Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling

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Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling. / Zhang, Yuqing; Young, Rose; Garvanska, Dimitriya H.; Song, Chunlin; Zhai, Yujing; Wang, Ying; Jiang, Hongfei; Fang, Jing; Nilsson, Jakob; Alfieri, Claudio; Zhang, Gang.

In: Communications Biology , Vol. 7, 164, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, Y, Young, R, Garvanska, DH, Song, C, Zhai, Y, Wang, Y, Jiang, H, Fang, J, Nilsson, J, Alfieri, C & Zhang, G 2024, 'Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling', Communications Biology , vol. 7, 164. https://doi.org/10.1038/s42003-024-05859-6

APA

Zhang, Y., Young, R., Garvanska, D. H., Song, C., Zhai, Y., Wang, Y., Jiang, H., Fang, J., Nilsson, J., Alfieri, C., & Zhang, G. (2024). Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling. Communications Biology , 7, [164]. https://doi.org/10.1038/s42003-024-05859-6

Vancouver

Zhang Y, Young R, Garvanska DH, Song C, Zhai Y, Wang Y et al. Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling. Communications Biology . 2024;7. 164. https://doi.org/10.1038/s42003-024-05859-6

Author

Zhang, Yuqing ; Young, Rose ; Garvanska, Dimitriya H. ; Song, Chunlin ; Zhai, Yujing ; Wang, Ying ; Jiang, Hongfei ; Fang, Jing ; Nilsson, Jakob ; Alfieri, Claudio ; Zhang, Gang. / Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling. In: Communications Biology . 2024 ; Vol. 7.

Bibtex

@article{77666f64d5bf47f8ae74a1b5db290eec,
title = "Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling",
abstract = "Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through activating SAC. Cdc20 requires multiple interactions with APC/C and MCC subunits to elicit these functions. Functionally assessing these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNA interference (RNAi). Here we generated Cdc20 RNAi-sensitive cell lines which display a penetrant metaphase arrest by a single RNAi treatment. In this null background, we accurately measured the contribution of each known motif of Cdc20 on APC/C and SAC activation. The CRY box, a previously identified degron, was found critical for SAC by promoting MCC formation and its interaction with APC/C. These data reveal additional regulation within the SAC and establish a novel method to interrogate Cdc20.",
author = "Yuqing Zhang and Rose Young and Garvanska, {Dimitriya H.} and Chunlin Song and Yujing Zhai and Ying Wang and Hongfei Jiang and Jing Fang and Jakob Nilsson and Claudio Alfieri and Gang Zhang",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",
year = "2024",
doi = "10.1038/s42003-024-05859-6",
language = "English",
volume = "7",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling

AU - Zhang, Yuqing

AU - Young, Rose

AU - Garvanska, Dimitriya H.

AU - Song, Chunlin

AU - Zhai, Yujing

AU - Wang, Ying

AU - Jiang, Hongfei

AU - Fang, Jing

AU - Nilsson, Jakob

AU - Alfieri, Claudio

AU - Zhang, Gang

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024

Y1 - 2024

N2 - Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through activating SAC. Cdc20 requires multiple interactions with APC/C and MCC subunits to elicit these functions. Functionally assessing these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNA interference (RNAi). Here we generated Cdc20 RNAi-sensitive cell lines which display a penetrant metaphase arrest by a single RNAi treatment. In this null background, we accurately measured the contribution of each known motif of Cdc20 on APC/C and SAC activation. The CRY box, a previously identified degron, was found critical for SAC by promoting MCC formation and its interaction with APC/C. These data reveal additional regulation within the SAC and establish a novel method to interrogate Cdc20.

AB - Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through activating SAC. Cdc20 requires multiple interactions with APC/C and MCC subunits to elicit these functions. Functionally assessing these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNA interference (RNAi). Here we generated Cdc20 RNAi-sensitive cell lines which display a penetrant metaphase arrest by a single RNAi treatment. In this null background, we accurately measured the contribution of each known motif of Cdc20 on APC/C and SAC activation. The CRY box, a previously identified degron, was found critical for SAC by promoting MCC formation and its interaction with APC/C. These data reveal additional regulation within the SAC and establish a novel method to interrogate Cdc20.

U2 - 10.1038/s42003-024-05859-6

DO - 10.1038/s42003-024-05859-6

M3 - Journal article

C2 - 38337031

AN - SCOPUS:85184786340

VL - 7

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

M1 - 164

ER -

ID: 383398084