Higher-order modular regulation of the human proteome

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Higher-order modular regulation of the human proteome. / Kustatscher, Georg; Hödl, Martina; Rullmann, Edward; Grabowski, Piotr; Fiagbedzi, Emmanuel; Groth, Anja; Rappsilber, Juri.

In: Molecular Systems Biology, Vol. 19, No. 5, e9503, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kustatscher, G, Hödl, M, Rullmann, E, Grabowski, P, Fiagbedzi, E, Groth, A & Rappsilber, J 2023, 'Higher-order modular regulation of the human proteome', Molecular Systems Biology, vol. 19, no. 5, e9503. https://doi.org/10.15252/msb.20209503

APA

Kustatscher, G., Hödl, M., Rullmann, E., Grabowski, P., Fiagbedzi, E., Groth, A., & Rappsilber, J. (2023). Higher-order modular regulation of the human proteome. Molecular Systems Biology, 19(5), [e9503]. https://doi.org/10.15252/msb.20209503

Vancouver

Kustatscher G, Hödl M, Rullmann E, Grabowski P, Fiagbedzi E, Groth A et al. Higher-order modular regulation of the human proteome. Molecular Systems Biology. 2023;19(5). e9503. https://doi.org/10.15252/msb.20209503

Author

Kustatscher, Georg ; Hödl, Martina ; Rullmann, Edward ; Grabowski, Piotr ; Fiagbedzi, Emmanuel ; Groth, Anja ; Rappsilber, Juri. / Higher-order modular regulation of the human proteome. In: Molecular Systems Biology. 2023 ; Vol. 19, No. 5.

Bibtex

@article{4099585536e840dcb2d7acb57f788b49,
title = "Higher-order modular regulation of the human proteome",
abstract = "Operons are transcriptional modules that allow bacteria to adapt to environmental changes by coordinately expressing the relevant set of genes. In humans, biological pathways and their regulation are more complex. If and how human cells coordinate the expression of entire biological processes is unclear. Here, we capture 31 higher-order co-regulation modules, which we term progulons, by help of supervised machine-learning on proteomics data. Progulons consist of dozens to hundreds of proteins that together mediate core cellular functions. They are not restricted to physical interactions or co-localisation. Progulon abundance changes are primarily controlled at the level of protein synthesis and degradation. Implemented as a web app at www.proteomehd.net/progulonFinder, our approach enables the targeted search for progulons of specific cellular processes. We use it to identify a DNA replication progulon and reveal multiple new replication factors, validated by extensive phenotyping of siRNA-induced knockdowns. Progulons provide a new entry point into the molecular understanding of biological processes.",
author = "Georg Kustatscher and Martina H{\"o}dl and Edward Rullmann and Piotr Grabowski and Emmanuel Fiagbedzi and Anja Groth and Juri Rappsilber",
note = "{\textcopyright} 2023 The Authors. Published under the terms of the CC BY 4.0 license.",
year = "2023",
doi = "10.15252/msb.20209503",
language = "English",
volume = "19",
journal = "Molecular Systems Biology",
issn = "1744-4292",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Higher-order modular regulation of the human proteome

AU - Kustatscher, Georg

AU - Hödl, Martina

AU - Rullmann, Edward

AU - Grabowski, Piotr

AU - Fiagbedzi, Emmanuel

AU - Groth, Anja

AU - Rappsilber, Juri

N1 - © 2023 The Authors. Published under the terms of the CC BY 4.0 license.

PY - 2023

Y1 - 2023

N2 - Operons are transcriptional modules that allow bacteria to adapt to environmental changes by coordinately expressing the relevant set of genes. In humans, biological pathways and their regulation are more complex. If and how human cells coordinate the expression of entire biological processes is unclear. Here, we capture 31 higher-order co-regulation modules, which we term progulons, by help of supervised machine-learning on proteomics data. Progulons consist of dozens to hundreds of proteins that together mediate core cellular functions. They are not restricted to physical interactions or co-localisation. Progulon abundance changes are primarily controlled at the level of protein synthesis and degradation. Implemented as a web app at www.proteomehd.net/progulonFinder, our approach enables the targeted search for progulons of specific cellular processes. We use it to identify a DNA replication progulon and reveal multiple new replication factors, validated by extensive phenotyping of siRNA-induced knockdowns. Progulons provide a new entry point into the molecular understanding of biological processes.

AB - Operons are transcriptional modules that allow bacteria to adapt to environmental changes by coordinately expressing the relevant set of genes. In humans, biological pathways and their regulation are more complex. If and how human cells coordinate the expression of entire biological processes is unclear. Here, we capture 31 higher-order co-regulation modules, which we term progulons, by help of supervised machine-learning on proteomics data. Progulons consist of dozens to hundreds of proteins that together mediate core cellular functions. They are not restricted to physical interactions or co-localisation. Progulon abundance changes are primarily controlled at the level of protein synthesis and degradation. Implemented as a web app at www.proteomehd.net/progulonFinder, our approach enables the targeted search for progulons of specific cellular processes. We use it to identify a DNA replication progulon and reveal multiple new replication factors, validated by extensive phenotyping of siRNA-induced knockdowns. Progulons provide a new entry point into the molecular understanding of biological processes.

U2 - 10.15252/msb.20209503

DO - 10.15252/msb.20209503

M3 - Journal article

C2 - 36891684

VL - 19

JO - Molecular Systems Biology

JF - Molecular Systems Biology

SN - 1744-4292

IS - 5

M1 - e9503

ER -

ID: 340364901