Guillermo Montoya

Guillermo Montoya

Research director, professor


  1. 2020
  2. Published

    Structural basis of CRISPR-Cas Type III prokaryotic defence systems

    Molina, Rafael, Sofos, N. & Montoya, Guillermo, Dec 2020, In : Current Opinion in Structural Biology. 65, p. 119-129 11 p.

    Research output: Contribution to journalReviewResearchpeer-review

  3. E-pub ahead of print

    Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas

    Sofos, N., Feng, M., Stella, Stefano, Pape, T., Fuglsang, Anders, Lin, Jinzhong, Huang, Q., Li, Y., She, Q. & Montoya, Guillermo, 24 Jul 2020, In : Molecular Cell.

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Published

    Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity

    Stella, Stefano, Mesa, Pablo, Thomsen, J., Paul, Bijoya, Alcon, P., Jensen, Simon Bo, Moses, M. E., Montoya, Guillermo & Hatzakis, Nikos, 7 Feb 2020, In : Biophysical Journal. 118, 3, p. 223A-224A 2 p.

    Research output: Contribution to journalConference abstract in journalResearch

  5. Published

    CRISPR-Cas12a: functional overview and applications

    Paul, Bijoya & Montoya, Guillermo, 2020, In : Biomedical Journal. 43, 1, p. 8-17

    Research output: Contribution to journalReviewResearchpeer-review

  6. Published

    Molecular Basis of the Mechanisms Controlling MASTL

    Hermida, D., Mortuza, G. B., Pedersen, A., Pozdnyakova, I., Nguyen, T. T. T. N., Maroto, M., Williamson, M., Ebersole, T., Cazzamali, G., Rand, Kasper Dyrberg, Olsen, Jesper Velgaard, Malumbres, M. & Montoya, Guillermo, 2020, In : Molecular & Cellular Proteomics. 19, 2, p. 326-343

    Research output: Contribution to journalJournal articleResearchpeer-review

  7. 2019
  8. Published

    A Consensus Binding Motif for the PP4 Protein Phosphatase

    Ueki, Yumi, Kruse, Thomas, Weisser, Melanie, Sundell, G. N., Larsen, M. S. Y., Mendez, B. L., Jenkins, N. P., Garvanska, Dimitriya Hristoforova, Cressey, L., Zhang, G., Davey, N., Montoya, Guillermo, Ivarsson, Y., Kettenbach, A. N. & Nilsson, Jakob, 2019, In : Molecular Cell. 76, 6, p. 953-964.e6

    Research output: Contribution to journalJournal articleResearchpeer-review

  9. Published

    High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage

    Slaymaker, I. M., Mesa, Pablo, Kellner, M. J., Kannan, S., Brignole, E., Koob, J., Feliciano, P. R., Stella, Stefano, Abudayyeh, O. O., Gootenberg, J. S., Strecker, J., Montoya, Guillermo & Zhang, F., 2019, In : Cell Reports. 26, 13, p. 3741-3751.e5 17 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  10. Published

    Molecular architecture of the Jumonji C family histone demethylase KDM5B

    Dorosz, J., Kristensen, L. H., Aduri, N. G., Mirza, Osman Asghar, Lousen, R., Bucciarelli, S., Mehta, V., Sellés Baiget, Selene, Solbak, Sara Marie Øie, Bach, A., Mesa, Pablo, Hernandez, P. A., Montoya, Guillermo, Nguyen, T. T. T. N., Rand, Kasper Dyrberg, Boesen, T. & Gajhede, Michael, 2019, In : Scientific Reports. 9, 13 p., 4019.

