Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Standard

Class 2 CRISPR-Cas RNA-guided endonucleases : Swiss Army knives of genome editing. / Stella, Stefano; Alcón, Pablo; Montoya, Guillermo.

I: Nature Structural and Molecular Biology, Bind 24, Nr. 11, 11.2017, s. 882-892.

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Harvard

Stella, S, Alcón, P & Montoya, G 2017, 'Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing', Nature Structural and Molecular Biology, bind 24, nr. 11, s. 882-892. https://doi.org/10.1038/nsmb.3486

APA

Stella, S., Alcón, P., & Montoya, G. (2017). Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing. Nature Structural and Molecular Biology, 24(11), 882-892. https://doi.org/10.1038/nsmb.3486

Vancouver

Stella S, Alcón P, Montoya G. Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing. Nature Structural and Molecular Biology. 2017 nov.;24(11):882-892. https://doi.org/10.1038/nsmb.3486

Author

Stella, Stefano ; Alcón, Pablo ; Montoya, Guillermo. / Class 2 CRISPR-Cas RNA-guided endonucleases : Swiss Army knives of genome editing. I: Nature Structural and Molecular Biology. 2017 ; Bind 24, Nr. 11. s. 882-892.

Bibtex

@article{6d94e8405278469eb09af67262aa4e1a,
title = "Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing",
abstract = "CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.",
keywords = "CRISPR-Cas Systems, Endonucleases, Gene Editing, RNA, Recombinant Proteins",
author = "Stefano Stella and Pablo Alc{\'o}n and Guillermo Montoya",
year = "2017",
month = nov,
doi = "10.1038/nsmb.3486",
language = "English",
volume = "24",
pages = "882--892",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "nature publishing group",
number = "11",

}

RIS

TY - JOUR

T1 - Class 2 CRISPR-Cas RNA-guided endonucleases

T2 - Swiss Army knives of genome editing

AU - Stella, Stefano

AU - Alcón, Pablo

AU - Montoya, Guillermo

PY - 2017/11

Y1 - 2017/11

N2 - CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.

AB - CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.

KW - CRISPR-Cas Systems

KW - Endonucleases

KW - Gene Editing

KW - RNA

KW - Recombinant Proteins

U2 - 10.1038/nsmb.3486

DO - 10.1038/nsmb.3486

M3 - Review

C2 - 29035385

VL - 24

SP - 882

EP - 892

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 11

ER -

ID: 186152984