A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment
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A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment. / Bernard, Clément; Locard-Paulet, Marie; Noël, Cyril; Duchateau, Magalie; Giai Gianetto, Quentin; Moumen, Bouziane; Rattei, Thomas; Hechard, Yann; Jensen, Lars Juhl; Matondo, Mariette; Samba-Louaka, Ascel.
In: Nature Communications, Vol. 13, No. 1, 4104, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment
AU - Bernard, Clément
AU - Locard-Paulet, Marie
AU - Noël, Cyril
AU - Duchateau, Magalie
AU - Giai Gianetto, Quentin
AU - Moumen, Bouziane
AU - Rattei, Thomas
AU - Hechard, Yann
AU - Jensen, Lars Juhl
AU - Matondo, Mariette
AU - Samba-Louaka, Ascel
N1 - Publisher Copyright: © 2022, The Author(s).
PY - 2022
Y1 - 2022
N2 - Encystment is a common stress response of most protists, including free-living amoebae. Cyst formation protects the amoebae from eradication and can increase virulence of the bacteria they harbor. Here, we mapped the global molecular changes that occur in the facultatively pathogenic amoeba Acanthamoeba castellanii during the early steps of the poorly understood process of encystment. By performing transcriptomic, proteomic, and phosphoproteomic experiments during encystment, we identified more than 150,000 previously undescribed transcripts and thousands of protein sequences absent from the reference genome. These results provide molecular details to the regulation of expected biological processes, such as cell proliferation shutdown, and reveal new insights such as a rapid phospho-regulation of sites involved in cytoskeleton remodeling and translation regulation. This work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource for further investigation of amoebae encystment to allow for a better control of pathogenic amoebae.
AB - Encystment is a common stress response of most protists, including free-living amoebae. Cyst formation protects the amoebae from eradication and can increase virulence of the bacteria they harbor. Here, we mapped the global molecular changes that occur in the facultatively pathogenic amoeba Acanthamoeba castellanii during the early steps of the poorly understood process of encystment. By performing transcriptomic, proteomic, and phosphoproteomic experiments during encystment, we identified more than 150,000 previously undescribed transcripts and thousands of protein sequences absent from the reference genome. These results provide molecular details to the regulation of expected biological processes, such as cell proliferation shutdown, and reveal new insights such as a rapid phospho-regulation of sites involved in cytoskeleton remodeling and translation regulation. This work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource for further investigation of amoebae encystment to allow for a better control of pathogenic amoebae.
U2 - 10.1038/s41467-022-31832-0
DO - 10.1038/s41467-022-31832-0
M3 - Journal article
C2 - 35835784
AN - SCOPUS:85134145889
VL - 13
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 4104
ER -
ID: 314631161