Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo. / Martel, Britta C; Blom, Lars; Dyring-Andersen, Beatrice; Skov, Lone; Thestrup-Pedersen, Kristian; Skov, Søren; Skak, Kresten; Poulsen, Lars K.

In: Scandinavian Journal of Immunology, Vol. 82, No. 2, 22.05.2015, p. 125–134.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Martel, BC, Blom, L, Dyring-Andersen, B, Skov, L, Thestrup-Pedersen, K, Skov, S, Skak, K & Poulsen, LK 2015, 'Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo', Scandinavian Journal of Immunology, vol. 82, no. 2, pp. 125–134. https://doi.org/10.1111/sji.12316

APA

Martel, B. C., Blom, L., Dyring-Andersen, B., Skov, L., Thestrup-Pedersen, K., Skov, S., Skak, K., & Poulsen, L. K. (2015). Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo. Scandinavian Journal of Immunology, 82(2), 125–134. https://doi.org/10.1111/sji.12316

Vancouver

Martel BC, Blom L, Dyring-Andersen B, Skov L, Thestrup-Pedersen K, Skov S et al. Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo. Scandinavian Journal of Immunology. 2015 May 22;82(2):125–134. https://doi.org/10.1111/sji.12316

Author

Martel, Britta C ; Blom, Lars ; Dyring-Andersen, Beatrice ; Skov, Lone ; Thestrup-Pedersen, Kristian ; Skov, Søren ; Skak, Kresten ; Poulsen, Lars K. / Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo. In: Scandinavian Journal of Immunology. 2015 ; Vol. 82, No. 2. pp. 125–134.

Bibtex

@article{9878d2e1a07d434194240e034c64d3b8,
title = "Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo",
abstract = "In atopic dermatitis (AD), the inflammatory response between skin infiltrating T cells and keratinocytes is fundamental to the development of chronic lesional eczema. The aim of this study was to investigate whether skin-derived T cells from AD patients could induce an inflammatory response in mice through keratinocyte activation and consequently cause development of eczematous lesions. Punch biopsies of lesional skin from AD patients were used to establish skin-derived T cell cultures and which were transferred into NOD.Cg-Prkd(scid) Il2rg(tm1Sug) /JicTac (NOG) mice. We found that subcutaneous injection of the human AD skin-derived T cells resulted in migration of the human T cells from subcutis to the papillary dermis followed by development of erythema and edema in the mouse skin. Furthermore, the human T cells induced a transient proliferative response in the mouse keratinocytes shown as increased numbers of Ki-67(+) keratinocytes and increased epidermal thickness. Out of six established AD skin-derived T cell cultures, two were superior at inducing a skin reaction in the mice, and these cultures were found to contain >10% CCR10(+) T cells compared to <2% for the other cultures. In comparison, blood -derived in vitro differentiated Th2 cells only induced a weak response in a few of the mice. Thus, we conclude that human AD skin-derived T cells can induce a reaction in mouse skin through induction of a proliferative response in the mouse keratinocytes. This article is protected by copyright. All rights reserved.",
author = "Martel, {Britta C} and Lars Blom and Beatrice Dyring-Andersen and Lone Skov and Kristian Thestrup-Pedersen and S{\o}ren Skov and Kresten Skak and Poulsen, {Lars K}",
note = "This article is protected by copyright. All rights reserved.",
year = "2015",
month = may,
day = "22",
doi = "10.1111/sji.12316",
language = "English",
volume = "82",
pages = "125–134",
journal = "Scandinavian Journal of Immunology, Supplement",
issn = "0301-6323",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Human atopic dermatitis skin-derived T cells can induce a reaction in mouse keratinocytes in vivo

AU - Martel, Britta C

AU - Blom, Lars

AU - Dyring-Andersen, Beatrice

AU - Skov, Lone

AU - Thestrup-Pedersen, Kristian

AU - Skov, Søren

AU - Skak, Kresten

AU - Poulsen, Lars K

N1 - This article is protected by copyright. All rights reserved.

PY - 2015/5/22

Y1 - 2015/5/22

N2 - In atopic dermatitis (AD), the inflammatory response between skin infiltrating T cells and keratinocytes is fundamental to the development of chronic lesional eczema. The aim of this study was to investigate whether skin-derived T cells from AD patients could induce an inflammatory response in mice through keratinocyte activation and consequently cause development of eczematous lesions. Punch biopsies of lesional skin from AD patients were used to establish skin-derived T cell cultures and which were transferred into NOD.Cg-Prkd(scid) Il2rg(tm1Sug) /JicTac (NOG) mice. We found that subcutaneous injection of the human AD skin-derived T cells resulted in migration of the human T cells from subcutis to the papillary dermis followed by development of erythema and edema in the mouse skin. Furthermore, the human T cells induced a transient proliferative response in the mouse keratinocytes shown as increased numbers of Ki-67(+) keratinocytes and increased epidermal thickness. Out of six established AD skin-derived T cell cultures, two were superior at inducing a skin reaction in the mice, and these cultures were found to contain >10% CCR10(+) T cells compared to <2% for the other cultures. In comparison, blood -derived in vitro differentiated Th2 cells only induced a weak response in a few of the mice. Thus, we conclude that human AD skin-derived T cells can induce a reaction in mouse skin through induction of a proliferative response in the mouse keratinocytes. This article is protected by copyright. All rights reserved.

AB - In atopic dermatitis (AD), the inflammatory response between skin infiltrating T cells and keratinocytes is fundamental to the development of chronic lesional eczema. The aim of this study was to investigate whether skin-derived T cells from AD patients could induce an inflammatory response in mice through keratinocyte activation and consequently cause development of eczematous lesions. Punch biopsies of lesional skin from AD patients were used to establish skin-derived T cell cultures and which were transferred into NOD.Cg-Prkd(scid) Il2rg(tm1Sug) /JicTac (NOG) mice. We found that subcutaneous injection of the human AD skin-derived T cells resulted in migration of the human T cells from subcutis to the papillary dermis followed by development of erythema and edema in the mouse skin. Furthermore, the human T cells induced a transient proliferative response in the mouse keratinocytes shown as increased numbers of Ki-67(+) keratinocytes and increased epidermal thickness. Out of six established AD skin-derived T cell cultures, two were superior at inducing a skin reaction in the mice, and these cultures were found to contain >10% CCR10(+) T cells compared to <2% for the other cultures. In comparison, blood -derived in vitro differentiated Th2 cells only induced a weak response in a few of the mice. Thus, we conclude that human AD skin-derived T cells can induce a reaction in mouse skin through induction of a proliferative response in the mouse keratinocytes. This article is protected by copyright. All rights reserved.

U2 - 10.1111/sji.12316

DO - 10.1111/sji.12316

M3 - Journal article

C2 - 25998164

VL - 82

SP - 125

EP - 134

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

SN - 0301-6323

IS - 2

ER -

ID: 140439126