    Research output: Contribution to journalJournal articleResearchpeer-review

  11. Published

    Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites

    Lundby, A., Franciosa, Giulia, Emdal, Kristina Bennet, Refsgaard, J. C., Gnosa, Sebastian Peter, Bekker-Jensen, D. B., Secher, A., Maurya, S. R., Paul, I., Mendez, B. L., Kelstrup, C. D., Francavilla, C., Kveiborg, Marie, Montoya, Guillermo, Jensen, Lars Juhl & Olsen, Jesper Velgaard, 2019, In : Cell. 179, 2, p. 543-560

    Research output: Contribution to journalJournal articleResearchpeer-review

  12. Published

    Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas

    Molina, Rafael, Stella, Stefano, Feng, M., Sofos, N., Jauniskis, V., Pozdnyakova, I., López-Méndez, B., She, Q. & Montoya, Guillermo, 2019, In : Nature Communications. 10, 14 p., 4302.

    Research output: Contribution to journalJournal articleResearchpeer-review

  13. 2018
  14. Published

    Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

    Stella, Stefano, Mesa, Pablo, Thomsen, J., Paul, Bijoya, Alcón, P., Jensen, Simon Bo, Saligram, B., Moses, M. E., Hatzakis, Nikos & Montoya, Guillermo, 13 Dec 2018, In : Cell. 175, 7, p. 1856-1871.e21 16 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  15. Published

    A Type III-B Cmr effector complex catalyzes the synthesis of cyclic oligoadenylate second messengers by cooperative substrate binding

    Han, W., Stella, Stefano, Zhang, Y., Guo, Tong, Sulek, Karolina, Peng-Lundgren, L., Montoya, Guillermo & She, Q., 2018, In : Nucleic Acids Research. 46, 19, p. 10319-10330 12 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  16. Published

    How do CRISPR-Cas RNA-guided endonucleases cut specific DNA regions for Genome Editing?

    Montoya, Guillermo, 2018, In : Acta Crystallographica Section A: Foundations and Advances. 74, p. E3 1 p.

    Research output: Contribution to journalConference abstract in journalResearch

  17. Published

    Molecular basis of Tousled-Like Kinase 2 activation

    Mortuza, G. B., Hermida, D., Pedersen, A., Segura-Bayona, S., López-Méndez, B., Redondo, P., Rüther, Patrick, Pozdnyakova, I., Garrote, A. M., Muñoz, I. G., Villamor-Payà, M., Jauset, C., Olsen, Jesper Velgaard, Stracker, T. H. & Montoya, Guillermo, 2018, In : Nature Communications. 9, 1, p. 2535 2535.

    Research output: Contribution to journalJournal articleResearchpeer-review

  18. Published

    Understanding the indirect DNA read-out specificity of I-CreI Meganuclease

    Prieto, J., Redondo, P., López-Méndez, B., D'Abramo, M., Merino, N., Blanco, F. J., Duchateau, P., Montoya, Guillermo & Molina, Rafael, 2018, In : Scientific Reports. 8, p. 1-9 10286.

    Research output: Contribution to journalJournal articleResearchpeer-review

  19. 2017
  20. Published

    Structure and dynamics of mesophilic variants from the homing endonuclease I-DmoI

    Alba, J., Marcaida, M. J., Prieto, J., Montoya, Guillermo, Molina, Rafael & D'Abramo, M., Dec 2017, In : Journal of Computer - Aided Molecular Design. 31, 12, p. 1063-1072 10 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  21. Published

    Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing

    Stella, Stefano, Alcón, P. & Montoya, Guillermo, Nov 2017, In : Nature Structural and Molecular Biology. 24, 11, p. 882-892 11 p.

    Research output: Contribution to journalReviewResearchpeer-review

  22. Published

    Allosteric regulation of Csx1, a type IIIB-associated CARF domain ribonuclease by RNAs carrying a tetraadenylate tail

    Han, W., Pan, S., López-Méndez, B., Montoya, Guillermo & She, Q., 13 Oct 2017, In : Nucleic Acids Research. 45, 18, p. 10740-10750 11 p., gkx726.

    Research output: Contribution to journalJournal articleResearchpeer-review

  23. Published

    Assembly of Francisella novicida Cpf1 endonuclease in complex with guide RNA and target DNA

    Alcón, P., Montoya, Guillermo & Stella, Stefano, 1 Jul 2017, In : Acta Crystallographica Section F: Structural Biology Communications. 73, Pt 7, p. 409-415 7 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  24. Published

    Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage

    Stella, Stefano, Alcón, P. & Montoya, Guillermo, 22 Jun 2017, In : Nature. 546, 7659, p. 559-563 5 p.

