Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis. / Martel, Britta Cathrina; Dyring-Andersen, Beatrice; Skov, Lone; Thestrup-Pedersen, Kristian; Skov, Søren; Skak, Kresten; Poulsen, Lars K.

In: Inflammation Research, Vol. 65, No. 4, 04.2016, p. 265-72.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Martel, BC, Dyring-Andersen, B, Skov, L, Thestrup-Pedersen, K, Skov, S, Skak, K & Poulsen, LK 2016, 'Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis', Inflammation Research, vol. 65, no. 4, pp. 265-72. https://doi.org/10.1007/s00011-015-0912-z

APA

Martel, B. C., Dyring-Andersen, B., Skov, L., Thestrup-Pedersen, K., Skov, S., Skak, K., & Poulsen, L. K. (2016). Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis. Inflammation Research, 65(4), 265-72. https://doi.org/10.1007/s00011-015-0912-z

Vancouver

Martel BC, Dyring-Andersen B, Skov L, Thestrup-Pedersen K, Skov S, Skak K et al. Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis. Inflammation Research. 2016 Apr;65(4):265-72. https://doi.org/10.1007/s00011-015-0912-z

Author

Martel, Britta Cathrina ; Dyring-Andersen, Beatrice ; Skov, Lone ; Thestrup-Pedersen, Kristian ; Skov, Søren ; Skak, Kresten ; Poulsen, Lars K. / Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis. In: Inflammation Research. 2016 ; Vol. 65, No. 4. pp. 265-72.

Bibtex

@article{0fada10d50c147d780f77f165b055571,
title = "Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis",
abstract = "OBJECTIVES: To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.MATERIAL: Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).METHODS: Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.RESULTS: A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.CONCLUSION: The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "Martel, {Britta Cathrina} and Beatrice Dyring-Andersen and Lone Skov and Kristian Thestrup-Pedersen and S{\o}ren Skov and Kresten Skak and Poulsen, {Lars K}",
year = "2016",
month = apr,
doi = "10.1007/s00011-015-0912-z",
language = "English",
volume = "65",
pages = "265--72",
journal = "Inflammation Research",
issn = "1023-3830",
publisher = "Springer Basel AG",
number = "4",

}

RIS

TY - JOUR

T1 - Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis

AU - Martel, Britta Cathrina

AU - Dyring-Andersen, Beatrice

AU - Skov, Lone

AU - Thestrup-Pedersen, Kristian

AU - Skov, Søren

AU - Skak, Kresten

AU - Poulsen, Lars K

PY - 2016/4

Y1 - 2016/4

N2 - OBJECTIVES: To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.MATERIAL: Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).METHODS: Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.RESULTS: A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.CONCLUSION: The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.

AB - OBJECTIVES: To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.MATERIAL: Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).METHODS: Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.RESULTS: A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.CONCLUSION: The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00011-015-0912-z

DO - 10.1007/s00011-015-0912-z

M3 - Journal article

C2 - 26722669

VL - 65

SP - 265

EP - 272

JO - Inflammation Research

JF - Inflammation Research

SN - 1023-3830

IS - 4

ER -

ID: 165663828