Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. / Klein, Ditte Kjærsgaard; Hoffmann, Saskia; Ahlskog, Johanna K; O'Hanlon, Karen; Quaas, Marianne; Larsen, Brian D; Rolland, Baptiste; Rösner, Heike I; Walter, David; Kousholt, Arne Nedergaard; Menzel, Tobias; Lees, Michael; Johansen, Jens Vilstrup; Rappsilber, Juri; Engeland, Kurt; Sørensen, Claus Storgaard.

In: Nature Communications, Vol. 6, 2015, p. 5800.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klein, DK, Hoffmann, S, Ahlskog, JK, O'Hanlon, K, Quaas, M, Larsen, BD, Rolland, B, Rösner, HI, Walter, D, Kousholt, AN, Menzel, T, Lees, M, Johansen, JV, Rappsilber, J, Engeland, K & Sørensen, CS 2015, 'Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control', Nature Communications, vol. 6, pp. 5800. https://doi.org/10.1038/ncomms6800

APA

Klein, D. K., Hoffmann, S., Ahlskog, J. K., O'Hanlon, K., Quaas, M., Larsen, B. D., Rolland, B., Rösner, H. I., Walter, D., Kousholt, A. N., Menzel, T., Lees, M., Johansen, J. V., Rappsilber, J., Engeland, K., & Sørensen, C. S. (2015). Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. Nature Communications, 6, 5800. https://doi.org/10.1038/ncomms6800

Vancouver

Klein DK, Hoffmann S, Ahlskog JK, O'Hanlon K, Quaas M, Larsen BD et al. Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. Nature Communications. 2015;6:5800. https://doi.org/10.1038/ncomms6800

Author

Klein, Ditte Kjærsgaard ; Hoffmann, Saskia ; Ahlskog, Johanna K ; O'Hanlon, Karen ; Quaas, Marianne ; Larsen, Brian D ; Rolland, Baptiste ; Rösner, Heike I ; Walter, David ; Kousholt, Arne Nedergaard ; Menzel, Tobias ; Lees, Michael ; Johansen, Jens Vilstrup ; Rappsilber, Juri ; Engeland, Kurt ; Sørensen, Claus Storgaard. / Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. In: Nature Communications. 2015 ; Vol. 6. pp. 5800.

Bibtex

@article{49ad0ad1738e4f20a445b2a0d04b3dff,
title = "Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control",
abstract = "Cells respond to DNA damage by activating cell cycle checkpoints to delay proliferation and facilitate DNA repair. Here, to uncover new checkpoint regulators, we perform RNA interference screening targeting genes involved in ubiquitylation processes. We show that the F-box protein cyclin F plays an important role in checkpoint control following ionizing radiation. Cyclin F-depleted cells initiate checkpoint signalling after ionizing radiation, but fail to maintain G2 phase arrest and progress into mitosis prematurely. Importantly, cyclin F suppresses the B-Myb-driven transcriptional programme that promotes accumulation of crucial mitosis-promoting proteins. Cyclin F interacts with B-Myb via the cyclin box domain. This interaction is important to suppress cyclin A-mediated phosphorylation of B-Myb, a key step in B-Myb activation. In summary, we uncover a regulatory mechanism linking the F-box protein cyclin F with suppression of the B-Myb/cyclin A pathway to ensure a DNA damage-induced checkpoint response in G2.",
author = "Klein, {Ditte Kj{\ae}rsgaard} and Saskia Hoffmann and Ahlskog, {Johanna K} and Karen O'Hanlon and Marianne Quaas and Larsen, {Brian D} and Baptiste Rolland and R{\"o}sner, {Heike I} and David Walter and Kousholt, {Arne Nedergaard} and Tobias Menzel and Michael Lees and Johansen, {Jens Vilstrup} and Juri Rappsilber and Kurt Engeland and S{\o}rensen, {Claus Storgaard}",
year = "2015",
doi = "10.1038/ncomms6800",
language = "English",
volume = "6",
pages = "5800",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control

AU - Klein, Ditte Kjærsgaard

AU - Hoffmann, Saskia

AU - Ahlskog, Johanna K

AU - O'Hanlon, Karen

AU - Quaas, Marianne

AU - Larsen, Brian D

AU - Rolland, Baptiste

AU - Rösner, Heike I

AU - Walter, David

AU - Kousholt, Arne Nedergaard

AU - Menzel, Tobias

AU - Lees, Michael

AU - Johansen, Jens Vilstrup

AU - Rappsilber, Juri

AU - Engeland, Kurt

AU - Sørensen, Claus Storgaard

PY - 2015

Y1 - 2015

N2 - Cells respond to DNA damage by activating cell cycle checkpoints to delay proliferation and facilitate DNA repair. Here, to uncover new checkpoint regulators, we perform RNA interference screening targeting genes involved in ubiquitylation processes. We show that the F-box protein cyclin F plays an important role in checkpoint control following ionizing radiation. Cyclin F-depleted cells initiate checkpoint signalling after ionizing radiation, but fail to maintain G2 phase arrest and progress into mitosis prematurely. Importantly, cyclin F suppresses the B-Myb-driven transcriptional programme that promotes accumulation of crucial mitosis-promoting proteins. Cyclin F interacts with B-Myb via the cyclin box domain. This interaction is important to suppress cyclin A-mediated phosphorylation of B-Myb, a key step in B-Myb activation. In summary, we uncover a regulatory mechanism linking the F-box protein cyclin F with suppression of the B-Myb/cyclin A pathway to ensure a DNA damage-induced checkpoint response in G2.

AB - Cells respond to DNA damage by activating cell cycle checkpoints to delay proliferation and facilitate DNA repair. Here, to uncover new checkpoint regulators, we perform RNA interference screening targeting genes involved in ubiquitylation processes. We show that the F-box protein cyclin F plays an important role in checkpoint control following ionizing radiation. Cyclin F-depleted cells initiate checkpoint signalling after ionizing radiation, but fail to maintain G2 phase arrest and progress into mitosis prematurely. Importantly, cyclin F suppresses the B-Myb-driven transcriptional programme that promotes accumulation of crucial mitosis-promoting proteins. Cyclin F interacts with B-Myb via the cyclin box domain. This interaction is important to suppress cyclin A-mediated phosphorylation of B-Myb, a key step in B-Myb activation. In summary, we uncover a regulatory mechanism linking the F-box protein cyclin F with suppression of the B-Myb/cyclin A pathway to ensure a DNA damage-induced checkpoint response in G2.

U2 - 10.1038/ncomms6800

DO - 10.1038/ncomms6800

M3 - Journal article

C2 - 25557911

VL - 6

SP - 5800

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

ER -

ID: 138140926