the hypothesis that Cdc14A might regulate Cdc25 mitotic inducers in human cells. We found that increasing levels of Cdc14A delay entry into mitosis by inhibiting Cdk1-cyclin B1 activity. By contrast, lowering the levels of Cdc14A accelerates mitotic entry. Biochemical analyses revealed that Cdc14A acts through key Cdk1-cyclin B1 regulators. We observed that Cdc14A directly bound to and dephosphorylated Cdc25B, inhibiting its catalytic activity. Cdc14A also regulated the activity of Cdc25A at the G2/M transition. Our results indicate that Cdc14A phosphatase prevents premature activation of Cdk1 regulating Cdc25A and Cdc25B at the entry into mitosis.

AB - The Cdc14 family of serine-threonine phosphatases antagonizes CDK activity by reversing CDK-dependent phosphorylation events. It is well established that the yeast members of this family bring about the M/G1 transition. Budding yeast Cdc14 is essential for CDK inactivation at the end of mitosis and fission yeast Cdc14 homologue Flp1/Clp1 down-regulates Cdc25 to ensure the inactivation of mitotic CDK complexes to trigger cell division. However, the functions of human Cdc14 homologues remain poorly understood. Here we have tested the hypothesis that Cdc14A might regulate Cdc25 mitotic inducers in human cells. We found that increasing levels of Cdc14A delay entry into mitosis by inhibiting Cdk1-cyclin B1 activity. By contrast, lowering the levels of Cdc14A accelerates mitotic entry. Biochemical analyses revealed that Cdc14A acts through key Cdk1-cyclin B1 regulators. We observed that Cdc14A directly bound to and dephosphorylated Cdc25B, inhibiting its catalytic activity. Cdc14A also regulated the activity of Cdc25A at the G2/M transition. Our results indicate that Cdc14A phosphatase prevents premature activation of Cdk1 regulating Cdc25A and Cdc25B at the entry into mitosis.

KW - CDC2 Protein Kinase

KW - Cell Line

KW - Enzyme Activation

KW - G2 Phase

KW - Humans

KW - Mitosis

KW - Phosphoric Monoester Hydrolases

KW - Saccharomyces cerevisiae

KW - cdc25 Phosphatases

U2 - 10.1074/jbc.M110.133009

DO - 10.1074/jbc.M110.133009

M3 - Journal article

C2 - 20956543

VL - 285

SP - 40544

EP - 40553

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 52

ER -

ID: 40291211