Generation and diversification of recombinant monoclonal antibodies

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Generation and diversification of recombinant monoclonal antibodies. / DeLuca, Keith F; Mick, Jeanne E; Ide, Amy Hodges; Lima, Wanessa C.; Sherman, Lori; Schaller, Kristin L; Anderson, Steven M; Zhao, Ning; Stasevich, Timothy J; Varma, Dileep; Nilsson, Jakob; DeLuca, Jennifer G.

In: eLife, Vol. 10, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

DeLuca, KF, Mick, JE, Ide, AH, Lima, WC, Sherman, L, Schaller, KL, Anderson, SM, Zhao, N, Stasevich, TJ, Varma, D, Nilsson, J & DeLuca, JG 2021, 'Generation and diversification of recombinant monoclonal antibodies', eLife, vol. 10. https://doi.org/10.7554/eLife.72093

APA

DeLuca, K. F., Mick, J. E., Ide, A. H., Lima, W. C., Sherman, L., Schaller, K. L., Anderson, S. M., Zhao, N., Stasevich, T. J., Varma, D., Nilsson, J., & DeLuca, J. G. (2021). Generation and diversification of recombinant monoclonal antibodies. eLife, 10. https://doi.org/10.7554/eLife.72093

Vancouver

DeLuca KF, Mick JE, Ide AH, Lima WC, Sherman L, Schaller KL et al. Generation and diversification of recombinant monoclonal antibodies. eLife. 2021;10. https://doi.org/10.7554/eLife.72093

Author

DeLuca, Keith F ; Mick, Jeanne E ; Ide, Amy Hodges ; Lima, Wanessa C. ; Sherman, Lori ; Schaller, Kristin L ; Anderson, Steven M ; Zhao, Ning ; Stasevich, Timothy J ; Varma, Dileep ; Nilsson, Jakob ; DeLuca, Jennifer G. / Generation and diversification of recombinant monoclonal antibodies. In: eLife. 2021 ; Vol. 10.

Bibtex

@article{6ddeea9eb2da46519ad9045b32efe0cb,
title = "Generation and diversification of recombinant monoclonal antibodies",
abstract = "Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standardization leading to problems with reproducibility, high costs of antibodies purchased from commercial sources, and ethical concerns regarding the large number of animals used to generate antibodies. To address these issues, we have developed practical methodologies and tools for generating low-cost, high-yield preparations of recombinant monoclonal antibodies and antibody fragments directed to protein epitopes from primary sequences. We describe these methods here, as well as approaches to diversify monoclonal antibodies, including customization of antibody species specificity, generation of genetically encoded small antibody fragments, and conversion of single chain antibody fragments (e.g. scFv) into full-length, bivalent antibodies. This study focuses on antibodies directed to epitopes important for mitosis and kinetochore function; however, the methods and reagents described here are applicable to antibodies and antibody fragments for use in any field.",
author = "DeLuca, {Keith F} and Mick, {Jeanne E} and Ide, {Amy Hodges} and Lima, {Wanessa C.} and Lori Sherman and Schaller, {Kristin L} and Anderson, {Steven M} and Ning Zhao and Stasevich, {Timothy J} and Dileep Varma and Jakob Nilsson and DeLuca, {Jennifer G}",
note = "{\textcopyright} 2021, DeLuca et al.",
year = "2021",
doi = "10.7554/eLife.72093",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Generation and diversification of recombinant monoclonal antibodies

AU - DeLuca, Keith F

AU - Mick, Jeanne E

AU - Ide, Amy Hodges

AU - Lima, Wanessa C.

AU - Sherman, Lori

AU - Schaller, Kristin L

AU - Anderson, Steven M

AU - Zhao, Ning

AU - Stasevich, Timothy J

AU - Varma, Dileep

AU - Nilsson, Jakob

AU - DeLuca, Jennifer G

N1 - © 2021, DeLuca et al.

PY - 2021

Y1 - 2021

N2 - Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standardization leading to problems with reproducibility, high costs of antibodies purchased from commercial sources, and ethical concerns regarding the large number of animals used to generate antibodies. To address these issues, we have developed practical methodologies and tools for generating low-cost, high-yield preparations of recombinant monoclonal antibodies and antibody fragments directed to protein epitopes from primary sequences. We describe these methods here, as well as approaches to diversify monoclonal antibodies, including customization of antibody species specificity, generation of genetically encoded small antibody fragments, and conversion of single chain antibody fragments (e.g. scFv) into full-length, bivalent antibodies. This study focuses on antibodies directed to epitopes important for mitosis and kinetochore function; however, the methods and reagents described here are applicable to antibodies and antibody fragments for use in any field.

AB - Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standardization leading to problems with reproducibility, high costs of antibodies purchased from commercial sources, and ethical concerns regarding the large number of animals used to generate antibodies. To address these issues, we have developed practical methodologies and tools for generating low-cost, high-yield preparations of recombinant monoclonal antibodies and antibody fragments directed to protein epitopes from primary sequences. We describe these methods here, as well as approaches to diversify monoclonal antibodies, including customization of antibody species specificity, generation of genetically encoded small antibody fragments, and conversion of single chain antibody fragments (e.g. scFv) into full-length, bivalent antibodies. This study focuses on antibodies directed to epitopes important for mitosis and kinetochore function; however, the methods and reagents described here are applicable to antibodies and antibody fragments for use in any field.

U2 - 10.7554/eLife.72093

DO - 10.7554/eLife.72093

M3 - Journal article

C2 - 34970967

VL - 10

JO - eLife

JF - eLife

SN - 2050-084X

ER -

ID: 289964532