Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo

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Standard

Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo. / Jørgensen, Jesper Roland; Fransson, Anette; Fjord-Larsen, Lone; Thompson, Lachlan H; Houchins, Jeffrey P; Andrade, Nuno; Torp, Malene; Kalkkinen, Nisse; Andersson, Elisabet; Lindvall, Olle; Ulfendahl, Mats; Brunak, Søren; Johansen, Teit E; Wahlberg, Lars U.

In: Experimental Neurology, Vol. 233, No. 1, 2012, p. 172-81.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, JR, Fransson, A, Fjord-Larsen, L, Thompson, LH, Houchins, JP, Andrade, N, Torp, M, Kalkkinen, N, Andersson, E, Lindvall, O, Ulfendahl, M, Brunak, S, Johansen, TE & Wahlberg, LU 2012, 'Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo', Experimental Neurology, vol. 233, no. 1, pp. 172-81. https://doi.org/10.1016/j.expneurol.2011.09.027

APA

Jørgensen, J. R., Fransson, A., Fjord-Larsen, L., Thompson, L. H., Houchins, J. P., Andrade, N., Torp, M., Kalkkinen, N., Andersson, E., Lindvall, O., Ulfendahl, M., Brunak, S., Johansen, T. E., & Wahlberg, L. U. (2012). Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo. Experimental Neurology, 233(1), 172-81. https://doi.org/10.1016/j.expneurol.2011.09.027

Vancouver

Jørgensen JR, Fransson A, Fjord-Larsen L, Thompson LH, Houchins JP, Andrade N et al. Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo. Experimental Neurology. 2012;233(1):172-81. https://doi.org/10.1016/j.expneurol.2011.09.027

Author

Jørgensen, Jesper Roland ; Fransson, Anette ; Fjord-Larsen, Lone ; Thompson, Lachlan H ; Houchins, Jeffrey P ; Andrade, Nuno ; Torp, Malene ; Kalkkinen, Nisse ; Andersson, Elisabet ; Lindvall, Olle ; Ulfendahl, Mats ; Brunak, Søren ; Johansen, Teit E ; Wahlberg, Lars U. / Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo. In: Experimental Neurology. 2012 ; Vol. 233, No. 1. pp. 172-81.

Bibtex

@article{e446602edf9949778139c34f4f7efd93,
title = "Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo",
abstract = "Neurotrophic factors are secreted proteins responsible for migration, growth and survival of neurons during development, and for maintenance and plasticity of adult neurons. Here we present a novel secreted protein named Cometin which together with Meteorin defines a new evolutionary conserved protein family. During early mouse development, Cometin is found exclusively in the floor plate and from E13.5 also in dorsal root ganglions and inner ear but apparently not in the adult nervous system. In vitro, Cometin promotes neurite outgrowth from dorsal root ganglion cells which can be blocked by inhibition of the Janus or MEK kinases. In this assay, additive effects of Cometin and Meteorin are observed indicating separate receptors. Furthermore, Cometin supports migration of neuroblasts from subventricular zone explants to the same extend as stromal cell derived factor 1a. Given the neurotrophic properties in vitro, combined with the restricted inner ear expression during development, we further investigated Cometin in relation to deafness. In neomycin deafened guinea pigs, two weeks intracochlear infusion of recombinant Cometin supports spiral ganglion neuron survival and function. In contrast to the control group receiving artificial perilymph, Cometin treated animals retain normal electrically-evoked brainstem response which is maintained several weeks after treatment cessation. Neuroprotection is also evident from stereological analysis of the spiral ganglion. Altogether, these studies show that Cometin is a potent new neurotrophic factor with therapeutic potential.",
keywords = "Amino Acid Sequence, Animals, Animals, Newborn, Cell Movement, Cell Survival, Cells, Cultured, Central Nervous System, Cerebral Ventricles, Chromatography, High Pressure Liquid, Cloning, Molecular, Culture Media, Conditioned, Deafness, Disease Models, Animal, Dose-Response Relationship, Drug, Embryo, Mammalian, Enzyme Inhibitors, Female, Gene Expression Regulation, Developmental, Guinea Pigs, Humans, Male, Mice, Microscopy, Electron, Scanning, Microtubule-Associated Proteins, Neomycin, Nerve Growth Factors, Nerve Tissue Proteins, Neural Stem Cells, Neurites, Neurons, Neuropeptides, Rats, Spiral Ganglion, Tandem Mass Spectrometry, Transfection",
author = "J{\o}rgensen, {Jesper Roland} and Anette Fransson and Lone Fjord-Larsen and Thompson, {Lachlan H} and Houchins, {Jeffrey P} and Nuno Andrade and Malene Torp and Nisse Kalkkinen and Elisabet Andersson and Olle Lindvall and Mats Ulfendahl and S{\o}ren Brunak and Johansen, {Teit E} and Wahlberg, {Lars U}",
note = "Copyright {\textcopyright} 2011 Elsevier Inc. All rights reserved.",
year = "2012",
doi = "10.1016/j.expneurol.2011.09.027",
language = "English",
volume = "233",
pages = "172--81",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo

