A deeper look at carrier proteome effects for single-cell proteomics

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A deeper look at carrier proteome effects for single-cell proteomics. / Ye, Zilu; Batth, Tanveer S.; Rüther, Patrick; Olsen, Jesper V.

In: Communications Biology , Vol. 5, 150, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ye, Z, Batth, TS, Rüther, P & Olsen, JV 2022, 'A deeper look at carrier proteome effects for single-cell proteomics', Communications Biology , vol. 5, 150. https://doi.org/10.1038/s42003-022-03095-4

APA

Ye, Z., Batth, T. S., Rüther, P., & Olsen, J. V. (2022). A deeper look at carrier proteome effects for single-cell proteomics. Communications Biology , 5, [150]. https://doi.org/10.1038/s42003-022-03095-4

Vancouver

Ye Z, Batth TS, Rüther P, Olsen JV. A deeper look at carrier proteome effects for single-cell proteomics. Communications Biology . 2022;5. 150. https://doi.org/10.1038/s42003-022-03095-4

Author

Ye, Zilu ; Batth, Tanveer S. ; Rüther, Patrick ; Olsen, Jesper V. / A deeper look at carrier proteome effects for single-cell proteomics. In: Communications Biology . 2022 ; Vol. 5.

Bibtex

@article{d434d90046b645b8b3747cbd74156fbe,
title = "A deeper look at carrier proteome effects for single-cell proteomics",
abstract = "Multiplexing approaches using tandem mass tags with a carrier proteome to boost sensitivity have advanced single cell proteomics by mass spectrometry (SCoPE-MS). Here, we probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins. Guidelines for optimized mass spectrometry acquisition parameters and best practices for fold-change or protein copy number-based comparisons are provided.",
author = "Zilu Ye and Batth, {Tanveer S.} and Patrick R{\"u}ther and Olsen, {Jesper V.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
doi = "10.1038/s42003-022-03095-4",
language = "English",
volume = "5",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - A deeper look at carrier proteome effects for single-cell proteomics

AU - Ye, Zilu

AU - Batth, Tanveer S.

AU - Rüther, Patrick

AU - Olsen, Jesper V.

N1 - Publisher Copyright: © 2022, The Author(s).

PY - 2022

Y1 - 2022

N2 - Multiplexing approaches using tandem mass tags with a carrier proteome to boost sensitivity have advanced single cell proteomics by mass spectrometry (SCoPE-MS). Here, we probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins. Guidelines for optimized mass spectrometry acquisition parameters and best practices for fold-change or protein copy number-based comparisons are provided.

AB - Multiplexing approaches using tandem mass tags with a carrier proteome to boost sensitivity have advanced single cell proteomics by mass spectrometry (SCoPE-MS). Here, we probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins. Guidelines for optimized mass spectrometry acquisition parameters and best practices for fold-change or protein copy number-based comparisons are provided.

U2 - 10.1038/s42003-022-03095-4

DO - 10.1038/s42003-022-03095-4

M3 - Journal article

C2 - 35194133

AN - SCOPUS:85125155023

VL - 5

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

M1 - 150

ER -

ID: 300772814