Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. / Gallina, Irene; Colding, Camilla Skettrup; Henriksen, Peter; Beli, Petra; Nakamura, Kyosuke; Offman, Judith; Barfred, David Plesner; Pinela da Silva, Sonia Cristina; Hoffmann, Eva; Groth, Anja; Choudhary, Chunaram; Lisby, Michael.

In: Nature Communications, Vol. 6, 6533, 2015.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gallina, I, Colding, CS, Henriksen, P, Beli, P, Nakamura, K, Offman, J, Barfred, DP, Pinela da Silva, SC, Hoffmann, E, Groth, A, Choudhary, C & Lisby, M 2015, 'Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control', Nature Communications, vol. 6, 6533. https://doi.org/10.1038/ncomms7533

APA

Gallina, I., Colding, C. S., Henriksen, P., Beli, P., Nakamura, K., Offman, J., Barfred, D. P., Pinela da Silva, S. C., Hoffmann, E., Groth, A., Choudhary, C., & Lisby, M. (2015). Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. Nature Communications, 6, [6533]. https://doi.org/10.1038/ncomms7533

Vancouver

Gallina I, Colding CS, Henriksen P, Beli P, Nakamura K, Offman J et al. Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. Nature Communications. 2015;6. 6533. https://doi.org/10.1038/ncomms7533

Author

Gallina, Irene ; Colding, Camilla Skettrup ; Henriksen, Peter ; Beli, Petra ; Nakamura, Kyosuke ; Offman, Judith ; Barfred, David Plesner ; Pinela da Silva, Sonia Cristina ; Hoffmann, Eva ; Groth, Anja ; Choudhary, Chunaram ; Lisby, Michael. / Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. In: Nature Communications. 2015 ; Vol. 6.

Bibtex

@article{8cc86ac86dcd4565a97cdb6627c00751,
title = "Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control",
abstract = "DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1-together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins-define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.",
author = "Irene Gallina and Colding, {Camilla Skettrup} and Peter Henriksen and Petra Beli and Kyosuke Nakamura and Judith Offman and Barfred, {David Plesner} and {Pinela da Silva}, {Sonia Cristina} and Eva Hoffmann and Anja Groth and Chunaram Choudhary and Michael Lisby",
year = "2015",
doi = "10.1038/ncomms7533",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control

AU - Gallina, Irene

AU - Colding, Camilla Skettrup

AU - Henriksen, Peter

AU - Beli, Petra

AU - Nakamura, Kyosuke

AU - Offman, Judith

AU - Barfred, David Plesner

AU - Pinela da Silva, Sonia Cristina

AU - Hoffmann, Eva

AU - Groth, Anja

AU - Choudhary, Chunaram

AU - Lisby, Michael

PY - 2015

Y1 - 2015

N2 - DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1-together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins-define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.

AB - DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1-together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins-define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.

U2 - 10.1038/ncomms7533

DO - 10.1038/ncomms7533

M3 - Journal article

C2 - 25817432

VL - 6

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 6533

ER -

ID: 134992094