AU - Jørgensen, Jesper Roland

AU - Fransson, Anette

AU - Fjord-Larsen, Lone

AU - Thompson, Lachlan H

AU - Houchins, Jeffrey P

AU - Andrade, Nuno

AU - Torp, Malene

AU - Kalkkinen, Nisse

AU - Andersson, Elisabet

AU - Lindvall, Olle

AU - Ulfendahl, Mats

AU - Brunak, Søren

AU - Johansen, Teit E

AU - Wahlberg, Lars U

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Neurotrophic factors are secreted proteins responsible for migration, growth and survival of neurons during development, and for maintenance and plasticity of adult neurons. Here we present a novel secreted protein named Cometin which together with Meteorin defines a new evolutionary conserved protein family. During early mouse development, Cometin is found exclusively in the floor plate and from E13.5 also in dorsal root ganglions and inner ear but apparently not in the adult nervous system. In vitro, Cometin promotes neurite outgrowth from dorsal root ganglion cells which can be blocked by inhibition of the Janus or MEK kinases. In this assay, additive effects of Cometin and Meteorin are observed indicating separate receptors. Furthermore, Cometin supports migration of neuroblasts from subventricular zone explants to the same extend as stromal cell derived factor 1a. Given the neurotrophic properties in vitro, combined with the restricted inner ear expression during development, we further investigated Cometin in relation to deafness. In neomycin deafened guinea pigs, two weeks intracochlear infusion of recombinant Cometin supports spiral ganglion neuron survival and function. In contrast to the control group receiving artificial perilymph, Cometin treated animals retain normal electrically-evoked brainstem response which is maintained several weeks after treatment cessation. Neuroprotection is also evident from stereological analysis of the spiral ganglion. Altogether, these studies show that Cometin is a potent new neurotrophic factor with therapeutic potential.

AB - Neurotrophic factors are secreted proteins responsible for migration, growth and survival of neurons during development, and for maintenance and plasticity of adult neurons. Here we present a novel secreted protein named Cometin which together with Meteorin defines a new evolutionary conserved protein family. During early mouse development, Cometin is found exclusively in the floor plate and from E13.5 also in dorsal root ganglions and inner ear but apparently not in the adult nervous system. In vitro, Cometin promotes neurite outgrowth from dorsal root ganglion cells which can be blocked by inhibition of the Janus or MEK kinases. In this assay, additive effects of Cometin and Meteorin are observed indicating separate receptors. Furthermore, Cometin supports migration of neuroblasts from subventricular zone explants to the same extend as stromal cell derived factor 1a. Given the neurotrophic properties in vitro, combined with the restricted inner ear expression during development, we further investigated Cometin in relation to deafness. In neomycin deafened guinea pigs, two weeks intracochlear infusion of recombinant Cometin supports spiral ganglion neuron survival and function. In contrast to the control group receiving artificial perilymph, Cometin treated animals retain normal electrically-evoked brainstem response which is maintained several weeks after treatment cessation. Neuroprotection is also evident from stereological analysis of the spiral ganglion. Altogether, these studies show that Cometin is a potent new neurotrophic factor with therapeutic potential.

KW - Amino Acid Sequence

KW - Animals

KW - Animals, Newborn

KW - Cell Movement

KW - Cell Survival

KW - Cells, Cultured

KW - Central Nervous System

KW - Cerebral Ventricles

KW - Chromatography, High Pressure Liquid

KW - Cloning, Molecular

KW - Culture Media, Conditioned

KW - Deafness

KW - Disease Models, Animal

KW - Dose-Response Relationship, Drug

KW - Embryo, Mammalian

KW - Enzyme Inhibitors

KW - Female

KW - Gene Expression Regulation, Developmental

KW - Guinea Pigs

KW - Humans

KW - Male

KW - Mice

KW - Microscopy, Electron, Scanning

KW - Microtubule-Associated Proteins

KW - Neomycin

KW - Nerve Growth Factors

KW - Nerve Tissue Proteins

KW - Neural Stem Cells

KW - Neurites

KW - Neurons

KW - Neuropeptides

KW - Rats

KW - Spiral Ganglion

KW - Tandem Mass Spectrometry

KW - Transfection

U2 - 10.1016/j.expneurol.2011.09.027

DO - 10.1016/j.expneurol.2011.09.027

M3 - Journal article

C2 - 21985865

VL - 233

SP - 172

EP - 181

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 1

ER -

ID: 40804197