A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment

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A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment. / Kruse, Thomas; Larsen, Marie Sofie Yoo; Sedgwick, Garry G; Sigurdsson, Jón Otti; Streicher, Werner; Olsen, Jesper Velgaard; Nilsson, Jakob.

In: E M B O Reports, Vol. 15, 29.01.2014, p. 282-290.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kruse, T, Larsen, MSY, Sedgwick, GG, Sigurdsson, JO, Streicher, W, Olsen, JV & Nilsson, J 2014, 'A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment', E M B O Reports, vol. 15, pp. 282-290. https://doi.org/10.1002/embr.201338101

APA

Kruse, T., Larsen, M. S. Y., Sedgwick, G. G., Sigurdsson, J. O., Streicher, W., Olsen, J. V., & Nilsson, J. (2014). A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment. E M B O Reports, 15, 282-290. https://doi.org/10.1002/embr.201338101

Vancouver

Kruse T, Larsen MSY, Sedgwick GG, Sigurdsson JO, Streicher W, Olsen JV et al. A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment. E M B O Reports. 2014 Jan 29;15:282-290. https://doi.org/10.1002/embr.201338101

Author

Kruse, Thomas ; Larsen, Marie Sofie Yoo ; Sedgwick, Garry G ; Sigurdsson, Jón Otti ; Streicher, Werner ; Olsen, Jesper Velgaard ; Nilsson, Jakob. / A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment. In: E M B O Reports. 2014 ; Vol. 15. pp. 282-290.

Bibtex

@article{a51b457700014c69ac80eeccca8fd77c,
title = "A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment",
abstract = "The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation by delaying entry into anaphase until all sister chromatids have become bi-oriented. A key component of the SAC is the Mad2 protein, which can adopt either an inactive open (O-Mad2) or active closed (C-Mad2) conformation. The conversion of O-Mad2 into C-Mad2 at unattached kinetochores is thought to be a key step in activating the SAC. The {"}template model{"} proposes that this is achieved by the recruitment of soluble O-Mad2 to C-Mad2 bound at kinetochores through its interaction with Mad1. Whether Mad1 has additional roles in the SAC beyond recruitment of C-Mad2 to kinetochores has not yet been addressed. Here, we show that Mad1 is required for mitotic arrest even when C-Mad2 is artificially recruited to kinetochores, indicating that it has indeed an additional function in promoting the checkpoint. The C-terminal globular domain of Mad1 and conserved residues in this region are required for this unexpected function of Mad1.",
author = "Thomas Kruse and Larsen, {Marie Sofie Yoo} and Sedgwick, {Garry G} and Sigurdsson, {J{\'o}n Otti} and Werner Streicher and Olsen, {Jesper Velgaard} and Jakob Nilsson",
year = "2014",
month = jan,
day = "29",
doi = "10.1002/embr.201338101",
language = "English",
volume = "15",
pages = "282--290",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment

AU - Kruse, Thomas

AU - Larsen, Marie Sofie Yoo

AU - Sedgwick, Garry G

AU - Sigurdsson, Jón Otti

AU - Streicher, Werner

AU - Olsen, Jesper Velgaard

AU - Nilsson, Jakob

PY - 2014/1/29

Y1 - 2014/1/29

N2 - The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation by delaying entry into anaphase until all sister chromatids have become bi-oriented. A key component of the SAC is the Mad2 protein, which can adopt either an inactive open (O-Mad2) or active closed (C-Mad2) conformation. The conversion of O-Mad2 into C-Mad2 at unattached kinetochores is thought to be a key step in activating the SAC. The "template model" proposes that this is achieved by the recruitment of soluble O-Mad2 to C-Mad2 bound at kinetochores through its interaction with Mad1. Whether Mad1 has additional roles in the SAC beyond recruitment of C-Mad2 to kinetochores has not yet been addressed. Here, we show that Mad1 is required for mitotic arrest even when C-Mad2 is artificially recruited to kinetochores, indicating that it has indeed an additional function in promoting the checkpoint. The C-terminal globular domain of Mad1 and conserved residues in this region are required for this unexpected function of Mad1.

AB - The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation by delaying entry into anaphase until all sister chromatids have become bi-oriented. A key component of the SAC is the Mad2 protein, which can adopt either an inactive open (O-Mad2) or active closed (C-Mad2) conformation. The conversion of O-Mad2 into C-Mad2 at unattached kinetochores is thought to be a key step in activating the SAC. The "template model" proposes that this is achieved by the recruitment of soluble O-Mad2 to C-Mad2 bound at kinetochores through its interaction with Mad1. Whether Mad1 has additional roles in the SAC beyond recruitment of C-Mad2 to kinetochores has not yet been addressed. Here, we show that Mad1 is required for mitotic arrest even when C-Mad2 is artificially recruited to kinetochores, indicating that it has indeed an additional function in promoting the checkpoint. The C-terminal globular domain of Mad1 and conserved residues in this region are required for this unexpected function of Mad1.

U2 - 10.1002/embr.201338101

DO - 10.1002/embr.201338101

M3 - Journal article

C2 - 24477933

VL - 15

SP - 282

EP - 290

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

ER -

ID: 102260550