Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins. / Neufeld-Cohen, Adi; Robles, Maria S; Aviram, Rona; Manella, Gal; Adamovich, Yaarit; Ladeuix, Benjamin; Nir, Dana; Rousso-Noori, Liat; Kuperman, Yael; Golik, Marina; Mann, Matthias; Asher, Gad.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 12, 22.03.2016, p. E1673-82.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Neufeld-Cohen, A, Robles, MS, Aviram, R, Manella, G, Adamovich, Y, Ladeuix, B, Nir, D, Rousso-Noori, L, Kuperman, Y, Golik, M, Mann, M & Asher, G 2016, 'Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 12, pp. E1673-82. https://doi.org/10.1073/pnas.1519650113

APA

Neufeld-Cohen, A., Robles, M. S., Aviram, R., Manella, G., Adamovich, Y., Ladeuix, B., Nir, D., Rousso-Noori, L., Kuperman, Y., Golik, M., Mann, M., & Asher, G. (2016). Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins. Proceedings of the National Academy of Sciences of the United States of America, 113(12), E1673-82. https://doi.org/10.1073/pnas.1519650113

Vancouver

Neufeld-Cohen A, Robles MS, Aviram R, Manella G, Adamovich Y, Ladeuix B et al. Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins. Proceedings of the National Academy of Sciences of the United States of America. 2016 Mar 22;113(12):E1673-82. https://doi.org/10.1073/pnas.1519650113

Author

Neufeld-Cohen, Adi ; Robles, Maria S ; Aviram, Rona ; Manella, Gal ; Adamovich, Yaarit ; Ladeuix, Benjamin ; Nir, Dana ; Rousso-Noori, Liat ; Kuperman, Yael ; Golik, Marina ; Mann, Matthias ; Asher, Gad. / Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 12. pp. E1673-82.

Bibtex

@article{27394d9488484b8dbd2f14470174f812,
title = "Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins",
abstract = "Mitochondria are major suppliers of cellular energy through nutrients oxidation. Little is known about the mechanisms that enable mitochondria to cope with changes in nutrient supply and energy demand that naturally occur throughout the day. To address this question, we applied MS-based quantitative proteomics on isolated mitochondria from mice killed throughout the day and identified extensive oscillations in the mitochondrial proteome. Remarkably, the majority of cycling mitochondrial proteins peaked during the early light phase. We found that rate-limiting mitochondrial enzymes that process lipids and carbohydrates accumulate in a diurnal manner and are dependent on the clock proteins PER1/2. In this conjuncture, we uncovered daily oscillations in mitochondrial respiration that peak during different times of the day in response to different nutrients. Notably, the diurnal regulation of mitochondrial respiration was blunted in mice lacking PER1/2 or on a high-fat diet. We propose that PERIOD proteins optimize mitochondrial metabolism to daily changes in energy supply/demand and thereby, serve as a rheostat for mitochondrial nutrient utilization.",
keywords = "Animals, Circadian Rhythm, Citric Acid Cycle, Diet, High-Fat, Dietary Fats, Electron Transport, Fatty Acids, Feeding Behavior, Gene Expression Profiling, Male, Mice, Mice, Knockout, Mitochondria, Liver, Mitochondrial Proteins, Motor Activity, Period Circadian Proteins, Proteome, RNA, Messenger, Journal Article, Research Support, Non-U.S. Gov't",
author = "Adi Neufeld-Cohen and Robles, {Maria S} and Rona Aviram and Gal Manella and Yaarit Adamovich and Benjamin Ladeuix and Dana Nir and Liat Rousso-Noori and Yael Kuperman and Marina Golik and Matthias Mann and Gad Asher",
year = "2016",
month = mar,
day = "22",
doi = "10.1073/pnas.1519650113",
language = "English",
volume = "113",
pages = "E1673--82",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "12",

}

RIS

TY - JOUR

T1 - Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins

AU - Neufeld-Cohen, Adi

AU - Robles, Maria S

AU - Aviram, Rona

AU - Manella, Gal

AU - Adamovich, Yaarit

AU - Ladeuix, Benjamin

AU - Nir, Dana

AU - Rousso-Noori, Liat

AU - Kuperman, Yael

AU - Golik, Marina

AU - Mann, Matthias

AU - Asher, Gad

PY - 2016/3/22

Y1 - 2016/3/22

N2 - Mitochondria are major suppliers of cellular energy through nutrients oxidation. Little is known about the mechanisms that enable mitochondria to cope with changes in nutrient supply and energy demand that naturally occur throughout the day. To address this question, we applied MS-based quantitative proteomics on isolated mitochondria from mice killed throughout the day and identified extensive oscillations in the mitochondrial proteome. Remarkably, the majority of cycling mitochondrial proteins peaked during the early light phase. We found that rate-limiting mitochondrial enzymes that process lipids and carbohydrates accumulate in a diurnal manner and are dependent on the clock proteins PER1/2. In this conjuncture, we uncovered daily oscillations in mitochondrial respiration that peak during different times of the day in response to different nutrients. Notably, the diurnal regulation of mitochondrial respiration was blunted in mice lacking PER1/2 or on a high-fat diet. We propose that PERIOD proteins optimize mitochondrial metabolism to daily changes in energy supply/demand and thereby, serve as a rheostat for mitochondrial nutrient utilization.

AB - Mitochondria are major suppliers of cellular energy through nutrients oxidation. Little is known about the mechanisms that enable mitochondria to cope with changes in nutrient supply and energy demand that naturally occur throughout the day. To address this question, we applied MS-based quantitative proteomics on isolated mitochondria from mice killed throughout the day and identified extensive oscillations in the mitochondrial proteome. Remarkably, the majority of cycling mitochondrial proteins peaked during the early light phase. We found that rate-limiting mitochondrial enzymes that process lipids and carbohydrates accumulate in a diurnal manner and are dependent on the clock proteins PER1/2. In this conjuncture, we uncovered daily oscillations in mitochondrial respiration that peak during different times of the day in response to different nutrients. Notably, the diurnal regulation of mitochondrial respiration was blunted in mice lacking PER1/2 or on a high-fat diet. We propose that PERIOD proteins optimize mitochondrial metabolism to daily changes in energy supply/demand and thereby, serve as a rheostat for mitochondrial nutrient utilization.

KW - Animals

KW - Circadian Rhythm

KW - Citric Acid Cycle

KW - Diet, High-Fat

KW - Dietary Fats

KW - Electron Transport

KW - Fatty Acids

KW - Feeding Behavior

KW - Gene Expression Profiling

KW - Male

KW - Mice

KW - Mice, Knockout

KW - Mitochondria, Liver

KW - Mitochondrial Proteins

KW - Motor Activity

KW - Period Circadian Proteins

KW - Proteome

KW - RNA, Messenger

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1073/pnas.1519650113

DO - 10.1073/pnas.1519650113

M3 - Journal article

C2 - 26862173

VL - 113

SP - E1673-82

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 12

ER -

ID: 186877445