Engineering dynamic pathway regulation using stress-response promoters

Research output: Contribution to journal › Journal article › Research › peer-review

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Engineering dynamic pathway regulation using stress-response promoters. / Dahl, Robert H; Zhang, Fuzhong; Alonso-Gutierrez, Jorge; Baidoo, Edward; Batth, Tanveer S; Redding-Johanson, Alyssa M; Petzold, Christopher J; Mukhopadhyay, Aindrila; Lee, Taek Soon; Adams, Paul D; Keasling, Jay D.

In: Nature Biotechnology, 20.10.2013.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dahl, RH, Zhang, F, Alonso-Gutierrez, J, Baidoo, E, Batth, TS, Redding-Johanson, AM, Petzold, CJ, Mukhopadhyay, A, Lee, TS, Adams, PD & Keasling, JD 2013, 'Engineering dynamic pathway regulation using stress-response promoters', Nature Biotechnology. https://doi.org/10.1038/nbt.2689

APA

Dahl, R. H., Zhang, F., Alonso-Gutierrez, J., Baidoo, E., Batth, T. S., Redding-Johanson, A. M., Petzold, C. J., Mukhopadhyay, A., Lee, T. S., Adams, P. D., & Keasling, J. D. (2013). Engineering dynamic pathway regulation using stress-response promoters. Nature Biotechnology. https://doi.org/10.1038/nbt.2689

Vancouver

Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, Redding-Johanson AM et al. Engineering dynamic pathway regulation using stress-response promoters. Nature Biotechnology. 2013 Oct 20. https://doi.org/10.1038/nbt.2689

Author

Dahl, Robert H ; Zhang, Fuzhong ; Alonso-Gutierrez, Jorge ; Baidoo, Edward ; Batth, Tanveer S ; Redding-Johanson, Alyssa M ; Petzold, Christopher J ; Mukhopadhyay, Aindrila ; Lee, Taek Soon ; Adams, Paul D ; Keasling, Jay D. / Engineering dynamic pathway regulation using stress-response promoters. In: Nature Biotechnology. 2013.

Bibtex

@article{b8294aa2265149a3bf90b628764ccec5,
title = "Engineering dynamic pathway regulation using stress-response promoters",
abstract = "Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-genome transcript arrays to identify promoters that respond to the accumulation of toxic intermediates, and then used these promoters to control accumulation of the intermediate and improve the final titers of a desired product. We apply this approach to regulate farnesyl pyrophosphate (FPP) production in the isoprenoid biosynthetic pathway in Escherichia coli. This strategy improved production of amorphadiene, the final product, by twofold over that from inducible or constitutive promoters, eliminated the need for expensive inducers, reduced acetate accumulation and improved growth. We extended this approach to another toxic intermediate to demonstrate the broad utility of identifying novel sensor-regulator systems for dynamic regulation.",
author = "Dahl, {Robert H} and Fuzhong Zhang and Jorge Alonso-Gutierrez and Edward Baidoo and Batth, {Tanveer S} and Redding-Johanson, {Alyssa M} and Petzold, {Christopher J} and Aindrila Mukhopadhyay and Lee, {Taek Soon} and Adams, {Paul D} and Keasling, {Jay D}",
year = "2013",
month = oct,
day = "20",
doi = "10.1038/nbt.2689",
language = "English",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Engineering dynamic pathway regulation using stress-response promoters

AU - Dahl, Robert H

AU - Zhang, Fuzhong

AU - Alonso-Gutierrez, Jorge

AU - Baidoo, Edward

AU - Batth, Tanveer S

AU - Redding-Johanson, Alyssa M

AU - Petzold, Christopher J

AU - Mukhopadhyay, Aindrila

AU - Lee, Taek Soon

AU - Adams, Paul D

AU - Keasling, Jay D

PY - 2013/10/20

Y1 - 2013/10/20

N2 - Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-genome transcript arrays to identify promoters that respond to the accumulation of toxic intermediates, and then used these promoters to control accumulation of the intermediate and improve the final titers of a desired product. We apply this approach to regulate farnesyl pyrophosphate (FPP) production in the isoprenoid biosynthetic pathway in Escherichia coli. This strategy improved production of amorphadiene, the final product, by twofold over that from inducible or constitutive promoters, eliminated the need for expensive inducers, reduced acetate accumulation and improved growth. We extended this approach to another toxic intermediate to demonstrate the broad utility of identifying novel sensor-regulator systems for dynamic regulation.

AB - Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-genome transcript arrays to identify promoters that respond to the accumulation of toxic intermediates, and then used these promoters to control accumulation of the intermediate and improve the final titers of a desired product. We apply this approach to regulate farnesyl pyrophosphate (FPP) production in the isoprenoid biosynthetic pathway in Escherichia coli. This strategy improved production of amorphadiene, the final product, by twofold over that from inducible or constitutive promoters, eliminated the need for expensive inducers, reduced acetate accumulation and improved growth. We extended this approach to another toxic intermediate to demonstrate the broad utility of identifying novel sensor-regulator systems for dynamic regulation.

U2 - 10.1038/nbt.2689

DO - 10.1038/nbt.2689

M3 - Journal article

C2 - 24142050

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

ER -

ID: 68163506