TY - JOUR

T1 - A human gut microbial gene catalogue established by metagenomic sequencing

AU - Qin, Junjie

AU - Li, Ruiqiang

AU - Raes, Jeroen

AU - Arumugam, Manimozhiyan

AU - Burgdorf, Kristoffer Solvsten

AU - Manichanh, Chaysavanh

AU - Nielsen, Trine

AU - Pons, Nicolas

AU - Levenez, Florence

AU - Yamada, Takuji

AU - Mende, Daniel R.

AU - Li, Junhua

AU - Xu, Junming

AU - Li, Shaochuan

AU - Li, Dongfang

AU - Cao, Jianjun

AU - Wang, Bo

AU - Liang, Huiqing

AU - Zheng, Huisong

AU - Xie, Yinlong

AU - Tap, Julien

AU - Lepage, Patricia

AU - dos Santos, Marcelo Bertalan Quintanilha

AU - Batto, Jean-Michel

AU - Hansen, Torben

AU - Le Paslier, Denis

AU - Linneberg, Allan René

AU - Nielsen, H. Bjørn

AU - Pelletier, Eric

AU - Renault, Pierre

AU - Sicheritz-Ponten, Thomas

AU - Turner, Keith

AU - Zhu, Hongmei

AU - Yu, Chang

AU - Li, Shengting

AU - Jian, Min

AU - Zhou, Yan

AU - Li, Yingrui

AU - Zhang, Xiuqing

AU - Li, Songgang

AU - Qin, Nan

AU - Yang, Huanming

AU - Wang, Jian

AU - Brunak, Søren

AU - Doré, Joel

AU - Guarner, Francisco

AU - Kristiansen, Karsten

AU - Pedersen, Oluf Borbye

AU - Parkhill, Julian

AU - MetaHIT Consortium

AU - Wang, Jun

N1 - Keywords: Adult; Bacteria; Cohort Studies; Contig Mapping; Denmark; Feces; Gastrointestinal Tract; Genes, Bacterial; Genes, Essential; Genome, Bacterial; Genomics; Health; Humans; Inflammatory Bowel Diseases; Metagenome; Obesity; Open Reading Frames; Overweight; Sequence Analysis, DNA; Spain

PY - 2010

Y1 - 2010

N2 - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

AB - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

U2 - 10.1038/nature08821

DO - 10.1038/nature08821

M3 - Journal article

C2 - 20203603

VL - 464

SP - 59

EP - 65

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7285

ER -