Analysis and Interpretation of Protein Post-Translational Modification Site Stoichiometry
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Analysis and Interpretation of Protein Post-Translational Modification Site Stoichiometry. / Prus, Gabriela; Hoegl, Annabelle; Weinert, Brian T; Choudhary, Chunaram.
In: Trends in Biochemical Sciences, Vol. 44, No. 11, 2019, p. 943-960.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Analysis and Interpretation of Protein Post-Translational Modification Site Stoichiometry
AU - Prus, Gabriela
AU - Hoegl, Annabelle
AU - Weinert, Brian T
AU - Choudhary, Chunaram
PY - 2019
Y1 - 2019
N2 - Proteins are decorated with a diverse array of post-translational modifications (PTMs) that regulate their spatial and temporal functions. Recent mass spectrometry (MS)-based studies have identified hundreds of thousands of PTM sites in mammalian proteomes. However, the signaling cues and enzymes regulating individual sites are often not known and their functional roles remain uncharacterized. Quantification of PTM site stoichiometry can help in prioritizing sites for functional analyses and is important for constructing mechanistic models of PTM-dependent protein regulation. Here, we review the concept of PTM site stoichiometry, critically evaluate the merits and drawbacks of different MS-based methods used for quantifying PTM site stoichiometry, and discuss the usefulness and limitations of stoichiometry in informing on the biological function of modified sites.
AB - Proteins are decorated with a diverse array of post-translational modifications (PTMs) that regulate their spatial and temporal functions. Recent mass spectrometry (MS)-based studies have identified hundreds of thousands of PTM sites in mammalian proteomes. However, the signaling cues and enzymes regulating individual sites are often not known and their functional roles remain uncharacterized. Quantification of PTM site stoichiometry can help in prioritizing sites for functional analyses and is important for constructing mechanistic models of PTM-dependent protein regulation. Here, we review the concept of PTM site stoichiometry, critically evaluate the merits and drawbacks of different MS-based methods used for quantifying PTM site stoichiometry, and discuss the usefulness and limitations of stoichiometry in informing on the biological function of modified sites.
U2 - 10.1016/j.tibs.2019.06.003
DO - 10.1016/j.tibs.2019.06.003
M3 - Review
C2 - 31296352
VL - 44
SP - 943
EP - 960
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
SN - 0968-0004
IS - 11
ER -
ID: 227087947