Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3.
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Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3. / Lindroth, Anders M; Shultis, David; Jasencakova, Zusana; Fuchs, Jörg; Johnson, Lianna; Schubert, Daniel; Patnaik, Debasis; Pradhan, Sriharsa; Goodrich, Justin; Schubert, Ingo; Jenuwein, Thomas; Khorasanizadeh, Sepideh; Jacobsen, Steven E.
In: EMBO Journal, Vol. 23, No. 21, 2004, p. 4286-96.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3.
AU - Lindroth, Anders M
AU - Shultis, David
AU - Jasencakova, Zusana
AU - Fuchs, Jörg
AU - Johnson, Lianna
AU - Schubert, Daniel
AU - Patnaik, Debasis
AU - Pradhan, Sriharsa
AU - Goodrich, Justin
AU - Schubert, Ingo
AU - Jenuwein, Thomas
AU - Khorasanizadeh, Sepideh
AU - Jacobsen, Steven E
N1 - Keywords: Amino Acid Sequence; Animals; Arabidopsis; Arabidopsis Proteins; Chromosomal Proteins, Non-Histone; Epigenesis, Genetic; Gene Expression Regulation, Plant; Gene Silencing; Histone-Lysine N-Methyltransferase; Histones; Humans; Lysine; Methylation; Methyltransferases; Models, Genetic; Molecular Sequence Data; Protein Binding; Sequence Alignment; Site-Specific DNA Methyltransferase (Cytosine-Specific)
PY - 2004
Y1 - 2004
N2 - Both DNA methylation and post-translational histone modifications contribute to gene silencing, but the mechanistic relationship between these epigenetic marks is unclear. Mutations in two Arabidopsis genes, the KRYPTONITE (KYP) histone H3 lysine 9 (H3K9) methyltransferase and the CHROMOMETHYLASE3 (CMT3) DNA methyltransferase, cause a reduction of CNG DNA methylation, suggesting that H3K9 methylation controls CNG DNA methylation. Here we show that the chromodomain of CMT3 can directly interact with the N-terminal tail of histone H3, but only when it is simultaneously methylated at both the H3K9 and H3K27 positions. Furthermore, using chromatin immunoprecipitation analysis and immunohistolocalization experiments, we found that H3K27 methylation colocalizes with H3K9 methylation at CMT3-controlled loci. The H3K27 methylation present at heterochromatin was not affected by mutations in KYP or in several Arabidopsis PcG related genes including the Enhancer of Zeste homologs, suggesting that a novel pathway controls heterochromatic H3K27 methylation. Our results suggest a model in which H3K9 methylation by KYP, and H3K27 methylation by an unknown enzyme provide a combinatorial histone code for the recruitment of CMT3 to silent loci.
AB - Both DNA methylation and post-translational histone modifications contribute to gene silencing, but the mechanistic relationship between these epigenetic marks is unclear. Mutations in two Arabidopsis genes, the KRYPTONITE (KYP) histone H3 lysine 9 (H3K9) methyltransferase and the CHROMOMETHYLASE3 (CMT3) DNA methyltransferase, cause a reduction of CNG DNA methylation, suggesting that H3K9 methylation controls CNG DNA methylation. Here we show that the chromodomain of CMT3 can directly interact with the N-terminal tail of histone H3, but only when it is simultaneously methylated at both the H3K9 and H3K27 positions. Furthermore, using chromatin immunoprecipitation analysis and immunohistolocalization experiments, we found that H3K27 methylation colocalizes with H3K9 methylation at CMT3-controlled loci. The H3K27 methylation present at heterochromatin was not affected by mutations in KYP or in several Arabidopsis PcG related genes including the Enhancer of Zeste homologs, suggesting that a novel pathway controls heterochromatic H3K27 methylation. Our results suggest a model in which H3K9 methylation by KYP, and H3K27 methylation by an unknown enzyme provide a combinatorial histone code for the recruitment of CMT3 to silent loci.
U2 - 10.1038/sj.emboj.7600430
DO - 10.1038/sj.emboj.7600430
M3 - Journal article
C2 - 15457214
VL - 23
SP - 4286
EP - 4296
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 21
ER -
ID: 5014103