A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation. / Zhang, Gang; Lischetti, Tiziana; Nilsson, Jakob.

In: Journal of Cell Science, Vol. 127, 20.12.2013, p. 871-884.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, G, Lischetti, T & Nilsson, J 2013, 'A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation', Journal of Cell Science, vol. 127, pp. 871-884. https://doi.org/10.1242/jcs.139725

APA

Zhang, G., Lischetti, T., & Nilsson, J. (2013). A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation. Journal of Cell Science, 127, 871-884. https://doi.org/10.1242/jcs.139725

Vancouver

Zhang G, Lischetti T, Nilsson J. A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation. Journal of Cell Science. 2013 Dec 20;127:871-884. https://doi.org/10.1242/jcs.139725

Author

Zhang, Gang ; Lischetti, Tiziana ; Nilsson, Jakob. / A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation. In: Journal of Cell Science. 2013 ; Vol. 127. pp. 871-884.

Bibtex

@article{725a19be9bee4bae8ca75cff49d80da0,
title = "A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation",
abstract = "The Bub1-Bub3 and BubR1-Bub3 checkpoint complexes, or the Bubs, contribute to the accurate segregation of chromosomes during mitosis by promoting chromosome bi-orientation and halting exit from mitosis if this fails. The complexes associate with kinetochores during mitosis, which is required for proper chromosome segregation. The outer kinetochore protein KNL1 (also known as CASC5/Blinkin/AF15Q14) is the receptor for Bub proteins but the exact nature of the functional binding sites on KNL1 are yet to be determined. Here, we show that KNL1 contains multiple binding sites for the Bub proteins, with the Mps1-phosphorylated MELT repeats constituting individual functional docking sites for direct binding of Bub3. Surprisingly, chromosome congression and the Spindle Assembly Checkpoint (SAC) are still functional when KNL1 is deleted of all but four of its twelve MELT repeats. Systematically reducing the number of MELT repeats to less than four reduced KNL1 functionality. Furthermore, we show that Protein Phosphatase 1 (PP1) binding to KNL1 in prometaphase reduces the levels of Bub proteins at kinetochores to approximately the level recruited by four active MELT repeats.",
author = "Gang Zhang and Tiziana Lischetti and Jakob Nilsson",
year = "2013",
month = dec,
day = "20",
doi = "10.1242/jcs.139725",
language = "English",
volume = "127",
pages = "871--884",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",

}

RIS

TY - JOUR

T1 - A minimal number of MELT repeats supports all functions of KNL1 in chromosome segregation

AU - Zhang, Gang

AU - Lischetti, Tiziana

AU - Nilsson, Jakob

PY - 2013/12/20

Y1 - 2013/12/20

N2 - The Bub1-Bub3 and BubR1-Bub3 checkpoint complexes, or the Bubs, contribute to the accurate segregation of chromosomes during mitosis by promoting chromosome bi-orientation and halting exit from mitosis if this fails. The complexes associate with kinetochores during mitosis, which is required for proper chromosome segregation. The outer kinetochore protein KNL1 (also known as CASC5/Blinkin/AF15Q14) is the receptor for Bub proteins but the exact nature of the functional binding sites on KNL1 are yet to be determined. Here, we show that KNL1 contains multiple binding sites for the Bub proteins, with the Mps1-phosphorylated MELT repeats constituting individual functional docking sites for direct binding of Bub3. Surprisingly, chromosome congression and the Spindle Assembly Checkpoint (SAC) are still functional when KNL1 is deleted of all but four of its twelve MELT repeats. Systematically reducing the number of MELT repeats to less than four reduced KNL1 functionality. Furthermore, we show that Protein Phosphatase 1 (PP1) binding to KNL1 in prometaphase reduces the levels of Bub proteins at kinetochores to approximately the level recruited by four active MELT repeats.

AB - The Bub1-Bub3 and BubR1-Bub3 checkpoint complexes, or the Bubs, contribute to the accurate segregation of chromosomes during mitosis by promoting chromosome bi-orientation and halting exit from mitosis if this fails. The complexes associate with kinetochores during mitosis, which is required for proper chromosome segregation. The outer kinetochore protein KNL1 (also known as CASC5/Blinkin/AF15Q14) is the receptor for Bub proteins but the exact nature of the functional binding sites on KNL1 are yet to be determined. Here, we show that KNL1 contains multiple binding sites for the Bub proteins, with the Mps1-phosphorylated MELT repeats constituting individual functional docking sites for direct binding of Bub3. Surprisingly, chromosome congression and the Spindle Assembly Checkpoint (SAC) are still functional when KNL1 is deleted of all but four of its twelve MELT repeats. Systematically reducing the number of MELT repeats to less than four reduced KNL1 functionality. Furthermore, we show that Protein Phosphatase 1 (PP1) binding to KNL1 in prometaphase reduces the levels of Bub proteins at kinetochores to approximately the level recruited by four active MELT repeats.

U2 - 10.1242/jcs.139725

DO - 10.1242/jcs.139725

M3 - Journal article

C2 - 24363448

VL - 127

SP - 871

EP - 884

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -

ID: 97267773