A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis. / Ueki, Yumi; Hadders, Michael A; Weisser, Melanie B; Nasa, Isha; Sotelo-Parrilla, Paula; Cressey, Lauren E; Gupta, Tanmay; Hertz, Emil P T; Kruse, Thomas; Montoya, Guillermo; Jeyaprakash, A. Arockia; Kettenbach, Arminja; Lens, Susanne M A; Nilsson, Jakob.

In: EMBO Reports, Vol. 22, No. 7, e52295, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ueki, Y, Hadders, MA, Weisser, MB, Nasa, I, Sotelo-Parrilla, P, Cressey, LE, Gupta, T, Hertz, EPT, Kruse, T, Montoya, G, Jeyaprakash, AA, Kettenbach, A, Lens, SMA & Nilsson, J 2021, 'A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis', EMBO Reports, vol. 22, no. 7, e52295. https://doi.org/10.15252/embr.202052295

APA

Ueki, Y., Hadders, M. A., Weisser, M. B., Nasa, I., Sotelo-Parrilla, P., Cressey, L. E., Gupta, T., Hertz, E. P. T., Kruse, T., Montoya, G., Jeyaprakash, A. A., Kettenbach, A., Lens, S. M. A., & Nilsson, J. (2021). A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis. EMBO Reports, 22(7), [e52295]. https://doi.org/10.15252/embr.202052295

Vancouver

Ueki Y, Hadders MA, Weisser MB, Nasa I, Sotelo-Parrilla P, Cressey LE et al. A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis. EMBO Reports. 2021;22(7). e52295. https://doi.org/10.15252/embr.202052295

Author

Ueki, Yumi ; Hadders, Michael A ; Weisser, Melanie B ; Nasa, Isha ; Sotelo-Parrilla, Paula ; Cressey, Lauren E ; Gupta, Tanmay ; Hertz, Emil P T ; Kruse, Thomas ; Montoya, Guillermo ; Jeyaprakash, A. Arockia ; Kettenbach, Arminja ; Lens, Susanne M A ; Nilsson, Jakob. / A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis. In: EMBO Reports. 2021 ; Vol. 22, No. 7.

Bibtex

@article{14f5ce2c647d441f8ca723b6d951145b,
title = "A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis",
abstract = "The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled-coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis.",
author = "Yumi Ueki and Hadders, {Michael A} and Weisser, {Melanie B} and Isha Nasa and Paula Sotelo-Parrilla and Cressey, {Lauren E} and Tanmay Gupta and Hertz, {Emil P T} and Thomas Kruse and Guillermo Montoya and Jeyaprakash, {A. Arockia} and Arminja Kettenbach and Lens, {Susanne M A} and Jakob Nilsson",
year = "2021",
doi = "10.15252/embr.202052295",
language = "English",
volume = "22",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",
number = "7",

}

RIS

TY - JOUR

T1 - A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis

AU - Ueki, Yumi

AU - Hadders, Michael A

AU - Weisser, Melanie B

AU - Nasa, Isha

AU - Sotelo-Parrilla, Paula

AU - Cressey, Lauren E

AU - Gupta, Tanmay

AU - Hertz, Emil P T

AU - Kruse, Thomas

AU - Montoya, Guillermo

AU - Jeyaprakash, A. Arockia

AU - Kettenbach, Arminja

AU - Lens, Susanne M A

AU - Nilsson, Jakob

PY - 2021

Y1 - 2021

N2 - The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled-coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis.

AB - The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled-coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis.

U2 - 10.15252/embr.202052295

DO - 10.15252/embr.202052295

M3 - Journal article

C2 - 33973335

VL - 22

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 7

M1 - e52295

ER -

ID: 261517491