A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. / Bassik, Michael C; Kampmann, Martin; Lebbink, Robert Jan; Wang, Shuyi; Hein, Marco Y; Poser, Ina; Weibezahn, Jimena; Horlbeck, Max A; Chen, Siyuan; Mann, Matthias; Hyman, Anthony A; Leproust, Emily M; McManus, Michael T; Weissman, Jonathan S.

In: Cell, Vol. 152, No. 4, 14.02.2013, p. 909-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bassik, MC, Kampmann, M, Lebbink, RJ, Wang, S, Hein, MY, Poser, I, Weibezahn, J, Horlbeck, MA, Chen, S, Mann, M, Hyman, AA, Leproust, EM, McManus, MT & Weissman, JS 2013, 'A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility', Cell, vol. 152, no. 4, pp. 909-22. https://doi.org/10.1016/j.cell.2013.01.030

APA

Bassik, M. C., Kampmann, M., Lebbink, R. J., Wang, S., Hein, M. Y., Poser, I., Weibezahn, J., Horlbeck, M. A., Chen, S., Mann, M., Hyman, A. A., Leproust, E. M., McManus, M. T., & Weissman, J. S. (2013). A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. Cell, 152(4), 909-22. https://doi.org/10.1016/j.cell.2013.01.030

Vancouver

Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I et al. A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. Cell. 2013 Feb 14;152(4):909-22. https://doi.org/10.1016/j.cell.2013.01.030

Author

Bassik, Michael C ; Kampmann, Martin ; Lebbink, Robert Jan ; Wang, Shuyi ; Hein, Marco Y ; Poser, Ina ; Weibezahn, Jimena ; Horlbeck, Max A ; Chen, Siyuan ; Mann, Matthias ; Hyman, Anthony A ; Leproust, Emily M ; McManus, Michael T ; Weissman, Jonathan S. / A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. In: Cell. 2013 ; Vol. 152, No. 4. pp. 909-22.

Bibtex

@article{ccb6c9d21bab44f388267a74c8450483,
title = "A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility",
abstract = "Genetic interaction (GI) maps, comprising pairwise measures of how strongly the function of one gene depends on the presence of a second, have enabled the systematic exploration of gene function in microorganisms. Here, we present a two-stage strategy to construct high-density GI maps in mammalian cells. First, we use ultracomplex pooled shRNA libraries (25 shRNAs/gene) to identify high-confidence hit genes for a given phenotype and effective shRNAs. We then construct double-shRNA libraries from these to systematically measure GIs between hits. A GI map focused on ricin susceptibility broadly recapitulates known pathways and provides many unexpected insights. These include a noncanonical role for COPI, a previously uncharacterized protein complex affecting toxin clearance, a specialized role for the ribosomal protein RPS25, and functionally distinct mammalian TRAPP complexes. The ability to rapidly generate mammalian GI maps provides a potentially transformative tool for defining gene function and designing combination therapies based on synergistic pairs.",
keywords = "Biological Transport, Carrier Proteins, Cell Line, Tumor, Coat Protein Complex I, Endoplasmic Reticulum, Epistasis, Genetic, Heptanoic Acids, Humans, Membrane Proteins, Proto-Oncogene Proteins, Pyrroles, RNA, Small Interfering, Ribosomal Proteins, Ricin, Vesicular Transport Proteins",
author = "Bassik, {Michael C} and Martin Kampmann and Lebbink, {Robert Jan} and Shuyi Wang and Hein, {Marco Y} and Ina Poser and Jimena Weibezahn and Horlbeck, {Max A} and Siyuan Chen and Matthias Mann and Hyman, {Anthony A} and Leproust, {Emily M} and McManus, {Michael T} and Weissman, {Jonathan S}",
note = "Copyright {\textcopyright} 2013 Elsevier Inc. All rights reserved.",
year = "2013",
month = feb,
day = "14",
doi = "10.1016/j.cell.2013.01.030",
language = "English",
volume = "152",
pages = "909--22",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility

AU - Bassik, Michael C

AU - Kampmann, Martin

AU - Lebbink, Robert Jan

AU - Wang, Shuyi

AU - Hein, Marco Y

AU - Poser, Ina

AU - Weibezahn, Jimena

AU - Horlbeck, Max A

AU - Chen, Siyuan

AU - Mann, Matthias

AU - Hyman, Anthony A

AU - Leproust, Emily M

AU - McManus, Michael T

AU - Weissman, Jonathan S

N1 - Copyright © 2013 Elsevier Inc. All rights reserved.

PY - 2013/2/14

Y1 - 2013/2/14

N2 - Genetic interaction (GI) maps, comprising pairwise measures of how strongly the function of one gene depends on the presence of a second, have enabled the systematic exploration of gene function in microorganisms. Here, we present a two-stage strategy to construct high-density GI maps in mammalian cells. First, we use ultracomplex pooled shRNA libraries (25 shRNAs/gene) to identify high-confidence hit genes for a given phenotype and effective shRNAs. We then construct double-shRNA libraries from these to systematically measure GIs between hits. A GI map focused on ricin susceptibility broadly recapitulates known pathways and provides many unexpected insights. These include a noncanonical role for COPI, a previously uncharacterized protein complex affecting toxin clearance, a specialized role for the ribosomal protein RPS25, and functionally distinct mammalian TRAPP complexes. The ability to rapidly generate mammalian GI maps provides a potentially transformative tool for defining gene function and designing combination therapies based on synergistic pairs.

AB - Genetic interaction (GI) maps, comprising pairwise measures of how strongly the function of one gene depends on the presence of a second, have enabled the systematic exploration of gene function in microorganisms. Here, we present a two-stage strategy to construct high-density GI maps in mammalian cells. First, we use ultracomplex pooled shRNA libraries (25 shRNAs/gene) to identify high-confidence hit genes for a given phenotype and effective shRNAs. We then construct double-shRNA libraries from these to systematically measure GIs between hits. A GI map focused on ricin susceptibility broadly recapitulates known pathways and provides many unexpected insights. These include a noncanonical role for COPI, a previously uncharacterized protein complex affecting toxin clearance, a specialized role for the ribosomal protein RPS25, and functionally distinct mammalian TRAPP complexes. The ability to rapidly generate mammalian GI maps provides a potentially transformative tool for defining gene function and designing combination therapies based on synergistic pairs.

KW - Biological Transport

KW - Carrier Proteins

KW - Cell Line, Tumor

KW - Coat Protein Complex I

KW - Endoplasmic Reticulum

KW - Epistasis, Genetic

KW - Heptanoic Acids

KW - Humans

KW - Membrane Proteins

KW - Proto-Oncogene Proteins

KW - Pyrroles

KW - RNA, Small Interfering

KW - Ribosomal Proteins

KW - Ricin

KW - Vesicular Transport Proteins

U2 - 10.1016/j.cell.2013.01.030

DO - 10.1016/j.cell.2013.01.030

M3 - Journal article

C2 - 23394947

VL - 152

SP - 909

EP - 922

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -

ID: 88589714