    Research output: Contribution to journalLetterResearchpeer-review

  25. Published

    A novel TPR-BEN domain interaction mediates PICH-BEND3 association

    Pitchai, G. P., Kaulich, M., Bizard, Anna Helene, Mesa, Pablo, Yao, Q., Sarlos, K., Streicher, W. W., Nigg, E. A., Montoya, Guillermo & Hickson, Ian David, 2017, In : Nucleic Acids Research. 19, 2, p. 11413–11424

    Research output: Contribution to journalJournal articleResearchpeer-review

  26. Published

    Electron Microscopy Structural Insights into CPAP Oligomeric Behavior: A Plausible Assembly Process of a Supramolecular Scaffold of the Centrosome

    Alvarez-Cabrera, A. L., Delgado, S., Gil-Carton, D., Mortuza, G. B., Montoya, Guillermo, Sorzano, C. O. S., Tang, T. K. & Carazo, J. M., 2017, In : Frontiers in Molecular Biosciences. 4, 14 p., 17.

    Research output: Contribution to journalJournal articleResearchpeer-review

  27. 2016
  28. Published

    Molecular architecture of the recombinant human MCM2-7 helicase in complex with nucleotides and DNA

    Boskovic, J., Bragado-Nilsson, E., Saligram Prabhakar, B., Yefimenko, I., Martínez-Gago, J., Muñoz, S., Méndez, J. & Montoya, Guillermo, 16 Sep 2016, In : Cell Cycle. 15, 18, p. 2431-40 10 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  29. Published

    Characterization of the NTPR and BD1 interacting domains of the human PICH-BEND3 complex

    Pitchai, G. P., Hickson, Ian David, Streicher, W., Montoya, Guillermo & Mesa, Pablo, Aug 2016, In : Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online. 72, 8, p. 646-651 6 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  30. Published

    The genome editing revolution: A CRISPR-Cas TALE off-target story

    Stella, Stefano & Montoya, Guillermo, Jul 2016, In : BioEssays. 38, S1, p. S4-S13

    Research output: Contribution to journalJournal articleResearchpeer-review

  31. Published

    Structure of the I-SceI nuclease complexed with its dsDNA target and three catalytic metal ions

    Prieto, J., Redondo, P., Merino, N., Villate, M., Montoya, Guillermo, Blanco, F. J. & Molina, Rafael, Jun 2016, In : Acta crystallographica. Section F, Structural biology communications. 72, Pt 6, p. 473-9 7 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  32. Published

    Key Players in I-DmoI Endonuclease Catalysis Revealed from Structure and Dynamics

    Molina, Rafael, Besker, N., Marcaida, M. J., Montoya, Guillermo, Prieto, J. & D'Abramo, M., 8 Mar 2016, In : ACS chemical biology. 11, 5, p. 1401–1407

    Research output: Contribution to journalJournal articleResearchpeer-review

  33. Published

    A PTIP-PA1 subcomplex promotes transcription for IgH class switching independently from the associated MLL3/MLL4 methyltransferase complex

    Starnes, L. M., Su, D., Pikkupeura, L. M., Weinert, B. T., Santos, M. A., Mund, Andreas, Soria, R., Cho, Y., Pozdnyakova, I., Kubec Højfeldt, M., Lages Lino Vala, A., Yang, W., López-Méndez, B., Lee, J., Peng, W., Yuan, J., Ge, K., Montoya, Guillermo, Nussenzweig, A., Choudhary, Chuna Ram & Daniel, J. A., 15 Jan 2016, In : Genes & Development. 30, 2, p. 149-163 15 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  34. Published

    TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress

    Hoffmann, Saskia, Smedegaard, S., Nakamura, Kyosuke, Mortuza, G. B., Räschle, M., Ibañez de Opakua, A., Oka, Y., Feng, Y., Blanco, F. J., Mann, Matthias, Montoya, Guillermo, Groth, Anja, Bekker-Jensen, Simon Holst & Mailand, Niels, 4 Jan 2016, In : The Journal of Cell Biology. 212, 1, p. 63-75

    Research output: Contribution to journalJournal articleResearchpeer-review

  35. Published

    A Conserved Motif Provides Binding Specificity to the PP2A-B56 Phosphatase

    Hertz, Emil Peter Thrane, Kruse, Thomas, Davey, N. E., López-Méndez, B., Sigurdsson, J. O., Montoya, Guillermo, Olsen, Jesper Velgaard & Nilsson, Jakob, 2016, In : Molecular Cell. 63, 4, p. 686-695 10 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  36. 2015
  37. Published

    Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification

    Molina, Rafael, Redondo, P., López-Méndez, B., Villate, M., Merino, N., Blanco, F. J., Valton, J., Grizot, S., Duchateau, P., Prieto, J. & Montoya, Guillermo, 27 Nov 2015, In : The Journal of Biological Chemistry. 290, 48, p. 28727-36 10 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  38. Published

    Functional and in silico assessment of MAX variants of unknown significance

    Comino-Méndez, I., Leandro-García, L. J., Montoya, Guillermo, Inglada-Pérez, L., de Cubas, A. A., Currás-Freixes, M., Tysoe, C., Izatt, L., Letón, R., Gómez-Graña, Á., Mancikova, V., Apellániz-Ruiz, M., Mannelli, M., Schiavi, F., Favier, J., Gimenez-Roqueplo, A., Timmers, H. J. L. M., Roncador, G., Garcia, J. F., Rodríguez-Antona, C., Robledo, M. & Cascón, A., Nov 2015, In : Journal of Molecular Medicine. 93, 11, p. 1247-55 9 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  39. Published

    Structure of p15PAF-PCNA complex and implications for clamp sliding during DNA replication and repair

    De Biasio, A., de Opakua, A. I., Mortuza, G. B., Molina, Rafael, Cordeiro, T. N., Castillo, F., Villate, M., Merino, N., Delgado, S., Gil-Cartón, D., Luque, I., Diercks, T., Bernadó, P., Montoya, Guillermo & Blanco, F. J., 12 Mar 2015, In : Nature Communications. 6, p. 1-12 6439.

    Research output: Contribution to journalJournal articleResearchpeer-review

  40. Published

    Visualizing phosphodiester-bond hydrolysis by an endonuclease

    Molina, Rafael, Stella, Stefano, Redondo, P., Gomez, H., Marcaida, M. J., Orozco, M., Prieto, J. & Montoya, Guillermo, Jan 2015, In : Nature Structural and Molecular Biology. 22, 1, p. 65-72 8 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  41. Published

    Engineering a Nickase on the Homing Endonuclease I-DmoI Scaffold

    Molina, Rafael, Marcaida, M. J., Redondo, P., Marenchino, M., Duchateau, P., D'Abramo, M., Montoya, Guillermo & Prieto, J., 2015, In : The Journal of Biological Chemistry. 290, 30, p. 18534-18544 11 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  42. 2014
  43. Published

    XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation

    Mortuza, G. B., Cavazza, T., Garcia-Mayoral, M. F., Hermida, D., Peset, I., Pedrero, J. G., Merino, N., Blanco, F. J., Lyngsø, J., Bruix, M., Pedersen, J. S., Vernos, I. & Montoya, Guillermo, 29 Sep 2014, In : Nature Communications. 5, p. 1-12 12 p., 5072.

