Plasma Proteome Profiling to Assess Human Health and Disease
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Plasma Proteome Profiling to Assess Human Health and Disease. / Geyer, Philipp E; Kulak, Nils A; Pichler, Garwin; Holdt, Lesca M; Teupser, Daniel; Mann, Matthias.
In: Cell Systems, Vol. 2, No. 3, 23.03.2016, p. 185-95.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plasma Proteome Profiling to Assess Human Health and Disease
AU - Geyer, Philipp E
AU - Kulak, Nils A
AU - Pichler, Garwin
AU - Holdt, Lesca M
AU - Teupser, Daniel
AU - Mann, Matthias
N1 - Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2016/3/23
Y1 - 2016/3/23
N2 - Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis using mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because of the high dynamic range of protein abundances. Here, we introduce a rapid and robust "plasma proteome profiling" pipeline. This single-run shotgun proteomic workflow does not require protein depletion and enables quantitative analysis of hundreds of plasma proteomes from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV <20% with label-free quantification). Furthermore, we functionally interpret a 1,000-protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. Plasma proteome profiling delivers an informative portrait of a person's health state, and we envision its large-scale use in biomedicine.
AB - Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis using mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because of the high dynamic range of protein abundances. Here, we introduce a rapid and robust "plasma proteome profiling" pipeline. This single-run shotgun proteomic workflow does not require protein depletion and enables quantitative analysis of hundreds of plasma proteomes from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV <20% with label-free quantification). Furthermore, we functionally interpret a 1,000-protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. Plasma proteome profiling delivers an informative portrait of a person's health state, and we envision its large-scale use in biomedicine.
KW - Journal Article
U2 - 10.1016/j.cels.2016.02.015
DO - 10.1016/j.cels.2016.02.015
M3 - Journal article
C2 - 27135364
VL - 2
SP - 185
EP - 195
JO - Cell Systems
JF - Cell Systems
SN - 2405-4712
IS - 3
ER -
ID: 165939283