BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. / Feng, Yunpeng; Vlassis, Arsenios; Roques, Céline; Lalonde, Marie Eve; Gonzalez Aguilera, Cristina; Lambert, Jean Philippe; Lee, Sung-Bau; Zhao, Xiaobei; Alabert, Constance; Johansen, Jens Vilstrup; Paquet, Eric; Yang, Xiang Jiao; Gingras, Anne Claude; Côté, Jacques; Groth, Anja.

In: EMBO Journal, Vol. 35, No. 2, 2016, p. 176-192.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Feng, Y, Vlassis, A, Roques, C, Lalonde, ME, Gonzalez Aguilera, C, Lambert, JP, Lee, S-B, Zhao, X, Alabert, C, Johansen, JV, Paquet, E, Yang, XJ, Gingras, AC, Côté, J & Groth, A 2016, 'BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation', EMBO Journal, vol. 35, no. 2, pp. 176-192. https://doi.org/10.15252/embj.201591293

APA

Feng, Y., Vlassis, A., Roques, C., Lalonde, M. E., Gonzalez Aguilera, C., Lambert, J. P., Lee, S-B., Zhao, X., Alabert, C., Johansen, J. V., Paquet, E., Yang, X. J., Gingras, A. C., Côté, J., & Groth, A. (2016). BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. EMBO Journal, 35(2), 176-192. https://doi.org/10.15252/embj.201591293

Vancouver

Feng Y, Vlassis A, Roques C, Lalonde ME, Gonzalez Aguilera C, Lambert JP et al. BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. EMBO Journal. 2016;35(2):176-192. https://doi.org/10.15252/embj.201591293

Author

Feng, Yunpeng ; Vlassis, Arsenios ; Roques, Céline ; Lalonde, Marie Eve ; Gonzalez Aguilera, Cristina ; Lambert, Jean Philippe ; Lee, Sung-Bau ; Zhao, Xiaobei ; Alabert, Constance ; Johansen, Jens Vilstrup ; Paquet, Eric ; Yang, Xiang Jiao ; Gingras, Anne Claude ; Côté, Jacques ; Groth, Anja. / BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. In: EMBO Journal. 2016 ; Vol. 35, No. 2. pp. 176-192.

Bibtex

@article{13230432256c44c1b4bd5dd4c2e1dfeb,
title = "BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation",
abstract = "During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators implicated in replication control by measuring RPA accumulation upon replication stress. We identify six factors required for normal rates of DNA replication and characterize a function of the bromodomain and PHD finger-containing protein 3 (BRPF3) in replication initiation. BRPF3 forms a complex with HBO1 that specifically acetylates histone H3K14, and genomewide analysis shows high enrichment of BRPF3, HBO1 and H3K14ac at ORC1-binding sites and replication origins found in the vicinity of TSSs. Consistent with this, BRPF3 is necessary for H3K14ac at selected origins and efficient origin activation. CDC45 recruitment, but not MCM2-7 loading, is impaired in BRPF3-depleted cells, identifying a BRPF3-dependent function of HBO1 in origin activation that is complementary to its role in licencing. We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication origins.",
keywords = "BRPF, DNA replication, H3K14ac, HBO1, Origin activation",
author = "Yunpeng Feng and Arsenios Vlassis and C{\'e}line Roques and Lalonde, {Marie Eve} and {Gonzalez Aguilera}, Cristina and Lambert, {Jean Philippe} and Sung-Bau Lee and Xiaobei Zhao and Constance Alabert and Johansen, {Jens Vilstrup} and Eric Paquet and Yang, {Xiang Jiao} and Gingras, {Anne Claude} and Jacques C{\^o}t{\'e} and Anja Groth",
year = "2016",
doi = "10.15252/embj.201591293",
language = "English",
volume = "35",
pages = "176--192",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation

AU - Feng, Yunpeng

AU - Vlassis, Arsenios

AU - Roques, Céline

AU - Lalonde, Marie Eve

AU - Gonzalez Aguilera, Cristina

AU - Lambert, Jean Philippe

AU - Lee, Sung-Bau

AU - Zhao, Xiaobei

AU - Alabert, Constance

AU - Johansen, Jens Vilstrup

AU - Paquet, Eric

AU - Yang, Xiang Jiao

AU - Gingras, Anne Claude

AU - Côté, Jacques

AU - Groth, Anja

PY - 2016

Y1 - 2016

N2 - During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators implicated in replication control by measuring RPA accumulation upon replication stress. We identify six factors required for normal rates of DNA replication and characterize a function of the bromodomain and PHD finger-containing protein 3 (BRPF3) in replication initiation. BRPF3 forms a complex with HBO1 that specifically acetylates histone H3K14, and genomewide analysis shows high enrichment of BRPF3, HBO1 and H3K14ac at ORC1-binding sites and replication origins found in the vicinity of TSSs. Consistent with this, BRPF3 is necessary for H3K14ac at selected origins and efficient origin activation. CDC45 recruitment, but not MCM2-7 loading, is impaired in BRPF3-depleted cells, identifying a BRPF3-dependent function of HBO1 in origin activation that is complementary to its role in licencing. We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication origins.

AB - During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators implicated in replication control by measuring RPA accumulation upon replication stress. We identify six factors required for normal rates of DNA replication and characterize a function of the bromodomain and PHD finger-containing protein 3 (BRPF3) in replication initiation. BRPF3 forms a complex with HBO1 that specifically acetylates histone H3K14, and genomewide analysis shows high enrichment of BRPF3, HBO1 and H3K14ac at ORC1-binding sites and replication origins found in the vicinity of TSSs. Consistent with this, BRPF3 is necessary for H3K14ac at selected origins and efficient origin activation. CDC45 recruitment, but not MCM2-7 loading, is impaired in BRPF3-depleted cells, identifying a BRPF3-dependent function of HBO1 in origin activation that is complementary to its role in licencing. We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication origins.

KW - BRPF

KW - DNA replication

KW - H3K14ac

KW - HBO1

KW - Origin activation

U2 - 10.15252/embj.201591293

DO - 10.15252/embj.201591293

M3 - Journal article

C2 - 26620551

VL - 35

SP - 176

EP - 192

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 2

ER -

ID: 153413621