Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids. / Olsen, Jesper Velgaard; Haselmann, K F; Nielsen, Michael Lund; Budnik, B A; Nielsen, Peter E.; Zubarev, R A.

In: Rapid Communications in Mass Spectrometry, Vol. 15, No. 12, 2001, p. 969-74.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olsen, JV, Haselmann, KF, Nielsen, ML, Budnik, BA, Nielsen, PE & Zubarev, RA 2001, 'Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids', Rapid Communications in Mass Spectrometry, vol. 15, no. 12, pp. 969-74. https://doi.org/10.1002/rcm.317

APA

Olsen, J. V., Haselmann, K. F., Nielsen, M. L., Budnik, B. A., Nielsen, P. E., & Zubarev, R. A. (2001). Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids. Rapid Communications in Mass Spectrometry, 15(12), 969-74. https://doi.org/10.1002/rcm.317

Vancouver

Olsen JV, Haselmann KF, Nielsen ML, Budnik BA, Nielsen PE, Zubarev RA. Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids. Rapid Communications in Mass Spectrometry. 2001;15(12):969-74. https://doi.org/10.1002/rcm.317

Author

Olsen, Jesper Velgaard ; Haselmann, K F ; Nielsen, Michael Lund ; Budnik, B A ; Nielsen, Peter E. ; Zubarev, R A. / Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids. In: Rapid Communications in Mass Spectrometry. 2001 ; Vol. 15, No. 12. pp. 969-74.

Bibtex

@article{4184a730eb0511deba73000ea68e967b,
title = "Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids",
abstract = "Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.",
author = "Olsen, {Jesper Velgaard} and Haselmann, {K F} and Nielsen, {Michael Lund} and Budnik, {B A} and Nielsen, {Peter E.} and Zubarev, {R A}",
note = "Keywords: Cations; Cyclotrons; Fourier Analysis; Nucleic Acids; Peptides; Spectrometry, Mass, Electrospray Ionization",
year = "2001",
doi = "10.1002/rcm.317",
language = "English",
volume = "15",
pages = "969--74",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "JohnWiley & Sons Ltd",
number = "12",

}

RIS

TY - JOUR

T1 - Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids

AU - Olsen, Jesper Velgaard

AU - Haselmann, K F

AU - Nielsen, Michael Lund

AU - Budnik, B A

AU - Nielsen, Peter E.

AU - Zubarev, R A

N1 - Keywords: Cations; Cyclotrons; Fourier Analysis; Nucleic Acids; Peptides; Spectrometry, Mass, Electrospray Ionization

PY - 2001

Y1 - 2001

N2 - Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.

AB - Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.

U2 - 10.1002/rcm.317

DO - 10.1002/rcm.317

M3 - Journal article

C2 - 11400205

VL - 15

SP - 969

EP - 974

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 12

ER -

ID: 16330738