    Research output: Contribution to journalJournal articleResearchpeer-review

  44. Published

    Comprehensive analysis of the specificity of transcription activator-like effector nucleases

    Juillerat, A., Dubois, G., Valton, J., Thomas, S., Stella, Stefano, Maréchal, A., Langevin, S., Benomari, N., Bertonati, C., Silva, G. H., Daboussi, F., Epinat, J., Montoya, Guillermo, Duclert, A. & Duchateau, P., Apr 2014, In : Nucleic Acids Research. 42, 8, p. 5390-402 13 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  45. Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from Chlorella vulgaris in complex with its target DNA

    Redondo, P., Merino, N., Villate, M., Blanco, F. J., Montoya, Guillermo & Molina, Rafael, Feb 2014, In : Acta crystallographica. Section F, Structural biology communications. 70, Pt 2, p. 256-9 4 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  46. Published

    BuD, a helix-loop-helix DNA-binding domain for genome modification

    Stella, Stefano, Molina, Rafael, López-Méndez, B., Juillerat, A., Bertonati, C., Daboussi, F., Campos-Olivas, R., Duchateau, P. & Montoya, Guillermo, 1 Jan 2014, In : Acta Crystallographica Section D: Biological Crystallography. 70, 7, p. 2042-2052 11 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  47. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the novel modular DNA-binding protein BurrH in its apo form and in complex with its target DNA

    Stella, Stefano, Molina, Rafael, Bertonatti, C., Juillerrat, A. & Montoya, Guillermo, 1 Jan 2014, In : Acta Crystallographica Section F:Structural Biology Communications. 70, 1, p. 87-91 5 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  48. 2013
  49. Directly from the source: endogenous preparations of molecular machines

    Mesa, Pablo, Deniaud, A., Montoya, Guillermo & Schaffitzel, C., Jun 2013, In : Current Opinion in Structural Biology. 23, 3, p. 319-25 7 p.

    Research output: Contribution to journalReviewResearchpeer-review

  50. Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism

    Stella, Stefano, Molina, Rafael, Yefimenko, I., Prieto, J., Silva, G., Bertonati, C., Juillerat, A., Duchateau, P. & Montoya, Guillermo, 2013, In : Acta Crystallographica Section D: Biological Crystallography. 69, 9, p. 1707-1716 10 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  51. 2012
  52. 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases

    Valton, J., Daboussi, F., Leduc, S., Molina, Rafael, Redondo, P., Macmaster, R., Montoya, Guillermo & Duchateau, P., 31 Aug 2012, In : The Journal of Biological Chemistry. 287, 36, p. 30139-50 12 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  53. Published

    Non-specific protein-DNA interactions control I-CreI target binding and cleavage

    Molina, Rafael, Redondo, P., Stella, Stefano, Marenchino, M., D'Abramo, M., Gervasio, F. L., Charles Epinat, J., Valton, J., Grizot, S., Duchateau, P., Prieto, J. & Montoya, Guillermo, 1 Aug 2012, In : Nucleic Acids Research. 40, 14, p. 6936-6945 10 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  54. Molecular scissors for in situ cellular repair

    Prieto, J., Molina, Rafael & Montoya, Guillermo, 31 Jan 2012, In : Critical Reviews in Biochemistry and Molecular Biology. 47, 3, p. 207-21 15 p.

    Research output: Contribution to journalReviewResearchpeer-review

  55. 2011
  56. Published

    Crystal structure of the open conformation of the mammalian chaperonin CCT in complex with tubulin

    Muñoz, I. G., Yébenes, H., Zhou, M., Mesa, Pablo, Serna, M., Park, A. Y., Bragado-Nilsson, E., Beloso, A., de Cárcer, G., Malumbres, M., Robinson, C. V., Valpuesta, J. M. & Montoya, Guillermo, Jan 2011, In : Nature Structural & Molecular Biology. 18, 1, p. 14-9 6 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

  57. 2008
  58. Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases

    Redondo, P., Prieto, J., Muñoz, I. G., Alibés, A., Stricher, F., Serrano, L., Cabaniols, J., Daboussi, F., Arnould, S., Perez, C., Duchateau, P., Pâques, F., Blanco, F. J. & Montoya, Guillermo, 6 Nov 2008, In : Nature. 456, 7218, p. 107-11 5 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

ID: 93716435