Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p

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Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p. / Madsen, Christian Toft; Sylvestersen, Kathrine Beck; Young, Clifford; Larsen, Sara Charlotte; Poulsen, Jon Wriedt; Andersen, Marianne Agerholm; Palmqvist, Eva A; Hey-Mogensen, Martin; Jensen, Per B.; Treebak, Jonas Thue; Lisby, Michael; Nielsen, Michael Lund.

In: Nature Communications, Vol. 6, 7726, 2015.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Madsen, CT, Sylvestersen, KB, Young, C, Larsen, SC, Poulsen, JW, Andersen, MA, Palmqvist, EA, Hey-Mogensen, M, Jensen, PB, Treebak, JT, Lisby, M & Nielsen, ML 2015, 'Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p', Nature Communications, vol. 6, 7726. https://doi.org/10.1038/ncomms8726

APA

Madsen, C. T., Sylvestersen, K. B., Young, C., Larsen, S. C., Poulsen, J. W., Andersen, M. A., ... Nielsen, M. L. (2015). Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p. Nature Communications, 6, [7726]. https://doi.org/10.1038/ncomms8726

Vancouver

Madsen CT, Sylvestersen KB, Young C, Larsen SC, Poulsen JW, Andersen MA et al. Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p. Nature Communications. 2015;6. 7726. https://doi.org/10.1038/ncomms8726

Author

Madsen, Christian Toft ; Sylvestersen, Kathrine Beck ; Young, Clifford ; Larsen, Sara Charlotte ; Poulsen, Jon Wriedt ; Andersen, Marianne Agerholm ; Palmqvist, Eva A ; Hey-Mogensen, Martin ; Jensen, Per B. ; Treebak, Jonas Thue ; Lisby, Michael ; Nielsen, Michael Lund. / Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p. In: Nature Communications. 2015 ; Vol. 6.

Bibtex

@article{fc335c2dc319428d8e891cb0a07f3a3e,
title = "Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p",
abstract = "The essential vitamin biotin is a covalent and tenaciously attached prosthetic group in several carboxylases that play important roles in the regulation of energy metabolism. Here we describe increased acetyl-CoA levels and mitochondrial hyperacetylation as downstream metabolic effects of biotin deficiency. Upregulated mitochondrial acetylation sites correlate with the cellular deficiency of the Hst4p deacetylase, and a biotin-starvation-induced accumulation of Hst4p in mitochondria supports a role for Hst4p in lowering mitochondrial acetylation. We show that biotin starvation and knockout of Hst4p cause alterations in cellular respiration and an increase in reactive oxygen species (ROS). These results suggest that Hst4p plays a pivotal role in biotin metabolism and cellular energy homeostasis, and supports that Hst4p is a functional yeast homologue of the sirtuin deacetylase SIRT3. With biotin deficiency being involved in various metabolic disorders, this study provides valuable insight into the metabolic effects biotin exerts on eukaryotic cells.",
author = "Madsen, {Christian Toft} and Sylvestersen, {Kathrine Beck} and Clifford Young and Larsen, {Sara Charlotte} and Poulsen, {Jon Wriedt} and Andersen, {Marianne Agerholm} and Palmqvist, {Eva A} and Martin Hey-Mogensen and Jensen, {Per B.} and Treebak, {Jonas Thue} and Michael Lisby and Nielsen, {Michael Lund}",
year = "2015",
doi = "10.1038/ncomms8726",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p

AU - Madsen, Christian Toft

AU - Sylvestersen, Kathrine Beck

AU - Young, Clifford

AU - Larsen, Sara Charlotte

AU - Poulsen, Jon Wriedt

AU - Andersen, Marianne Agerholm

AU - Palmqvist, Eva A

AU - Hey-Mogensen, Martin

AU - Jensen, Per B.

AU - Treebak, Jonas Thue

AU - Lisby, Michael

AU - Nielsen, Michael Lund

PY - 2015

Y1 - 2015

N2 - The essential vitamin biotin is a covalent and tenaciously attached prosthetic group in several carboxylases that play important roles in the regulation of energy metabolism. Here we describe increased acetyl-CoA levels and mitochondrial hyperacetylation as downstream metabolic effects of biotin deficiency. Upregulated mitochondrial acetylation sites correlate with the cellular deficiency of the Hst4p deacetylase, and a biotin-starvation-induced accumulation of Hst4p in mitochondria supports a role for Hst4p in lowering mitochondrial acetylation. We show that biotin starvation and knockout of Hst4p cause alterations in cellular respiration and an increase in reactive oxygen species (ROS). These results suggest that Hst4p plays a pivotal role in biotin metabolism and cellular energy homeostasis, and supports that Hst4p is a functional yeast homologue of the sirtuin deacetylase SIRT3. With biotin deficiency being involved in various metabolic disorders, this study provides valuable insight into the metabolic effects biotin exerts on eukaryotic cells.

AB - The essential vitamin biotin is a covalent and tenaciously attached prosthetic group in several carboxylases that play important roles in the regulation of energy metabolism. Here we describe increased acetyl-CoA levels and mitochondrial hyperacetylation as downstream metabolic effects of biotin deficiency. Upregulated mitochondrial acetylation sites correlate with the cellular deficiency of the Hst4p deacetylase, and a biotin-starvation-induced accumulation of Hst4p in mitochondria supports a role for Hst4p in lowering mitochondrial acetylation. We show that biotin starvation and knockout of Hst4p cause alterations in cellular respiration and an increase in reactive oxygen species (ROS). These results suggest that Hst4p plays a pivotal role in biotin metabolism and cellular energy homeostasis, and supports that Hst4p is a functional yeast homologue of the sirtuin deacetylase SIRT3. With biotin deficiency being involved in various metabolic disorders, this study provides valuable insight into the metabolic effects biotin exerts on eukaryotic cells.

U2 - 10.1038/ncomms8726

DO - 10.1038/ncomms8726

M3 - Journal article

C2 - 26158509

VL - 6

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 7726

ER -

ID: 144284901