A genome-wide resource for the analysis of protein localisation in Drosophila

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A genome-wide resource for the analysis of protein localisation in Drosophila. / Sarov, Mihail; Barz, Christiane; Jambor, Helena; Hein, Marco Y; Schmied, Christopher; Suchold, Dana; Stender, Bettina; Janosch, Stephan; K J, Vinay Vikas; Krishnan, R T; Krishnamoorthy, Aishwarya; Ferreira, Irene R S; Ejsmont, Radoslaw K; Finkl, Katja; Hasse, Susanne; Kämpfer, Philipp; Plewka, Nicole; Vinis, Elisabeth; Schloissnig, Siegfried; Knust, Elisabeth; Hartenstein, Volker; Mann, Matthias; Ramaswami, Mani; VijayRaghavan, K; Tomancak, Pavel; Schnorrer, Frank.

In: eLife, Vol. 5, 20.02.2016, p. e12068.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sarov, M, Barz, C, Jambor, H, Hein, MY, Schmied, C, Suchold, D, Stender, B, Janosch, S, K J, VV, Krishnan, RT, Krishnamoorthy, A, Ferreira, IRS, Ejsmont, RK, Finkl, K, Hasse, S, Kämpfer, P, Plewka, N, Vinis, E, Schloissnig, S, Knust, E, Hartenstein, V, Mann, M, Ramaswami, M, VijayRaghavan, K, Tomancak, P & Schnorrer, F 2016, 'A genome-wide resource for the analysis of protein localisation in Drosophila', eLife, vol. 5, pp. e12068. https://doi.org/10.7554/eLife.12068

APA

Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., K J, V. V., Krishnan, R. T., Krishnamoorthy, A., Ferreira, I. R. S., Ejsmont, R. K., Finkl, K., Hasse, S., Kämpfer, P., Plewka, N., Vinis, E., Schloissnig, S., ... Schnorrer, F. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila. eLife, 5, e12068. https://doi.org/10.7554/eLife.12068

Vancouver

Sarov M, Barz C, Jambor H, Hein MY, Schmied C, Suchold D et al. A genome-wide resource for the analysis of protein localisation in Drosophila. eLife. 2016 Feb 20;5:e12068. https://doi.org/10.7554/eLife.12068

Author

Sarov, Mihail ; Barz, Christiane ; Jambor, Helena ; Hein, Marco Y ; Schmied, Christopher ; Suchold, Dana ; Stender, Bettina ; Janosch, Stephan ; K J, Vinay Vikas ; Krishnan, R T ; Krishnamoorthy, Aishwarya ; Ferreira, Irene R S ; Ejsmont, Radoslaw K ; Finkl, Katja ; Hasse, Susanne ; Kämpfer, Philipp ; Plewka, Nicole ; Vinis, Elisabeth ; Schloissnig, Siegfried ; Knust, Elisabeth ; Hartenstein, Volker ; Mann, Matthias ; Ramaswami, Mani ; VijayRaghavan, K ; Tomancak, Pavel ; Schnorrer, Frank. / A genome-wide resource for the analysis of protein localisation in Drosophila. In: eLife. 2016 ; Vol. 5. pp. e12068.

Bibtex

@article{ca4d9f0c4dfc4ae68c3c4486855f1042,
title = "A genome-wide resource for the analysis of protein localisation in Drosophila",
abstract = "The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.",
keywords = "Animal Structures, Animals, Animals, Genetically Modified, Drosophila, Drosophila Proteins, Entomology, Gene Library, Genes, Reporter, Genome, Insect, Green Fluorescent Proteins, Image Processing, Computer-Assisted, Molecular Biology, Optical Imaging, Recombinant Fusion Proteins, Staining and Labeling, Journal Article, Research Support, Non-U.S. Gov't",
author = "Mihail Sarov and Christiane Barz and Helena Jambor and Hein, {Marco Y} and Christopher Schmied and Dana Suchold and Bettina Stender and Stephan Janosch and {K J}, {Vinay Vikas} and Krishnan, {R T} and Aishwarya Krishnamoorthy and Ferreira, {Irene R S} and Ejsmont, {Radoslaw K} and Katja Finkl and Susanne Hasse and Philipp K{\"a}mpfer and Nicole Plewka and Elisabeth Vinis and Siegfried Schloissnig and Elisabeth Knust and Volker Hartenstein and Matthias Mann and Mani Ramaswami and K VijayRaghavan and Pavel Tomancak and Frank Schnorrer",
year = "2016",
month = feb,
day = "20",
doi = "10.7554/eLife.12068",
language = "English",
volume = "5",
pages = "e12068",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - A genome-wide resource for the analysis of protein localisation in Drosophila

AU - Sarov, Mihail

AU - Barz, Christiane

AU - Jambor, Helena

AU - Hein, Marco Y

AU - Schmied, Christopher

AU - Suchold, Dana

AU - Stender, Bettina

AU - Janosch, Stephan

AU - K J, Vinay Vikas

AU - Krishnan, R T

AU - Krishnamoorthy, Aishwarya

AU - Ferreira, Irene R S

AU - Ejsmont, Radoslaw K

AU - Finkl, Katja

AU - Hasse, Susanne

AU - Kämpfer, Philipp

AU - Plewka, Nicole

AU - Vinis, Elisabeth

AU - Schloissnig, Siegfried

AU - Knust, Elisabeth

AU - Hartenstein, Volker

AU - Mann, Matthias

AU - Ramaswami, Mani

AU - VijayRaghavan, K

AU - Tomancak, Pavel

AU - Schnorrer, Frank

PY - 2016/2/20

Y1 - 2016/2/20

N2 - The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.

AB - The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.

KW - Animal Structures

KW - Animals

KW - Animals, Genetically Modified

KW - Drosophila

KW - Drosophila Proteins

KW - Entomology

KW - Gene Library

KW - Genes, Reporter

KW - Genome, Insect

KW - Green Fluorescent Proteins

KW - Image Processing, Computer-Assisted

KW - Molecular Biology

KW - Optical Imaging

KW - Recombinant Fusion Proteins

KW - Staining and Labeling

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.7554/eLife.12068

DO - 10.7554/eLife.12068

M3 - Journal article

C2 - 26896675

VL - 5

SP - e12068

JO - eLife

JF - eLife

SN - 2050-084X

ER -

ID: 186877335